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Study on the Effect of Relaying on Norovirus Reduction from Crassostrea gigas Oysters

Norovirus (NoV) is the most important cause of seafood-borne gastroenteritis worldwide, mainly associated with the consumption of raw oysters. NoV is often present in oysters that comply with existing control standards for shellfish. Therefore, the improvement of post-harvest treatments and practice...

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Autores principales: Battistini, Roberta, Masotti, Chiara, Maurella, Cristiana, Costa, Erica, Orlandi, Mino, Feletti, Mirvana, Ercolini, Carlo, Serracca, Laura
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9783373/
https://www.ncbi.nlm.nih.gov/pubmed/36557642
http://dx.doi.org/10.3390/microorganisms10122389
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author Battistini, Roberta
Masotti, Chiara
Maurella, Cristiana
Costa, Erica
Orlandi, Mino
Feletti, Mirvana
Ercolini, Carlo
Serracca, Laura
author_facet Battistini, Roberta
Masotti, Chiara
Maurella, Cristiana
Costa, Erica
Orlandi, Mino
Feletti, Mirvana
Ercolini, Carlo
Serracca, Laura
author_sort Battistini, Roberta
collection PubMed
description Norovirus (NoV) is the most important cause of seafood-borne gastroenteritis worldwide, mainly associated with the consumption of raw oysters. NoV is often present in oysters that comply with existing control standards for shellfish. Therefore, the improvement of post-harvest treatments and practices can represent one of the main strategies to reduce the incidence of viral diseases related to shellfish. This study aimed to investigate long-term relays for the reduction of NoV levels in live oysters, during the high-risk cold months, by transferring the oysters from a more contaminated site to two sites with lower NoV levels. The efficacy of relaying was evaluated by analyzing oyster samples collected at days 0 (T0) and 30 (T30) for NoV levels using a real-time quantitative PCR (RT-qPCR). The NoV level at the relay sites was consistently lower than at the harvest site. The relay process for 30 days in seawater with a lower NoV level resulted in a decrease in the NoV load compared to day 0 with significant reductions depending on the site and genogroup of NoV considered. These results suggest that long-term relaying of oysters to reduce NoV levels is promising and could help growers to improve oyster safety; however, further investigations are needed.
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spelling pubmed-97833732022-12-24 Study on the Effect of Relaying on Norovirus Reduction from Crassostrea gigas Oysters Battistini, Roberta Masotti, Chiara Maurella, Cristiana Costa, Erica Orlandi, Mino Feletti, Mirvana Ercolini, Carlo Serracca, Laura Microorganisms Communication Norovirus (NoV) is the most important cause of seafood-borne gastroenteritis worldwide, mainly associated with the consumption of raw oysters. NoV is often present in oysters that comply with existing control standards for shellfish. Therefore, the improvement of post-harvest treatments and practices can represent one of the main strategies to reduce the incidence of viral diseases related to shellfish. This study aimed to investigate long-term relays for the reduction of NoV levels in live oysters, during the high-risk cold months, by transferring the oysters from a more contaminated site to two sites with lower NoV levels. The efficacy of relaying was evaluated by analyzing oyster samples collected at days 0 (T0) and 30 (T30) for NoV levels using a real-time quantitative PCR (RT-qPCR). The NoV level at the relay sites was consistently lower than at the harvest site. The relay process for 30 days in seawater with a lower NoV level resulted in a decrease in the NoV load compared to day 0 with significant reductions depending on the site and genogroup of NoV considered. These results suggest that long-term relaying of oysters to reduce NoV levels is promising and could help growers to improve oyster safety; however, further investigations are needed. MDPI 2022-12-01 /pmc/articles/PMC9783373/ /pubmed/36557642 http://dx.doi.org/10.3390/microorganisms10122389 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Battistini, Roberta
Masotti, Chiara
Maurella, Cristiana
Costa, Erica
Orlandi, Mino
Feletti, Mirvana
Ercolini, Carlo
Serracca, Laura
Study on the Effect of Relaying on Norovirus Reduction from Crassostrea gigas Oysters
title Study on the Effect of Relaying on Norovirus Reduction from Crassostrea gigas Oysters
title_full Study on the Effect of Relaying on Norovirus Reduction from Crassostrea gigas Oysters
title_fullStr Study on the Effect of Relaying on Norovirus Reduction from Crassostrea gigas Oysters
title_full_unstemmed Study on the Effect of Relaying on Norovirus Reduction from Crassostrea gigas Oysters
title_short Study on the Effect of Relaying on Norovirus Reduction from Crassostrea gigas Oysters
title_sort study on the effect of relaying on norovirus reduction from crassostrea gigas oysters
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9783373/
https://www.ncbi.nlm.nih.gov/pubmed/36557642
http://dx.doi.org/10.3390/microorganisms10122389
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