Cargando…

Finding of Novel Galactose Utilizing Halomonas sp. YK44 for Polyhydroxybutyrate (PHB) Production

Polyhydroxybutyrate (PHB) is a biodegradable bioplastic with potential applications as an alternative to petroleum-based plastics. However, efficient PHB production remains difficult. The main cost of PHB production is attributed to carbon sources; hence, finding inexpensive sources is important. Ga...

Descripción completa

Detalles Bibliográficos
Autores principales: Jung, Hee Ju, Kim, Su Hyun, Cho, Do Hyun, Kim, Byung Chan, Bhatia, Shashi Kant, Lee, Jongbok, Jeon, Jong-Min, Yoon, Jeong-Jun, Yang, Yung-Hun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9782037/
https://www.ncbi.nlm.nih.gov/pubmed/36559775
http://dx.doi.org/10.3390/polym14245407
_version_ 1784857236172963840
author Jung, Hee Ju
Kim, Su Hyun
Cho, Do Hyun
Kim, Byung Chan
Bhatia, Shashi Kant
Lee, Jongbok
Jeon, Jong-Min
Yoon, Jeong-Jun
Yang, Yung-Hun
author_facet Jung, Hee Ju
Kim, Su Hyun
Cho, Do Hyun
Kim, Byung Chan
Bhatia, Shashi Kant
Lee, Jongbok
Jeon, Jong-Min
Yoon, Jeong-Jun
Yang, Yung-Hun
author_sort Jung, Hee Ju
collection PubMed
description Polyhydroxybutyrate (PHB) is a biodegradable bioplastic with potential applications as an alternative to petroleum-based plastics. However, efficient PHB production remains difficult. The main cost of PHB production is attributed to carbon sources; hence, finding inexpensive sources is important. Galactose is a possible substrate for polyhydroxyalkanoate production as it is abundant in marine environments. Marine bacteria that produce PHB from galactose could be an effective resource that can be used for efficient PHB production. In this study, to identify a galactose utilizing PHB producer, we examined 16 Halomonas strains. We demonstrated that Halomonas cerina (Halomonas sp. YK44) has the highest growth and PHB production using a culture media containing 2% galactose, final 4% NaCl, and 0.1% yeast extract. These culture conditions yielded 8.98 g/L PHB (78.1% PHB content (w/w)). When galactose-containing red algae (Eucheuma spinosum) hydrolysates were used as a carbon source, 5.2 g/L PHB was produced with 1.425% galactose after treatment with activated carbon. Since high salt conditions can be used to avoid sterilization, we examined whether Halomonas sp. YK44 could produce PHB in non-sterilized conditions. Culture media in these conditions yielded 72.41% PHB content. Thus, Halomonas sp. YK44 is robust against contamination, allowing for long-term culture and economical PHB production.
format Online
Article
Text
id pubmed-9782037
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-97820372022-12-24 Finding of Novel Galactose Utilizing Halomonas sp. YK44 for Polyhydroxybutyrate (PHB) Production Jung, Hee Ju Kim, Su Hyun Cho, Do Hyun Kim, Byung Chan Bhatia, Shashi Kant Lee, Jongbok Jeon, Jong-Min Yoon, Jeong-Jun Yang, Yung-Hun Polymers (Basel) Article Polyhydroxybutyrate (PHB) is a biodegradable bioplastic with potential applications as an alternative to petroleum-based plastics. However, efficient PHB production remains difficult. The main cost of PHB production is attributed to carbon sources; hence, finding inexpensive sources is important. Galactose is a possible substrate for polyhydroxyalkanoate production as it is abundant in marine environments. Marine bacteria that produce PHB from galactose could be an effective resource that can be used for efficient PHB production. In this study, to identify a galactose utilizing PHB producer, we examined 16 Halomonas strains. We demonstrated that Halomonas cerina (Halomonas sp. YK44) has the highest growth and PHB production using a culture media containing 2% galactose, final 4% NaCl, and 0.1% yeast extract. These culture conditions yielded 8.98 g/L PHB (78.1% PHB content (w/w)). When galactose-containing red algae (Eucheuma spinosum) hydrolysates were used as a carbon source, 5.2 g/L PHB was produced with 1.425% galactose after treatment with activated carbon. Since high salt conditions can be used to avoid sterilization, we examined whether Halomonas sp. YK44 could produce PHB in non-sterilized conditions. Culture media in these conditions yielded 72.41% PHB content. Thus, Halomonas sp. YK44 is robust against contamination, allowing for long-term culture and economical PHB production. MDPI 2022-12-10 /pmc/articles/PMC9782037/ /pubmed/36559775 http://dx.doi.org/10.3390/polym14245407 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jung, Hee Ju
Kim, Su Hyun
Cho, Do Hyun
Kim, Byung Chan
Bhatia, Shashi Kant
Lee, Jongbok
Jeon, Jong-Min
Yoon, Jeong-Jun
Yang, Yung-Hun
Finding of Novel Galactose Utilizing Halomonas sp. YK44 for Polyhydroxybutyrate (PHB) Production
title Finding of Novel Galactose Utilizing Halomonas sp. YK44 for Polyhydroxybutyrate (PHB) Production
title_full Finding of Novel Galactose Utilizing Halomonas sp. YK44 for Polyhydroxybutyrate (PHB) Production
title_fullStr Finding of Novel Galactose Utilizing Halomonas sp. YK44 for Polyhydroxybutyrate (PHB) Production
title_full_unstemmed Finding of Novel Galactose Utilizing Halomonas sp. YK44 for Polyhydroxybutyrate (PHB) Production
title_short Finding of Novel Galactose Utilizing Halomonas sp. YK44 for Polyhydroxybutyrate (PHB) Production
title_sort finding of novel galactose utilizing halomonas sp. yk44 for polyhydroxybutyrate (phb) production
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9782037/
https://www.ncbi.nlm.nih.gov/pubmed/36559775
http://dx.doi.org/10.3390/polym14245407
work_keys_str_mv AT jungheeju findingofnovelgalactoseutilizinghalomonasspyk44forpolyhydroxybutyratephbproduction
AT kimsuhyun findingofnovelgalactoseutilizinghalomonasspyk44forpolyhydroxybutyratephbproduction
AT chodohyun findingofnovelgalactoseutilizinghalomonasspyk44forpolyhydroxybutyratephbproduction
AT kimbyungchan findingofnovelgalactoseutilizinghalomonasspyk44forpolyhydroxybutyratephbproduction
AT bhatiashashikant findingofnovelgalactoseutilizinghalomonasspyk44forpolyhydroxybutyratephbproduction
AT leejongbok findingofnovelgalactoseutilizinghalomonasspyk44forpolyhydroxybutyratephbproduction
AT jeonjongmin findingofnovelgalactoseutilizinghalomonasspyk44forpolyhydroxybutyratephbproduction
AT yoonjeongjun findingofnovelgalactoseutilizinghalomonasspyk44forpolyhydroxybutyratephbproduction
AT yangyunghun findingofnovelgalactoseutilizinghalomonasspyk44forpolyhydroxybutyratephbproduction