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Expression of Cytokine Profiles in Human THP-1 Cells during Phase Transition of Talaromyces marneffei

Talaromyces marneffei, a dimorphic fungus, exhibits temperature-dependent growth, existing in a filamentous form at 25 °C and as a yeast at 37 °C. Several studies have highlighted the important roles of macrophages in defense against T. marneffei infection. However, the immune responses to the inter...

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Autores principales: Shu, Fangyi, Thammasit, Patcharin, Pruksaphon, Kritsada, Nosanchuk, Joshua D., Youngchim, Sirida
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9783046/
https://www.ncbi.nlm.nih.gov/pubmed/36558799
http://dx.doi.org/10.3390/pathogens11121465
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author Shu, Fangyi
Thammasit, Patcharin
Pruksaphon, Kritsada
Nosanchuk, Joshua D.
Youngchim, Sirida
author_facet Shu, Fangyi
Thammasit, Patcharin
Pruksaphon, Kritsada
Nosanchuk, Joshua D.
Youngchim, Sirida
author_sort Shu, Fangyi
collection PubMed
description Talaromyces marneffei, a dimorphic fungus, exhibits temperature-dependent growth, existing in a filamentous form at 25 °C and as a yeast at 37 °C. Several studies have highlighted the important roles of macrophages in defense against T. marneffei infection. However, the immune responses to the interaction of macrophages with T. marneffei cells during phase transition require further investigation. This study reports the expression of cytokine profiles in human THP-1 cells during infection by T. marneffei. THP-1 cells were infected with T. marneffei conidia at different multiplicity of infections (MOIs). Surviving conidia transformed into yeasts after phagocytosis by macrophages, and the number of yeasts gradually increased over 36 h. The transcription and secretion levels of pro- and anti-inflammatory cytokines were examined at different times by qRT-PCR and ELISA. Transcription levels of IL-8, IL-12, IL-1β, and TNF-α increased significantly at 12 or 24 h and then slightly decreased at 36 h. In contrast, the transcription levels of IL-6, IL-10, and TGF-β gradually increased at all MOIs. The levels of IL-6 and IL-10 secretion corresponded to their levels of transcription. These results indicated that as the number of intracellular yeasts increased, the infected macrophages first underwent slight M1 polarization before shifting to M2 polarization. This polarization transition was confirmed by the fungicidal ability and the expression of macrophage surface markers. By inducing the M2-type polarization of macrophages, the intracellular T. marneffei cells can successfully evade the immune response. Our study provides a novel insight into the immune characterization during the transition of T. marneffei infection and could further contribute to possible diagnostic and therapeutic interventions for this infection.
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spelling pubmed-97830462022-12-24 Expression of Cytokine Profiles in Human THP-1 Cells during Phase Transition of Talaromyces marneffei Shu, Fangyi Thammasit, Patcharin Pruksaphon, Kritsada Nosanchuk, Joshua D. Youngchim, Sirida Pathogens Article Talaromyces marneffei, a dimorphic fungus, exhibits temperature-dependent growth, existing in a filamentous form at 25 °C and as a yeast at 37 °C. Several studies have highlighted the important roles of macrophages in defense against T. marneffei infection. However, the immune responses to the interaction of macrophages with T. marneffei cells during phase transition require further investigation. This study reports the expression of cytokine profiles in human THP-1 cells during infection by T. marneffei. THP-1 cells were infected with T. marneffei conidia at different multiplicity of infections (MOIs). Surviving conidia transformed into yeasts after phagocytosis by macrophages, and the number of yeasts gradually increased over 36 h. The transcription and secretion levels of pro- and anti-inflammatory cytokines were examined at different times by qRT-PCR and ELISA. Transcription levels of IL-8, IL-12, IL-1β, and TNF-α increased significantly at 12 or 24 h and then slightly decreased at 36 h. In contrast, the transcription levels of IL-6, IL-10, and TGF-β gradually increased at all MOIs. The levels of IL-6 and IL-10 secretion corresponded to their levels of transcription. These results indicated that as the number of intracellular yeasts increased, the infected macrophages first underwent slight M1 polarization before shifting to M2 polarization. This polarization transition was confirmed by the fungicidal ability and the expression of macrophage surface markers. By inducing the M2-type polarization of macrophages, the intracellular T. marneffei cells can successfully evade the immune response. Our study provides a novel insight into the immune characterization during the transition of T. marneffei infection and could further contribute to possible diagnostic and therapeutic interventions for this infection. MDPI 2022-12-02 /pmc/articles/PMC9783046/ /pubmed/36558799 http://dx.doi.org/10.3390/pathogens11121465 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shu, Fangyi
Thammasit, Patcharin
Pruksaphon, Kritsada
Nosanchuk, Joshua D.
Youngchim, Sirida
Expression of Cytokine Profiles in Human THP-1 Cells during Phase Transition of Talaromyces marneffei
title Expression of Cytokine Profiles in Human THP-1 Cells during Phase Transition of Talaromyces marneffei
title_full Expression of Cytokine Profiles in Human THP-1 Cells during Phase Transition of Talaromyces marneffei
title_fullStr Expression of Cytokine Profiles in Human THP-1 Cells during Phase Transition of Talaromyces marneffei
title_full_unstemmed Expression of Cytokine Profiles in Human THP-1 Cells during Phase Transition of Talaromyces marneffei
title_short Expression of Cytokine Profiles in Human THP-1 Cells during Phase Transition of Talaromyces marneffei
title_sort expression of cytokine profiles in human thp-1 cells during phase transition of talaromyces marneffei
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9783046/
https://www.ncbi.nlm.nih.gov/pubmed/36558799
http://dx.doi.org/10.3390/pathogens11121465
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