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Detection and Molecular Characterization of Canine Distemper Virus in Wildlife from Northern Italy

Canine distemper virus (CDV) is a fatal, highly contagious disease found in wild and domestic carnivores. Several outbreaks have occurred in wildlife in Italy in recent years. This study aims to detect CDV in wildlife following the increasing mortality of foxes (Vulpes vulpes) in the Emilia-Romagna...

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Autores principales: Trogu, Tiziana, Castelli, Anna, Canziani, Sabrina, Tolini, Clara, Carrera, Maya, Sozzi, Enrica, Lelli, Davide, Tosi, Giovanni, Fiorentini, Laura, Di Donato, Alessandra, Rugna, Gianluca, Lanci, Danilo, Lavazza, Antonio, Moreno, Ana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9782791/
https://www.ncbi.nlm.nih.gov/pubmed/36558891
http://dx.doi.org/10.3390/pathogens11121557
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author Trogu, Tiziana
Castelli, Anna
Canziani, Sabrina
Tolini, Clara
Carrera, Maya
Sozzi, Enrica
Lelli, Davide
Tosi, Giovanni
Fiorentini, Laura
Di Donato, Alessandra
Rugna, Gianluca
Lanci, Danilo
Lavazza, Antonio
Moreno, Ana
author_facet Trogu, Tiziana
Castelli, Anna
Canziani, Sabrina
Tolini, Clara
Carrera, Maya
Sozzi, Enrica
Lelli, Davide
Tosi, Giovanni
Fiorentini, Laura
Di Donato, Alessandra
Rugna, Gianluca
Lanci, Danilo
Lavazza, Antonio
Moreno, Ana
author_sort Trogu, Tiziana
collection PubMed
description Canine distemper virus (CDV) is a fatal, highly contagious disease found in wild and domestic carnivores. Several outbreaks have occurred in wildlife in Italy in recent years. This study aims to detect CDV in wildlife following the increasing mortality of foxes (Vulpes vulpes) in the Emilia-Romagna region (northern Italy) observed in 2021. Sixty-seven foxes and one badger (Meles meles) were subjected to necropsy followed by histological examination and were analyzed with molecular techniques to detect the presence of CDV. Of the tested animals, 16% (nine foxes and one badger) were positive for CDV. Phylogenetic analysis showed two different lineages based on complete H gene sequences. The Europe/South America-1 lineage was detected in one fox from Modena, which resembled the CDV variant associated with a previous outbreak in northern Italy in 2018, while the European Wildlife lineage was detected in animals from the Rimini province. Amino acid analysis highlighted a Y549H mutation in all sequences collected, which is commonly associated with increased virulence.
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spelling pubmed-97827912022-12-24 Detection and Molecular Characterization of Canine Distemper Virus in Wildlife from Northern Italy Trogu, Tiziana Castelli, Anna Canziani, Sabrina Tolini, Clara Carrera, Maya Sozzi, Enrica Lelli, Davide Tosi, Giovanni Fiorentini, Laura Di Donato, Alessandra Rugna, Gianluca Lanci, Danilo Lavazza, Antonio Moreno, Ana Pathogens Article Canine distemper virus (CDV) is a fatal, highly contagious disease found in wild and domestic carnivores. Several outbreaks have occurred in wildlife in Italy in recent years. This study aims to detect CDV in wildlife following the increasing mortality of foxes (Vulpes vulpes) in the Emilia-Romagna region (northern Italy) observed in 2021. Sixty-seven foxes and one badger (Meles meles) were subjected to necropsy followed by histological examination and were analyzed with molecular techniques to detect the presence of CDV. Of the tested animals, 16% (nine foxes and one badger) were positive for CDV. Phylogenetic analysis showed two different lineages based on complete H gene sequences. The Europe/South America-1 lineage was detected in one fox from Modena, which resembled the CDV variant associated with a previous outbreak in northern Italy in 2018, while the European Wildlife lineage was detected in animals from the Rimini province. Amino acid analysis highlighted a Y549H mutation in all sequences collected, which is commonly associated with increased virulence. MDPI 2022-12-19 /pmc/articles/PMC9782791/ /pubmed/36558891 http://dx.doi.org/10.3390/pathogens11121557 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Trogu, Tiziana
Castelli, Anna
Canziani, Sabrina
Tolini, Clara
Carrera, Maya
Sozzi, Enrica
Lelli, Davide
Tosi, Giovanni
Fiorentini, Laura
Di Donato, Alessandra
Rugna, Gianluca
Lanci, Danilo
Lavazza, Antonio
Moreno, Ana
Detection and Molecular Characterization of Canine Distemper Virus in Wildlife from Northern Italy
title Detection and Molecular Characterization of Canine Distemper Virus in Wildlife from Northern Italy
title_full Detection and Molecular Characterization of Canine Distemper Virus in Wildlife from Northern Italy
title_fullStr Detection and Molecular Characterization of Canine Distemper Virus in Wildlife from Northern Italy
title_full_unstemmed Detection and Molecular Characterization of Canine Distemper Virus in Wildlife from Northern Italy
title_short Detection and Molecular Characterization of Canine Distemper Virus in Wildlife from Northern Italy
title_sort detection and molecular characterization of canine distemper virus in wildlife from northern italy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9782791/
https://www.ncbi.nlm.nih.gov/pubmed/36558891
http://dx.doi.org/10.3390/pathogens11121557
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