A Sandwich ELISA for Quality Control of PCV2 Virus-like Particles Vaccine

Porcine circovirus type 2 (PCV2) is a highly prevalent virus in pig farms worldwide that causes significant economic losses in the swine industry. The PCV2 virus-like particles (VLPs) are potent subunit vaccines that are widely used. Currently, the adopted quality control of VLPs vaccines is mainly...

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Autores principales: Sun, Mingxia, Wang, Shanghui, Fang, Zheng, Zhao, Man, Gao, Yanfei, An, Tongqing, Tu, Yabin, Wang, Haiwei, Cai, Xuehui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9781900/
https://www.ncbi.nlm.nih.gov/pubmed/36560585
http://dx.doi.org/10.3390/vaccines10122175
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author Sun, Mingxia
Wang, Shanghui
Fang, Zheng
Zhao, Man
Gao, Yanfei
An, Tongqing
Tu, Yabin
Wang, Haiwei
Cai, Xuehui
author_facet Sun, Mingxia
Wang, Shanghui
Fang, Zheng
Zhao, Man
Gao, Yanfei
An, Tongqing
Tu, Yabin
Wang, Haiwei
Cai, Xuehui
author_sort Sun, Mingxia
collection PubMed
description Porcine circovirus type 2 (PCV2) is a highly prevalent virus in pig farms worldwide that causes significant economic losses in the swine industry. The PCV2 virus-like particles (VLPs) are potent subunit vaccines that are widely used. Currently, the adopted quality control of VLPs vaccines is mainly based in animal testing, the titration of neutralizing antibodies, or other biochemical/biophysical assays. In this study, we generated a monoclonal antibody that can distinguish assembled PCV2 VLPs from the capsid proteins. Subsequently, a convenient Sandwich ELISA was developed based on the monoclonal antibody (mAb) that recognizes the PCV2 VLPs specifically. This assay can be used for the quantity and quality control of PCV2 VLPs vaccines for both the intermediate or final products with high accuracy.
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spelling pubmed-97819002022-12-24 A Sandwich ELISA for Quality Control of PCV2 Virus-like Particles Vaccine Sun, Mingxia Wang, Shanghui Fang, Zheng Zhao, Man Gao, Yanfei An, Tongqing Tu, Yabin Wang, Haiwei Cai, Xuehui Vaccines (Basel) Article Porcine circovirus type 2 (PCV2) is a highly prevalent virus in pig farms worldwide that causes significant economic losses in the swine industry. The PCV2 virus-like particles (VLPs) are potent subunit vaccines that are widely used. Currently, the adopted quality control of VLPs vaccines is mainly based in animal testing, the titration of neutralizing antibodies, or other biochemical/biophysical assays. In this study, we generated a monoclonal antibody that can distinguish assembled PCV2 VLPs from the capsid proteins. Subsequently, a convenient Sandwich ELISA was developed based on the monoclonal antibody (mAb) that recognizes the PCV2 VLPs specifically. This assay can be used for the quantity and quality control of PCV2 VLPs vaccines for both the intermediate or final products with high accuracy. MDPI 2022-12-18 /pmc/articles/PMC9781900/ /pubmed/36560585 http://dx.doi.org/10.3390/vaccines10122175 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sun, Mingxia
Wang, Shanghui
Fang, Zheng
Zhao, Man
Gao, Yanfei
An, Tongqing
Tu, Yabin
Wang, Haiwei
Cai, Xuehui
A Sandwich ELISA for Quality Control of PCV2 Virus-like Particles Vaccine
title A Sandwich ELISA for Quality Control of PCV2 Virus-like Particles Vaccine
title_full A Sandwich ELISA for Quality Control of PCV2 Virus-like Particles Vaccine
title_fullStr A Sandwich ELISA for Quality Control of PCV2 Virus-like Particles Vaccine
title_full_unstemmed A Sandwich ELISA for Quality Control of PCV2 Virus-like Particles Vaccine
title_short A Sandwich ELISA for Quality Control of PCV2 Virus-like Particles Vaccine
title_sort sandwich elisa for quality control of pcv2 virus-like particles vaccine
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9781900/
https://www.ncbi.nlm.nih.gov/pubmed/36560585
http://dx.doi.org/10.3390/vaccines10122175
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