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Does Melatonin Associated with Nanostructured Calcium Phosphate Improve Alveolar Bone Repair?

Background and objectives: Calcium phosphates have been widely used as bone substitutes, but their properties are limited to osteoconduction. The association of calcium phosphates with osteoinductive bioactive molecules has been used as a strategy in regenerative medicine. Melatonin has been studied...

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Autores principales: de Almeida, Camila Diuana, Sartoretto, Suelen Cristina, Alves, Adriana Terezinha Neves Novellino, de Brito Resende, Rodrigo Figueiredo, de Albuquerque Calasans-Maia, Jose, Moraschini, Vittorio, Rossi, Alexandre, Granjeiro, José Mauro, Sacco, Roberto, Calasans-Maia, Mônica Diuana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9783045/
https://www.ncbi.nlm.nih.gov/pubmed/36556923
http://dx.doi.org/10.3390/medicina58121720
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author de Almeida, Camila Diuana
Sartoretto, Suelen Cristina
Alves, Adriana Terezinha Neves Novellino
de Brito Resende, Rodrigo Figueiredo
de Albuquerque Calasans-Maia, Jose
Moraschini, Vittorio
Rossi, Alexandre
Granjeiro, José Mauro
Sacco, Roberto
Calasans-Maia, Mônica Diuana
author_facet de Almeida, Camila Diuana
Sartoretto, Suelen Cristina
Alves, Adriana Terezinha Neves Novellino
de Brito Resende, Rodrigo Figueiredo
de Albuquerque Calasans-Maia, Jose
Moraschini, Vittorio
Rossi, Alexandre
Granjeiro, José Mauro
Sacco, Roberto
Calasans-Maia, Mônica Diuana
author_sort de Almeida, Camila Diuana
collection PubMed
description Background and objectives: Calcium phosphates have been widely used as bone substitutes, but their properties are limited to osteoconduction. The association of calcium phosphates with osteoinductive bioactive molecules has been used as a strategy in regenerative medicine. Melatonin has been studied due to its cell protection and antioxidant functions, reducing osteoclastic activity and stimulating newly formed bone. This study aimed to evaluate the effect of topical application of melatonin associated with nanostructured carbonated hydroxyapatite microspheres in the alveolar bone repair of Wistar rats through histological and histomorphometric analysis. Materials and Methods: Thirty female Wistar rats (300 g) were used, divided randomly into three experimental groups (n = 10), G1: nanostructured carbonated hydroxyapatite microspheres associated with melatonin gel (CHA-M); G2: nanostructured carbonated hydroxyapatite (CHA); G3: blood clot (without alveolar filling). The animals were euthanized after 7 and 42 days of the postoperative period and processed for histological and histomorphometric evaluation. Kruskal–Wallis and Dunn’s post-test were applied to investigate statistical differences between the groups at the same time point for new bone and connective tissue variables. Mann–Whitney was used to assess statistical differences between different time points and in the biomaterial variable. Results: Results showed a greater volume of residual biomaterial in the CHA-M than the CHA group (p = 0.007), and there were no significant differences in terms of newly formed bone and connective tissue between CHA and CHA-M after 42 days. Conclusions: This study concluded that both biomaterials improved alveolar bone repair from 7 to 42 days after surgery, and the association of CHA with melatonin gel reduced the biomaterial’s biodegradation at the implanted site but did not improve the alveolar bone repair.
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spelling pubmed-97830452022-12-24 Does Melatonin Associated with Nanostructured Calcium Phosphate Improve Alveolar Bone Repair? de Almeida, Camila Diuana Sartoretto, Suelen Cristina Alves, Adriana Terezinha Neves Novellino de Brito Resende, Rodrigo Figueiredo de Albuquerque Calasans-Maia, Jose Moraschini, Vittorio Rossi, Alexandre Granjeiro, José Mauro Sacco, Roberto Calasans-Maia, Mônica Diuana Medicina (Kaunas) Article Background and objectives: Calcium phosphates have been widely used as bone substitutes, but their properties are limited to osteoconduction. The association of calcium phosphates with osteoinductive bioactive molecules has been used as a strategy in regenerative medicine. Melatonin has been studied due to its cell protection and antioxidant functions, reducing osteoclastic activity and stimulating newly formed bone. This study aimed to evaluate the effect of topical application of melatonin associated with nanostructured carbonated hydroxyapatite microspheres in the alveolar bone repair of Wistar rats through histological and histomorphometric analysis. Materials and Methods: Thirty female Wistar rats (300 g) were used, divided randomly into three experimental groups (n = 10), G1: nanostructured carbonated hydroxyapatite microspheres associated with melatonin gel (CHA-M); G2: nanostructured carbonated hydroxyapatite (CHA); G3: blood clot (without alveolar filling). The animals were euthanized after 7 and 42 days of the postoperative period and processed for histological and histomorphometric evaluation. Kruskal–Wallis and Dunn’s post-test were applied to investigate statistical differences between the groups at the same time point for new bone and connective tissue variables. Mann–Whitney was used to assess statistical differences between different time points and in the biomaterial variable. Results: Results showed a greater volume of residual biomaterial in the CHA-M than the CHA group (p = 0.007), and there were no significant differences in terms of newly formed bone and connective tissue between CHA and CHA-M after 42 days. Conclusions: This study concluded that both biomaterials improved alveolar bone repair from 7 to 42 days after surgery, and the association of CHA with melatonin gel reduced the biomaterial’s biodegradation at the implanted site but did not improve the alveolar bone repair. MDPI 2022-11-24 /pmc/articles/PMC9783045/ /pubmed/36556923 http://dx.doi.org/10.3390/medicina58121720 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
de Almeida, Camila Diuana
Sartoretto, Suelen Cristina
Alves, Adriana Terezinha Neves Novellino
de Brito Resende, Rodrigo Figueiredo
de Albuquerque Calasans-Maia, Jose
Moraschini, Vittorio
Rossi, Alexandre
Granjeiro, José Mauro
Sacco, Roberto
Calasans-Maia, Mônica Diuana
Does Melatonin Associated with Nanostructured Calcium Phosphate Improve Alveolar Bone Repair?
title Does Melatonin Associated with Nanostructured Calcium Phosphate Improve Alveolar Bone Repair?
title_full Does Melatonin Associated with Nanostructured Calcium Phosphate Improve Alveolar Bone Repair?
title_fullStr Does Melatonin Associated with Nanostructured Calcium Phosphate Improve Alveolar Bone Repair?
title_full_unstemmed Does Melatonin Associated with Nanostructured Calcium Phosphate Improve Alveolar Bone Repair?
title_short Does Melatonin Associated with Nanostructured Calcium Phosphate Improve Alveolar Bone Repair?
title_sort does melatonin associated with nanostructured calcium phosphate improve alveolar bone repair?
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9783045/
https://www.ncbi.nlm.nih.gov/pubmed/36556923
http://dx.doi.org/10.3390/medicina58121720
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