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Insight into Different Stages of Steroid Degradation in Thermophilic Saccharopolyspora hirsuta VKM Ac-666(T) Strain

Steroids are abundant molecules in nature, and various microorganisms evolved to utilize steroids. Thermophilic actinobacteria play an important role in such processes. However, very few thermophiles have so far been reported capable of degrading or modifying natural sterols. Recently, genes putativ...

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Autores principales: Lobastova, Tatyana, Fokina, Victoria, Pozdnyakova-Filatova, Irina, Tarlachkov, Sergey, Shutov, Andrey, Donova, Marina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9782250/
https://www.ncbi.nlm.nih.gov/pubmed/36555813
http://dx.doi.org/10.3390/ijms232416174
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author Lobastova, Tatyana
Fokina, Victoria
Pozdnyakova-Filatova, Irina
Tarlachkov, Sergey
Shutov, Andrey
Donova, Marina
author_facet Lobastova, Tatyana
Fokina, Victoria
Pozdnyakova-Filatova, Irina
Tarlachkov, Sergey
Shutov, Andrey
Donova, Marina
author_sort Lobastova, Tatyana
collection PubMed
description Steroids are abundant molecules in nature, and various microorganisms evolved to utilize steroids. Thermophilic actinobacteria play an important role in such processes. However, very few thermophiles have so far been reported capable of degrading or modifying natural sterols. Recently, genes putatively involved in the sterol catabolic pathway have been revealed in the moderately thermophilic actinobacterium Saccharopolyspora hirsuta VKM Ac-666(T), but peculiarities of strain activity toward sterols are still poorly understood. S. hirsuta catalyzed cholesterol bioconversion at a rate significantly inferior to that observed for mesophilic actinobacteria (mycobacteria and rhodococci). Several genes related to different stages of steroid catabolism increased their expression in response to cholesterol as was shown by transcriptomic studies and verified by RT–qPCR. Sequential activation of genes related to the initial step of cholesterol side chain oxidation (cyp125) and later steps of steroid core degradation (kstD3, kshA, ipdF, and fadE30) was demonstrated for the first time. The activation correlates with a low cholesterol conversion rate and intermediate accumulation by the strain. The transcriptomic analyses revealed that the genes involved in sterol catabolism are linked functionally, but not transcriptionally. The results contribute to the knowledge on steroid catabolism in thermophilic actinobacteria and could be used at the engineering of microbial catalysts.
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spelling pubmed-97822502022-12-24 Insight into Different Stages of Steroid Degradation in Thermophilic Saccharopolyspora hirsuta VKM Ac-666(T) Strain Lobastova, Tatyana Fokina, Victoria Pozdnyakova-Filatova, Irina Tarlachkov, Sergey Shutov, Andrey Donova, Marina Int J Mol Sci Article Steroids are abundant molecules in nature, and various microorganisms evolved to utilize steroids. Thermophilic actinobacteria play an important role in such processes. However, very few thermophiles have so far been reported capable of degrading or modifying natural sterols. Recently, genes putatively involved in the sterol catabolic pathway have been revealed in the moderately thermophilic actinobacterium Saccharopolyspora hirsuta VKM Ac-666(T), but peculiarities of strain activity toward sterols are still poorly understood. S. hirsuta catalyzed cholesterol bioconversion at a rate significantly inferior to that observed for mesophilic actinobacteria (mycobacteria and rhodococci). Several genes related to different stages of steroid catabolism increased their expression in response to cholesterol as was shown by transcriptomic studies and verified by RT–qPCR. Sequential activation of genes related to the initial step of cholesterol side chain oxidation (cyp125) and later steps of steroid core degradation (kstD3, kshA, ipdF, and fadE30) was demonstrated for the first time. The activation correlates with a low cholesterol conversion rate and intermediate accumulation by the strain. The transcriptomic analyses revealed that the genes involved in sterol catabolism are linked functionally, but not transcriptionally. The results contribute to the knowledge on steroid catabolism in thermophilic actinobacteria and could be used at the engineering of microbial catalysts. MDPI 2022-12-18 /pmc/articles/PMC9782250/ /pubmed/36555813 http://dx.doi.org/10.3390/ijms232416174 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lobastova, Tatyana
Fokina, Victoria
Pozdnyakova-Filatova, Irina
Tarlachkov, Sergey
Shutov, Andrey
Donova, Marina
Insight into Different Stages of Steroid Degradation in Thermophilic Saccharopolyspora hirsuta VKM Ac-666(T) Strain
title Insight into Different Stages of Steroid Degradation in Thermophilic Saccharopolyspora hirsuta VKM Ac-666(T) Strain
title_full Insight into Different Stages of Steroid Degradation in Thermophilic Saccharopolyspora hirsuta VKM Ac-666(T) Strain
title_fullStr Insight into Different Stages of Steroid Degradation in Thermophilic Saccharopolyspora hirsuta VKM Ac-666(T) Strain
title_full_unstemmed Insight into Different Stages of Steroid Degradation in Thermophilic Saccharopolyspora hirsuta VKM Ac-666(T) Strain
title_short Insight into Different Stages of Steroid Degradation in Thermophilic Saccharopolyspora hirsuta VKM Ac-666(T) Strain
title_sort insight into different stages of steroid degradation in thermophilic saccharopolyspora hirsuta vkm ac-666(t) strain
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9782250/
https://www.ncbi.nlm.nih.gov/pubmed/36555813
http://dx.doi.org/10.3390/ijms232416174
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