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A dark intermediate in the fluorogenic reaction between tetrazine fluorophores and trans-cyclooctene

Fluorogenic labeling via bioorthogonal tetrazine chemistry has proven to be highly successful in fluorescence microscopy of living cells. To date, trans-cyclooctene (TCO) and bicyclonyne have been found to be the most useful substrates for live-cell labeling owing to their fast labeling kinetics, hi...

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Detalles Bibliográficos
Autores principales: Hild, Felix, Werther, Philipp, Yserentant, Klaus, Wombacher, Richard, Herten, Dirk-Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9782730/
https://www.ncbi.nlm.nih.gov/pubmed/36570717
http://dx.doi.org/10.1016/j.bpr.2022.100084