Cargando…
Assessment of Anti-Human Leukocyte Antigen (HLA)-Antibody-Dependent Humoral Response in Patients before and after Lung Transplantation
Background and Objectives: Testing for anti-human leukocyte antigen (HLA) antibodies both before and after transplantation is of fundamental significance for the success of lung transplantation. The aim of this study was the evaluation of anti-HLA immunization of patients before and after lung trans...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9781897/ https://www.ncbi.nlm.nih.gov/pubmed/36556973 http://dx.doi.org/10.3390/medicina58121771 |
_version_ | 1784857187042983936 |
---|---|
author | Stanjek-Cichoracka, Anita Ochman, Marek Chełmecka, Elżbieta Hrapkowicz, Tomasz |
author_facet | Stanjek-Cichoracka, Anita Ochman, Marek Chełmecka, Elżbieta Hrapkowicz, Tomasz |
author_sort | Stanjek-Cichoracka, Anita |
collection | PubMed |
description | Background and Objectives: Testing for anti-human leukocyte antigen (HLA) antibodies both before and after transplantation is of fundamental significance for the success of lung transplantation. The aim of this study was the evaluation of anti-HLA immunization of patients before and after lung transplant who were subjected to qualification and transplantation. Materials and Methods: Prior to the transplantation, patients were examined for the presence of IgG class anti-HLA antibodies (anti-human leukocyte antigen), the so-called panel-reactive antibodies (PRA), using the flow cytometry method. After the transplantation, the class and specificity of anti-HLA antibodies (also IgG) were determined using Luminex. Results: In the group examined, the PRA results ranged from 0.1% to 66.4%. Low (30%) and average (30–80%) immunization was found in only 9.7% of the group examined. Presence of class I anti-HLA antibodies with MFI (mean fluorescence intensity) greater than 1000 was found in 42.7% of the patients examined, while class II anti-HLA antibodies were found in 38.4%. Immunization levels before and after the transplantation were compared. In 10.87% of patients, DSA antibodies (donor-specific antibodies) with MFI of over 1000 were found. Conclusions: It seems that it is possible to confirm the correlation between pre- and post-transplantation immunization with the use of the two presented methods of determining IgG class anti-HLA antibodies by increasing the size of the group studied and conducting a long-term observation thereof. |
format | Online Article Text |
id | pubmed-9781897 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97818972022-12-24 Assessment of Anti-Human Leukocyte Antigen (HLA)-Antibody-Dependent Humoral Response in Patients before and after Lung Transplantation Stanjek-Cichoracka, Anita Ochman, Marek Chełmecka, Elżbieta Hrapkowicz, Tomasz Medicina (Kaunas) Article Background and Objectives: Testing for anti-human leukocyte antigen (HLA) antibodies both before and after transplantation is of fundamental significance for the success of lung transplantation. The aim of this study was the evaluation of anti-HLA immunization of patients before and after lung transplant who were subjected to qualification and transplantation. Materials and Methods: Prior to the transplantation, patients were examined for the presence of IgG class anti-HLA antibodies (anti-human leukocyte antigen), the so-called panel-reactive antibodies (PRA), using the flow cytometry method. After the transplantation, the class and specificity of anti-HLA antibodies (also IgG) were determined using Luminex. Results: In the group examined, the PRA results ranged from 0.1% to 66.4%. Low (30%) and average (30–80%) immunization was found in only 9.7% of the group examined. Presence of class I anti-HLA antibodies with MFI (mean fluorescence intensity) greater than 1000 was found in 42.7% of the patients examined, while class II anti-HLA antibodies were found in 38.4%. Immunization levels before and after the transplantation were compared. In 10.87% of patients, DSA antibodies (donor-specific antibodies) with MFI of over 1000 were found. Conclusions: It seems that it is possible to confirm the correlation between pre- and post-transplantation immunization with the use of the two presented methods of determining IgG class anti-HLA antibodies by increasing the size of the group studied and conducting a long-term observation thereof. MDPI 2022-11-30 /pmc/articles/PMC9781897/ /pubmed/36556973 http://dx.doi.org/10.3390/medicina58121771 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Stanjek-Cichoracka, Anita Ochman, Marek Chełmecka, Elżbieta Hrapkowicz, Tomasz Assessment of Anti-Human Leukocyte Antigen (HLA)-Antibody-Dependent Humoral Response in Patients before and after Lung Transplantation |
title | Assessment of Anti-Human Leukocyte Antigen (HLA)-Antibody-Dependent Humoral Response in Patients before and after Lung Transplantation |
title_full | Assessment of Anti-Human Leukocyte Antigen (HLA)-Antibody-Dependent Humoral Response in Patients before and after Lung Transplantation |
title_fullStr | Assessment of Anti-Human Leukocyte Antigen (HLA)-Antibody-Dependent Humoral Response in Patients before and after Lung Transplantation |
title_full_unstemmed | Assessment of Anti-Human Leukocyte Antigen (HLA)-Antibody-Dependent Humoral Response in Patients before and after Lung Transplantation |
title_short | Assessment of Anti-Human Leukocyte Antigen (HLA)-Antibody-Dependent Humoral Response in Patients before and after Lung Transplantation |
title_sort | assessment of anti-human leukocyte antigen (hla)-antibody-dependent humoral response in patients before and after lung transplantation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9781897/ https://www.ncbi.nlm.nih.gov/pubmed/36556973 http://dx.doi.org/10.3390/medicina58121771 |
work_keys_str_mv | AT stanjekcichorackaanita assessmentofantihumanleukocyteantigenhlaantibodydependenthumoralresponseinpatientsbeforeandafterlungtransplantation AT ochmanmarek assessmentofantihumanleukocyteantigenhlaantibodydependenthumoralresponseinpatientsbeforeandafterlungtransplantation AT chełmeckaelzbieta assessmentofantihumanleukocyteantigenhlaantibodydependenthumoralresponseinpatientsbeforeandafterlungtransplantation AT hrapkowicztomasz assessmentofantihumanleukocyteantigenhlaantibodydependenthumoralresponseinpatientsbeforeandafterlungtransplantation |