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Gene Editing Profiles in 94 CRISPR-Cas9 Expressing T(0) Transgenic Tobacco Lines Reveal High Frequencies of Chimeric Editing of the Target Gene
Chimeric editing is often reported in gene editing. To assess how the general chimeric editing is, we created a transgenic tobacco line carrying a marker, beta-glucuronidase gene (gusA), introduced a CRISPR-Cas9 editing vector into the transgenic tobacco line for knocking out gusA, and then investig...
Autores principales: | Song, Guo-Qing, Urban, Grace, Ryner, John T., Zhong, Gan-Yuan |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9782292/ https://www.ncbi.nlm.nih.gov/pubmed/36559603 http://dx.doi.org/10.3390/plants11243494 |
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