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Complexation of Oligo- and Polynucleotides with Methoxyphenyl-Functionalized Imidazolium Surfactants

Interaction between cationic surfactants and nucleic acids attracts much attention due to the possibility of using such systems for gene delivery. Herein, the lipoplexes based on cationic surfactants with imidazolium head group bearing methoxyphenyl fragment (MPI-n, n = 10, 12, 14, 16) and nucleic a...

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Autores principales: Kuznetsova, Darya A., Kuznetsov, Denis M., Vasileva, Leysan A., Amerhanova, Syumbelya K., Valeeva, Dilyara N., Salakhieva, Diana V., Nikolaeva, Viktoriia A., Nizameev, Irek R., Islamov, Daut R., Usachev, Konstantin S., Voloshina, Alexandra D., Zakharova, Lucia Ya.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9782993/
https://www.ncbi.nlm.nih.gov/pubmed/36559178
http://dx.doi.org/10.3390/pharmaceutics14122685
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author Kuznetsova, Darya A.
Kuznetsov, Denis M.
Vasileva, Leysan A.
Amerhanova, Syumbelya K.
Valeeva, Dilyara N.
Salakhieva, Diana V.
Nikolaeva, Viktoriia A.
Nizameev, Irek R.
Islamov, Daut R.
Usachev, Konstantin S.
Voloshina, Alexandra D.
Zakharova, Lucia Ya.
author_facet Kuznetsova, Darya A.
Kuznetsov, Denis M.
Vasileva, Leysan A.
Amerhanova, Syumbelya K.
Valeeva, Dilyara N.
Salakhieva, Diana V.
Nikolaeva, Viktoriia A.
Nizameev, Irek R.
Islamov, Daut R.
Usachev, Konstantin S.
Voloshina, Alexandra D.
Zakharova, Lucia Ya.
author_sort Kuznetsova, Darya A.
collection PubMed
description Interaction between cationic surfactants and nucleic acids attracts much attention due to the possibility of using such systems for gene delivery. Herein, the lipoplexes based on cationic surfactants with imidazolium head group bearing methoxyphenyl fragment (MPI-n, n = 10, 12, 14, 16) and nucleic acids (oligonucleotide and plasmid DNA) were explored. The complex formation was confirmed by dynamic/electrophoretic light scattering, transmission electron microscopy, fluorescence spectroscopy, circular dichroism, and gel electrophoresis. The nanosized lipoplex formation (of about 100–200 nm), contributed by electrostatic, hydrophobic interactions, and intercalation mechanism, has been shown. Significant effects of the hydrocarbon tail length of surfactant and the type of nucleic acid on their interaction was revealed. The cytotoxic effect and transfection ability of lipoplexes studied were determined using M-HeLa, A549 cancer cell lines, and normal Chang liver cells. A selective reduced cytotoxic effect of the complexes on M-HeLa cancer cells was established, as well as a high ability of the systems to be transfected into cancer cells. MPI-n/DNA complexes showed a pronounced transfection activity equal to the commercial preparation Lipofectamine 3000. Thus, it has been shown that MPI-n surfactants are effective agents for nucleic acid condensation and can be considered as potential non-viral vectors for gene delivery.
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spelling pubmed-97829932022-12-24 Complexation of Oligo- and Polynucleotides with Methoxyphenyl-Functionalized Imidazolium Surfactants Kuznetsova, Darya A. Kuznetsov, Denis M. Vasileva, Leysan A. Amerhanova, Syumbelya K. Valeeva, Dilyara N. Salakhieva, Diana V. Nikolaeva, Viktoriia A. Nizameev, Irek R. Islamov, Daut R. Usachev, Konstantin S. Voloshina, Alexandra D. Zakharova, Lucia Ya. Pharmaceutics Article Interaction between cationic surfactants and nucleic acids attracts much attention due to the possibility of using such systems for gene delivery. Herein, the lipoplexes based on cationic surfactants with imidazolium head group bearing methoxyphenyl fragment (MPI-n, n = 10, 12, 14, 16) and nucleic acids (oligonucleotide and plasmid DNA) were explored. The complex formation was confirmed by dynamic/electrophoretic light scattering, transmission electron microscopy, fluorescence spectroscopy, circular dichroism, and gel electrophoresis. The nanosized lipoplex formation (of about 100–200 nm), contributed by electrostatic, hydrophobic interactions, and intercalation mechanism, has been shown. Significant effects of the hydrocarbon tail length of surfactant and the type of nucleic acid on their interaction was revealed. The cytotoxic effect and transfection ability of lipoplexes studied were determined using M-HeLa, A549 cancer cell lines, and normal Chang liver cells. A selective reduced cytotoxic effect of the complexes on M-HeLa cancer cells was established, as well as a high ability of the systems to be transfected into cancer cells. MPI-n/DNA complexes showed a pronounced transfection activity equal to the commercial preparation Lipofectamine 3000. Thus, it has been shown that MPI-n surfactants are effective agents for nucleic acid condensation and can be considered as potential non-viral vectors for gene delivery. MDPI 2022-12-01 /pmc/articles/PMC9782993/ /pubmed/36559178 http://dx.doi.org/10.3390/pharmaceutics14122685 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kuznetsova, Darya A.
Kuznetsov, Denis M.
Vasileva, Leysan A.
Amerhanova, Syumbelya K.
Valeeva, Dilyara N.
Salakhieva, Diana V.
Nikolaeva, Viktoriia A.
Nizameev, Irek R.
Islamov, Daut R.
Usachev, Konstantin S.
Voloshina, Alexandra D.
Zakharova, Lucia Ya.
Complexation of Oligo- and Polynucleotides with Methoxyphenyl-Functionalized Imidazolium Surfactants
title Complexation of Oligo- and Polynucleotides with Methoxyphenyl-Functionalized Imidazolium Surfactants
title_full Complexation of Oligo- and Polynucleotides with Methoxyphenyl-Functionalized Imidazolium Surfactants
title_fullStr Complexation of Oligo- and Polynucleotides with Methoxyphenyl-Functionalized Imidazolium Surfactants
title_full_unstemmed Complexation of Oligo- and Polynucleotides with Methoxyphenyl-Functionalized Imidazolium Surfactants
title_short Complexation of Oligo- and Polynucleotides with Methoxyphenyl-Functionalized Imidazolium Surfactants
title_sort complexation of oligo- and polynucleotides with methoxyphenyl-functionalized imidazolium surfactants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9782993/
https://www.ncbi.nlm.nih.gov/pubmed/36559178
http://dx.doi.org/10.3390/pharmaceutics14122685
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