Methods for the Assessment of NET Formation: From Neutrophil Biology to Translational Research

Several studies have indicated that a neutrophil extracellular trap (NET) formation, apart from its role in host defense, can contribute to or drive pathogenesis in a wide range of inflammatory and thrombotic disorders. Therefore, NETs may serve as a therapeutic target or/and a diagnostic tool. Here...

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Autores principales: Stoimenou, Marina, Tzoros, Georgios, Skendros, Panagiotis, Chrysanthopoulou, Akrivi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9781911/
https://www.ncbi.nlm.nih.gov/pubmed/36555464
http://dx.doi.org/10.3390/ijms232415823
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author Stoimenou, Marina
Tzoros, Georgios
Skendros, Panagiotis
Chrysanthopoulou, Akrivi
author_facet Stoimenou, Marina
Tzoros, Georgios
Skendros, Panagiotis
Chrysanthopoulou, Akrivi
author_sort Stoimenou, Marina
collection PubMed
description Several studies have indicated that a neutrophil extracellular trap (NET) formation, apart from its role in host defense, can contribute to or drive pathogenesis in a wide range of inflammatory and thrombotic disorders. Therefore, NETs may serve as a therapeutic target or/and a diagnostic tool. Here, we compare the most commonly used techniques for the assessment of NET formation. Furthermore, we review recent data from the literature on the application of basic laboratory tools for detecting NET release and discuss the challenges and the advantages of these strategies in NET evaluation. Taken together, we provide some important insights into the qualitative and quantitative molecular analysis of NETs in translational medicine today.
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spelling pubmed-97819112022-12-24 Methods for the Assessment of NET Formation: From Neutrophil Biology to Translational Research Stoimenou, Marina Tzoros, Georgios Skendros, Panagiotis Chrysanthopoulou, Akrivi Int J Mol Sci Review Several studies have indicated that a neutrophil extracellular trap (NET) formation, apart from its role in host defense, can contribute to or drive pathogenesis in a wide range of inflammatory and thrombotic disorders. Therefore, NETs may serve as a therapeutic target or/and a diagnostic tool. Here, we compare the most commonly used techniques for the assessment of NET formation. Furthermore, we review recent data from the literature on the application of basic laboratory tools for detecting NET release and discuss the challenges and the advantages of these strategies in NET evaluation. Taken together, we provide some important insights into the qualitative and quantitative molecular analysis of NETs in translational medicine today. MDPI 2022-12-13 /pmc/articles/PMC9781911/ /pubmed/36555464 http://dx.doi.org/10.3390/ijms232415823 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Stoimenou, Marina
Tzoros, Georgios
Skendros, Panagiotis
Chrysanthopoulou, Akrivi
Methods for the Assessment of NET Formation: From Neutrophil Biology to Translational Research
title Methods for the Assessment of NET Formation: From Neutrophil Biology to Translational Research
title_full Methods for the Assessment of NET Formation: From Neutrophil Biology to Translational Research
title_fullStr Methods for the Assessment of NET Formation: From Neutrophil Biology to Translational Research
title_full_unstemmed Methods for the Assessment of NET Formation: From Neutrophil Biology to Translational Research
title_short Methods for the Assessment of NET Formation: From Neutrophil Biology to Translational Research
title_sort methods for the assessment of net formation: from neutrophil biology to translational research
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9781911/
https://www.ncbi.nlm.nih.gov/pubmed/36555464
http://dx.doi.org/10.3390/ijms232415823
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