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Effect of Photodynamic Therapy with Chlorin e6 on Canine Tumors
This work aims to prepare pure Chlorin e6 (Ce6) and establish Ce6-mediated photodynamic therapy (Ce6-PDT) as a better therapy option for canine tumors as well as mouse tumor models. Five dogs suffering from various cancers were treated with Ce6-PDT from one to several times. After receiving the Ce6...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9782963/ https://www.ncbi.nlm.nih.gov/pubmed/36556469 http://dx.doi.org/10.3390/life12122102 |
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author | Shrestha, Rajeev Lee, Hyun Ji Lim, Junmo Gurung, Pallavi Thapa Magar, Til Bahadur Kim, Young-Tak Lee, Kija Bae, Seulgi Kim, Yong-Wan |
author_facet | Shrestha, Rajeev Lee, Hyun Ji Lim, Junmo Gurung, Pallavi Thapa Magar, Til Bahadur Kim, Young-Tak Lee, Kija Bae, Seulgi Kim, Yong-Wan |
author_sort | Shrestha, Rajeev |
collection | PubMed |
description | This work aims to prepare pure Chlorin e6 (Ce6) and establish Ce6-mediated photodynamic therapy (Ce6-PDT) as a better therapy option for canine tumors as well as mouse tumor models. Five dogs suffering from various cancers were treated with Ce6-PDT from one to several times. After receiving the Ce6 (2.5 mg/kg) for 3 h, tumors were illuminated superficially or interstitially with 660 nm light. Two dogs underwent Ce6-guided fluorescence imaging by photodynamic diagnosis (PDD). Cell proliferation and apoptosis were detected by the 4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and western blot assay, respectively. Ce6-PDT efficacy was also determined using melanoma and pancreatic cancer mouse models. Two veterinary patients with mammary carcinoma and histiocytic sarcoma had their tumors significantly diminished and showed improved health after receiving Ce6-PDT. Moreover, in the cases of canine tumors, the adjunctive use of Ce6-PDD revealed cancers that were not visible with white light viewing and provided a visual contrast from surrounding tissues. Also, in vivo, Ce6-PDT remarkably reduced melanoma and pancreatic tumors in the mouse model. These findings could pave the way for a better understanding of the underlying processes of Ce6-PDT, making it an effective and safe candidate for use in human and veterinary applications to abolish various cancers. |
format | Online Article Text |
id | pubmed-9782963 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97829632022-12-24 Effect of Photodynamic Therapy with Chlorin e6 on Canine Tumors Shrestha, Rajeev Lee, Hyun Ji Lim, Junmo Gurung, Pallavi Thapa Magar, Til Bahadur Kim, Young-Tak Lee, Kija Bae, Seulgi Kim, Yong-Wan Life (Basel) Article This work aims to prepare pure Chlorin e6 (Ce6) and establish Ce6-mediated photodynamic therapy (Ce6-PDT) as a better therapy option for canine tumors as well as mouse tumor models. Five dogs suffering from various cancers were treated with Ce6-PDT from one to several times. After receiving the Ce6 (2.5 mg/kg) for 3 h, tumors were illuminated superficially or interstitially with 660 nm light. Two dogs underwent Ce6-guided fluorescence imaging by photodynamic diagnosis (PDD). Cell proliferation and apoptosis were detected by the 4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and western blot assay, respectively. Ce6-PDT efficacy was also determined using melanoma and pancreatic cancer mouse models. Two veterinary patients with mammary carcinoma and histiocytic sarcoma had their tumors significantly diminished and showed improved health after receiving Ce6-PDT. Moreover, in the cases of canine tumors, the adjunctive use of Ce6-PDD revealed cancers that were not visible with white light viewing and provided a visual contrast from surrounding tissues. Also, in vivo, Ce6-PDT remarkably reduced melanoma and pancreatic tumors in the mouse model. These findings could pave the way for a better understanding of the underlying processes of Ce6-PDT, making it an effective and safe candidate for use in human and veterinary applications to abolish various cancers. MDPI 2022-12-14 /pmc/articles/PMC9782963/ /pubmed/36556469 http://dx.doi.org/10.3390/life12122102 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Shrestha, Rajeev Lee, Hyun Ji Lim, Junmo Gurung, Pallavi Thapa Magar, Til Bahadur Kim, Young-Tak Lee, Kija Bae, Seulgi Kim, Yong-Wan Effect of Photodynamic Therapy with Chlorin e6 on Canine Tumors |
title | Effect of Photodynamic Therapy with Chlorin e6 on Canine Tumors |
title_full | Effect of Photodynamic Therapy with Chlorin e6 on Canine Tumors |
title_fullStr | Effect of Photodynamic Therapy with Chlorin e6 on Canine Tumors |
title_full_unstemmed | Effect of Photodynamic Therapy with Chlorin e6 on Canine Tumors |
title_short | Effect of Photodynamic Therapy with Chlorin e6 on Canine Tumors |
title_sort | effect of photodynamic therapy with chlorin e6 on canine tumors |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9782963/ https://www.ncbi.nlm.nih.gov/pubmed/36556469 http://dx.doi.org/10.3390/life12122102 |
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