Gene Editing Profiles in 94 CRISPR-Cas9 Expressing T(0) Transgenic Tobacco Lines Reveal High Frequencies of Chimeric Editing of the Target Gene

Chimeric editing is often reported in gene editing. To assess how the general chimeric editing is, we created a transgenic tobacco line carrying a marker, beta-glucuronidase gene (gusA), introduced a CRISPR-Cas9 editing vector into the transgenic tobacco line for knocking out gusA, and then investig...

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Detalles Bibliográficos
Autores principales: Song, Guo-Qing, Urban, Grace, Ryner, John T., Zhong, Gan-Yuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9782292/
https://www.ncbi.nlm.nih.gov/pubmed/36559603
http://dx.doi.org/10.3390/plants11243494

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9782292/
https://www.ncbi.nlm.nih.gov/pubmed/36559603
http://dx.doi.org/10.3390/plants11243494

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