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A dark intermediate in the fluorogenic reaction between tetrazine fluorophores and trans-cyclooctene
Fluorogenic labeling via bioorthogonal tetrazine chemistry has proven to be highly successful in fluorescence microscopy of living cells. To date, trans-cyclooctene (TCO) and bicyclonyne have been found to be the most useful substrates for live-cell labeling owing to their fast labeling kinetics, hi...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9782730/ https://www.ncbi.nlm.nih.gov/pubmed/36570717 http://dx.doi.org/10.1016/j.bpr.2022.100084 |