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Tropism and Infectivity of Pandemic Influenza A H1N1/09 Virus in the Human Placenta

Influenza virus infection in pregnant women may put the fetus at higher risk; however, to date, there has been no detailed research about the expression of influenza virus receptors in the human placenta. We employed the lectin staining technique, which is a classic influenza virus receptor research...

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Autores principales: Xiao, Yan-Na, Yu, Fei-Yuan, Xu, Qian, Gu, Jiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9783296/
https://www.ncbi.nlm.nih.gov/pubmed/36560811
http://dx.doi.org/10.3390/v14122807
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author Xiao, Yan-Na
Yu, Fei-Yuan
Xu, Qian
Gu, Jiang
author_facet Xiao, Yan-Na
Yu, Fei-Yuan
Xu, Qian
Gu, Jiang
author_sort Xiao, Yan-Na
collection PubMed
description Influenza virus infection in pregnant women may put the fetus at higher risk; however, to date, there has been no detailed research about the expression of influenza virus receptors in the human placenta. We employed the lectin staining technique, which is a classic influenza virus receptor research method for studying the distribution of viral receptors in the human placenta. In addition, we examined the susceptibility of the human placenta to H1N1/09, by detecting viral proteins and RNA at different time points post-infection. We found that the human placenta expressed both avian and human influenza A virus receptors (α-2, 3-linked sialic acid and α-2, 6-linked sialic acid). In addition, H1N1/09 did not only infect the human placenta, but also replicated and was released into the culture media. We concluded that the human placenta is susceptible to the 2009 influenza A virus (H1N1/09) infection, and that particular attention should be paid to shielding pregnant women from infection during influenza season.
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spelling pubmed-97832962022-12-24 Tropism and Infectivity of Pandemic Influenza A H1N1/09 Virus in the Human Placenta Xiao, Yan-Na Yu, Fei-Yuan Xu, Qian Gu, Jiang Viruses Article Influenza virus infection in pregnant women may put the fetus at higher risk; however, to date, there has been no detailed research about the expression of influenza virus receptors in the human placenta. We employed the lectin staining technique, which is a classic influenza virus receptor research method for studying the distribution of viral receptors in the human placenta. In addition, we examined the susceptibility of the human placenta to H1N1/09, by detecting viral proteins and RNA at different time points post-infection. We found that the human placenta expressed both avian and human influenza A virus receptors (α-2, 3-linked sialic acid and α-2, 6-linked sialic acid). In addition, H1N1/09 did not only infect the human placenta, but also replicated and was released into the culture media. We concluded that the human placenta is susceptible to the 2009 influenza A virus (H1N1/09) infection, and that particular attention should be paid to shielding pregnant women from infection during influenza season. MDPI 2022-12-15 /pmc/articles/PMC9783296/ /pubmed/36560811 http://dx.doi.org/10.3390/v14122807 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xiao, Yan-Na
Yu, Fei-Yuan
Xu, Qian
Gu, Jiang
Tropism and Infectivity of Pandemic Influenza A H1N1/09 Virus in the Human Placenta
title Tropism and Infectivity of Pandemic Influenza A H1N1/09 Virus in the Human Placenta
title_full Tropism and Infectivity of Pandemic Influenza A H1N1/09 Virus in the Human Placenta
title_fullStr Tropism and Infectivity of Pandemic Influenza A H1N1/09 Virus in the Human Placenta
title_full_unstemmed Tropism and Infectivity of Pandemic Influenza A H1N1/09 Virus in the Human Placenta
title_short Tropism and Infectivity of Pandemic Influenza A H1N1/09 Virus in the Human Placenta
title_sort tropism and infectivity of pandemic influenza a h1n1/09 virus in the human placenta
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9783296/
https://www.ncbi.nlm.nih.gov/pubmed/36560811
http://dx.doi.org/10.3390/v14122807
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