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Immunoprotective Analysis of the NFA49590 Protein from Nocardia farcinica IFM 10152 Demonstrates Its Potential as a Vaccine Candidate

Nocardia is emerging as a serious and easily neglected pathogen in clinical practice with multidrug resistance that extends the treatment period for months or even years. This has led to the investigation of a vaccine approach to prevent Nocardia infections. However, studies on the protective protei...

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Autores principales: Han, Lichao, Ji, Xingzhao, Yang, Caixin, Zhao, Shuo, Fan, Shihong, Zhao, Lijun, Qiu, Xiaotong, Yao, Jiang, Liu, Xueping, Li, Fang, Li, Zhenjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9782307/
https://www.ncbi.nlm.nih.gov/pubmed/36558822
http://dx.doi.org/10.3390/pathogens11121488
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author Han, Lichao
Ji, Xingzhao
Yang, Caixin
Zhao, Shuo
Fan, Shihong
Zhao, Lijun
Qiu, Xiaotong
Yao, Jiang
Liu, Xueping
Li, Fang
Li, Zhenjun
author_facet Han, Lichao
Ji, Xingzhao
Yang, Caixin
Zhao, Shuo
Fan, Shihong
Zhao, Lijun
Qiu, Xiaotong
Yao, Jiang
Liu, Xueping
Li, Fang
Li, Zhenjun
author_sort Han, Lichao
collection PubMed
description Nocardia is emerging as a serious and easily neglected pathogen in clinical practice with multidrug resistance that extends the treatment period for months or even years. This has led to the investigation of a vaccine approach to prevent Nocardia infections. However, studies on the protective proteins of Nocardia have not yet been carried out. In the present work, over 500 proteins in the supernatant of N. farcinica IFM10152 were identified by LC–MS/MS. In silico analysis of these proteins with a high content (score > 2000) predicted that NFA49590 was one of the conserved proteins in N. farcinica strains with potential antigenicity. After the rNFA49590 protein was cloned and expressed in E. coli (DE3) and purified using a Ni-NTA column, its good antigenicity was confirmed with sera from mice immunized with different Nocardia species by Western blot. Then we confirmed its ability to activate innate immunity by examining the phosphorylation status of ERK1/2, JNK, p38, and p65 and the cytokine levels of IL-6, TNF-α, and IL-10. Finally, we evaluated its immunoprotective effect in BALB/c mice, and we found that mice immunized with rNFA49590 protein exhibited high antibody titers, enhanced bacterial clearance ability, and generated robust protective effects from the N. farcinica challenge. These results offer strong support for the use of NFA49590 protein as a vaccine candidate and open the possibilities for the exploration of a large array of immunoprotective proteins.
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spelling pubmed-97823072022-12-24 Immunoprotective Analysis of the NFA49590 Protein from Nocardia farcinica IFM 10152 Demonstrates Its Potential as a Vaccine Candidate Han, Lichao Ji, Xingzhao Yang, Caixin Zhao, Shuo Fan, Shihong Zhao, Lijun Qiu, Xiaotong Yao, Jiang Liu, Xueping Li, Fang Li, Zhenjun Pathogens Article Nocardia is emerging as a serious and easily neglected pathogen in clinical practice with multidrug resistance that extends the treatment period for months or even years. This has led to the investigation of a vaccine approach to prevent Nocardia infections. However, studies on the protective proteins of Nocardia have not yet been carried out. In the present work, over 500 proteins in the supernatant of N. farcinica IFM10152 were identified by LC–MS/MS. In silico analysis of these proteins with a high content (score > 2000) predicted that NFA49590 was one of the conserved proteins in N. farcinica strains with potential antigenicity. After the rNFA49590 protein was cloned and expressed in E. coli (DE3) and purified using a Ni-NTA column, its good antigenicity was confirmed with sera from mice immunized with different Nocardia species by Western blot. Then we confirmed its ability to activate innate immunity by examining the phosphorylation status of ERK1/2, JNK, p38, and p65 and the cytokine levels of IL-6, TNF-α, and IL-10. Finally, we evaluated its immunoprotective effect in BALB/c mice, and we found that mice immunized with rNFA49590 protein exhibited high antibody titers, enhanced bacterial clearance ability, and generated robust protective effects from the N. farcinica challenge. These results offer strong support for the use of NFA49590 protein as a vaccine candidate and open the possibilities for the exploration of a large array of immunoprotective proteins. MDPI 2022-12-07 /pmc/articles/PMC9782307/ /pubmed/36558822 http://dx.doi.org/10.3390/pathogens11121488 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Han, Lichao
Ji, Xingzhao
Yang, Caixin
Zhao, Shuo
Fan, Shihong
Zhao, Lijun
Qiu, Xiaotong
Yao, Jiang
Liu, Xueping
Li, Fang
Li, Zhenjun
Immunoprotective Analysis of the NFA49590 Protein from Nocardia farcinica IFM 10152 Demonstrates Its Potential as a Vaccine Candidate
title Immunoprotective Analysis of the NFA49590 Protein from Nocardia farcinica IFM 10152 Demonstrates Its Potential as a Vaccine Candidate
title_full Immunoprotective Analysis of the NFA49590 Protein from Nocardia farcinica IFM 10152 Demonstrates Its Potential as a Vaccine Candidate
title_fullStr Immunoprotective Analysis of the NFA49590 Protein from Nocardia farcinica IFM 10152 Demonstrates Its Potential as a Vaccine Candidate
title_full_unstemmed Immunoprotective Analysis of the NFA49590 Protein from Nocardia farcinica IFM 10152 Demonstrates Its Potential as a Vaccine Candidate
title_short Immunoprotective Analysis of the NFA49590 Protein from Nocardia farcinica IFM 10152 Demonstrates Its Potential as a Vaccine Candidate
title_sort immunoprotective analysis of the nfa49590 protein from nocardia farcinica ifm 10152 demonstrates its potential as a vaccine candidate
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9782307/
https://www.ncbi.nlm.nih.gov/pubmed/36558822
http://dx.doi.org/10.3390/pathogens11121488
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