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A Comparative Study of Three Approaches to Fibre’s Surface Functionalization

Polyester-based scaffolds are of research interest for the regeneration of a wide spectrum of tissues. However, there is a need to improve scaffold wettability and introduce bioactivity. Surface modification is a widely studied approach for improving scaffold performance and maintaining appropriate...

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Autores principales: Dulnik, Judyta, Jeznach, Oliwia, Sajkiewicz, Paweł
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9782664/
https://www.ncbi.nlm.nih.gov/pubmed/36547532
http://dx.doi.org/10.3390/jfb13040272
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author Dulnik, Judyta
Jeznach, Oliwia
Sajkiewicz, Paweł
author_facet Dulnik, Judyta
Jeznach, Oliwia
Sajkiewicz, Paweł
author_sort Dulnik, Judyta
collection PubMed
description Polyester-based scaffolds are of research interest for the regeneration of a wide spectrum of tissues. However, there is a need to improve scaffold wettability and introduce bioactivity. Surface modification is a widely studied approach for improving scaffold performance and maintaining appropriate bulk properties. In this study, three methods to functionalize the surface of the poly(lactide-co-ε-caprolactone) PLCL fibres using gelatin immobilisation were compared. Hydrolysis, oxygen plasma treatment, and aminolysis were chosen as activation methods to introduce carboxyl (-COOH) and amino (-NH(2)) functional groups on the surface before gelatin immobilisation. To covalently attach the gelatin, carbodiimide coupling was chosen for hydrolysed and plasma-treated materials, and glutaraldehyde crosslinking was used in the case of the aminolysed samples. Materials after physical entrapment of gelatin and immobilisation using carbodiimide coupling without previous activation were prepared as controls. The difference in gelatin amount on the surface, impact on the fibres morphology, molecular weight, and mechanical properties were observed depending on the type of modification and applied parameters of activation. It was shown that hydrolysis influences the surface of the material the most, whereas plasma treatment and aminolysis have an effect on the whole volume of the material. Despite this difference, bulk mechanical properties were affected for all the approaches. All materials were completely hydrophilic after functionalization. Cytotoxicity was not recognized for any of the samples. Gelatin immobilisation resulted in improved L929 cell morphology with the best effect for samples activated with hydrolysis and plasma treatment. Our study indicates that the use of any surface activation method should be limited to the lowest concentration/reaction time that enables subsequent satisfactory functionalization and the decision should be based on a specific function that the final scaffold material has to perform.
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spelling pubmed-97826642022-12-24 A Comparative Study of Three Approaches to Fibre’s Surface Functionalization Dulnik, Judyta Jeznach, Oliwia Sajkiewicz, Paweł J Funct Biomater Article Polyester-based scaffolds are of research interest for the regeneration of a wide spectrum of tissues. However, there is a need to improve scaffold wettability and introduce bioactivity. Surface modification is a widely studied approach for improving scaffold performance and maintaining appropriate bulk properties. In this study, three methods to functionalize the surface of the poly(lactide-co-ε-caprolactone) PLCL fibres using gelatin immobilisation were compared. Hydrolysis, oxygen plasma treatment, and aminolysis were chosen as activation methods to introduce carboxyl (-COOH) and amino (-NH(2)) functional groups on the surface before gelatin immobilisation. To covalently attach the gelatin, carbodiimide coupling was chosen for hydrolysed and plasma-treated materials, and glutaraldehyde crosslinking was used in the case of the aminolysed samples. Materials after physical entrapment of gelatin and immobilisation using carbodiimide coupling without previous activation were prepared as controls. The difference in gelatin amount on the surface, impact on the fibres morphology, molecular weight, and mechanical properties were observed depending on the type of modification and applied parameters of activation. It was shown that hydrolysis influences the surface of the material the most, whereas plasma treatment and aminolysis have an effect on the whole volume of the material. Despite this difference, bulk mechanical properties were affected for all the approaches. All materials were completely hydrophilic after functionalization. Cytotoxicity was not recognized for any of the samples. Gelatin immobilisation resulted in improved L929 cell morphology with the best effect for samples activated with hydrolysis and plasma treatment. Our study indicates that the use of any surface activation method should be limited to the lowest concentration/reaction time that enables subsequent satisfactory functionalization and the decision should be based on a specific function that the final scaffold material has to perform. MDPI 2022-12-02 /pmc/articles/PMC9782664/ /pubmed/36547532 http://dx.doi.org/10.3390/jfb13040272 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dulnik, Judyta
Jeznach, Oliwia
Sajkiewicz, Paweł
A Comparative Study of Three Approaches to Fibre’s Surface Functionalization
title A Comparative Study of Three Approaches to Fibre’s Surface Functionalization
title_full A Comparative Study of Three Approaches to Fibre’s Surface Functionalization
title_fullStr A Comparative Study of Three Approaches to Fibre’s Surface Functionalization
title_full_unstemmed A Comparative Study of Three Approaches to Fibre’s Surface Functionalization
title_short A Comparative Study of Three Approaches to Fibre’s Surface Functionalization
title_sort comparative study of three approaches to fibre’s surface functionalization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9782664/
https://www.ncbi.nlm.nih.gov/pubmed/36547532
http://dx.doi.org/10.3390/jfb13040272
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