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Punica granatum Peel and Leaf Extracts as Promising Strategies for HSV-1 Treatment
Punica granatum is a rich source of bioactive compounds which exhibit various biological effects. In this study, pomegranate peel and leaf ethanolic crude extracts (PPE and PLE, respectively) were phytochemically characterized and screened for antioxidant, antimicrobial and antiviral activity. LC-PD...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9782130/ https://www.ncbi.nlm.nih.gov/pubmed/36560643 http://dx.doi.org/10.3390/v14122639 |
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author | EL-Aguel, Asma Pennisi, Rosamaria Smeriglio, Antonella Kallel, Imen Tamburello, Maria Pia D’Arrigo, Manuela Barreca, Davide Gargouri, Ahmed Trombetta, Domenico Mandalari, Giuseppina Sciortino, Maria Teresa |
author_facet | EL-Aguel, Asma Pennisi, Rosamaria Smeriglio, Antonella Kallel, Imen Tamburello, Maria Pia D’Arrigo, Manuela Barreca, Davide Gargouri, Ahmed Trombetta, Domenico Mandalari, Giuseppina Sciortino, Maria Teresa |
author_sort | EL-Aguel, Asma |
collection | PubMed |
description | Punica granatum is a rich source of bioactive compounds which exhibit various biological effects. In this study, pomegranate peel and leaf ethanolic crude extracts (PPE and PLE, respectively) were phytochemically characterized and screened for antioxidant, antimicrobial and antiviral activity. LC-PDA-ESI-MS analysis led to the identification of different compounds, including ellagitannins, flavonoids and phenolic acids. The low IC(50) values, obtained by DPPH and FRAP assays, showed a noticeable antioxidant effect of PPE and PLE comparable to the reference standards. Both crude extracts and their main compounds (gallic acid, ellagic acid and punicalagin) were not toxic on Vero cells and exhibited a remarkable inhibitory effect on herpes simplex type 1 (HSV-1) viral plaques formation. Specifically, PPE inhibited HSV-1 adsorption to the cell surface more than PLE. Indeed, the viral DNA accumulation, the transcription of viral genes and the expression of viral proteins were significantly affected by PPE treatment. Amongst the compounds, punicalagin, which is abundant in PPE crude extract, inhibited HSV-1 replication, reducing viral DNA and transcripts accumulation, as well as proteins of all three phases of the viral replication cascade. In contrast, no antibacterial activity was detected. In conclusion, our findings indicate that Punica granatum peel and leaf extracts, especially punicalagin, could be a promising therapeutic candidate against HSV-1. |
format | Online Article Text |
id | pubmed-9782130 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97821302022-12-24 Punica granatum Peel and Leaf Extracts as Promising Strategies for HSV-1 Treatment EL-Aguel, Asma Pennisi, Rosamaria Smeriglio, Antonella Kallel, Imen Tamburello, Maria Pia D’Arrigo, Manuela Barreca, Davide Gargouri, Ahmed Trombetta, Domenico Mandalari, Giuseppina Sciortino, Maria Teresa Viruses Article Punica granatum is a rich source of bioactive compounds which exhibit various biological effects. In this study, pomegranate peel and leaf ethanolic crude extracts (PPE and PLE, respectively) were phytochemically characterized and screened for antioxidant, antimicrobial and antiviral activity. LC-PDA-ESI-MS analysis led to the identification of different compounds, including ellagitannins, flavonoids and phenolic acids. The low IC(50) values, obtained by DPPH and FRAP assays, showed a noticeable antioxidant effect of PPE and PLE comparable to the reference standards. Both crude extracts and their main compounds (gallic acid, ellagic acid and punicalagin) were not toxic on Vero cells and exhibited a remarkable inhibitory effect on herpes simplex type 1 (HSV-1) viral plaques formation. Specifically, PPE inhibited HSV-1 adsorption to the cell surface more than PLE. Indeed, the viral DNA accumulation, the transcription of viral genes and the expression of viral proteins were significantly affected by PPE treatment. Amongst the compounds, punicalagin, which is abundant in PPE crude extract, inhibited HSV-1 replication, reducing viral DNA and transcripts accumulation, as well as proteins of all three phases of the viral replication cascade. In contrast, no antibacterial activity was detected. In conclusion, our findings indicate that Punica granatum peel and leaf extracts, especially punicalagin, could be a promising therapeutic candidate against HSV-1. MDPI 2022-11-26 /pmc/articles/PMC9782130/ /pubmed/36560643 http://dx.doi.org/10.3390/v14122639 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article EL-Aguel, Asma Pennisi, Rosamaria Smeriglio, Antonella Kallel, Imen Tamburello, Maria Pia D’Arrigo, Manuela Barreca, Davide Gargouri, Ahmed Trombetta, Domenico Mandalari, Giuseppina Sciortino, Maria Teresa Punica granatum Peel and Leaf Extracts as Promising Strategies for HSV-1 Treatment |
title | Punica granatum Peel and Leaf Extracts as Promising Strategies for HSV-1 Treatment |
title_full | Punica granatum Peel and Leaf Extracts as Promising Strategies for HSV-1 Treatment |
title_fullStr | Punica granatum Peel and Leaf Extracts as Promising Strategies for HSV-1 Treatment |
title_full_unstemmed | Punica granatum Peel and Leaf Extracts as Promising Strategies for HSV-1 Treatment |
title_short | Punica granatum Peel and Leaf Extracts as Promising Strategies for HSV-1 Treatment |
title_sort | punica granatum peel and leaf extracts as promising strategies for hsv-1 treatment |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9782130/ https://www.ncbi.nlm.nih.gov/pubmed/36560643 http://dx.doi.org/10.3390/v14122639 |
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