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Targeted Metabolome Profiling of Indonesian Shallots and Japanese Long-Day/Short-Day Bulb Onions

In this study, targeted metabolome analysis was applied to identify the discriminative metabolites between Indonesian shallot landraces, Japanese long-day onion (LDO) varieties, and Japanese short-day onion (SDO) varieties. In total, 172 metabolite signal intensities were subjected to multivariate P...

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Autores principales: Matsuse, Kanako, Abdelrahman, Mostafa, Ariyanti, Nur Aeni, Tsuji, Fumitada, Hirata, Sho, Nakajima, Tetsuya, Sato, Muneo, Hirai, Masami Yokota, Manochai, Benya, Shigyo, Masayoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9783499/
https://www.ncbi.nlm.nih.gov/pubmed/36557300
http://dx.doi.org/10.3390/metabo12121260
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author Matsuse, Kanako
Abdelrahman, Mostafa
Ariyanti, Nur Aeni
Tsuji, Fumitada
Hirata, Sho
Nakajima, Tetsuya
Sato, Muneo
Hirai, Masami Yokota
Manochai, Benya
Shigyo, Masayoshi
author_facet Matsuse, Kanako
Abdelrahman, Mostafa
Ariyanti, Nur Aeni
Tsuji, Fumitada
Hirata, Sho
Nakajima, Tetsuya
Sato, Muneo
Hirai, Masami Yokota
Manochai, Benya
Shigyo, Masayoshi
author_sort Matsuse, Kanako
collection PubMed
description In this study, targeted metabolome analysis was applied to identify the discriminative metabolites between Indonesian shallot landraces, Japanese long-day onion (LDO) varieties, and Japanese short-day onion (SDO) varieties. In total, 172 metabolite signal intensities were subjected to multivariate PLS-DA, VIP, and random forest modeling to gain further insight into genotype-specific metabolites. PLS-DA divides the examined genotypes into three different clusters, implying that shallot landraces exhibited a distinct metabolite profile compared with Japanese LDO and SDO varieties. The PLS-DA, VIP, and random forest results indicated that the shallot and LDO are richer in metabolite constituents in comparison with the SDO. Specifically, amino acids and organosulfur compounds were the key characteristic metabolites in shallot and LDO genotypes. The analysis of S-alk(en)yl-L-cysteine sulfoxide (ACSO) compounds showed higher accumulation in the shallot landraces relative to LDO and SDO varieties, which explains the stronger pungency and odor in shallots. In addition, the LDO showed higher ACSO content compared with the SDO, implying that long-day cultivation might enhance sulfur assimilation in the Japanese onion. The LDO ‘Super Kitamomiji’ and the shallots ‘Probolinggo’ and ‘Thailand’ showed higher ACSO content than other varieties, making it useful for Allium breeding to improve the flavor and stress tolerance of onions.
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spelling pubmed-97834992022-12-24 Targeted Metabolome Profiling of Indonesian Shallots and Japanese Long-Day/Short-Day Bulb Onions Matsuse, Kanako Abdelrahman, Mostafa Ariyanti, Nur Aeni Tsuji, Fumitada Hirata, Sho Nakajima, Tetsuya Sato, Muneo Hirai, Masami Yokota Manochai, Benya Shigyo, Masayoshi Metabolites Article In this study, targeted metabolome analysis was applied to identify the discriminative metabolites between Indonesian shallot landraces, Japanese long-day onion (LDO) varieties, and Japanese short-day onion (SDO) varieties. In total, 172 metabolite signal intensities were subjected to multivariate PLS-DA, VIP, and random forest modeling to gain further insight into genotype-specific metabolites. PLS-DA divides the examined genotypes into three different clusters, implying that shallot landraces exhibited a distinct metabolite profile compared with Japanese LDO and SDO varieties. The PLS-DA, VIP, and random forest results indicated that the shallot and LDO are richer in metabolite constituents in comparison with the SDO. Specifically, amino acids and organosulfur compounds were the key characteristic metabolites in shallot and LDO genotypes. The analysis of S-alk(en)yl-L-cysteine sulfoxide (ACSO) compounds showed higher accumulation in the shallot landraces relative to LDO and SDO varieties, which explains the stronger pungency and odor in shallots. In addition, the LDO showed higher ACSO content compared with the SDO, implying that long-day cultivation might enhance sulfur assimilation in the Japanese onion. The LDO ‘Super Kitamomiji’ and the shallots ‘Probolinggo’ and ‘Thailand’ showed higher ACSO content than other varieties, making it useful for Allium breeding to improve the flavor and stress tolerance of onions. MDPI 2022-12-14 /pmc/articles/PMC9783499/ /pubmed/36557300 http://dx.doi.org/10.3390/metabo12121260 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Matsuse, Kanako
Abdelrahman, Mostafa
Ariyanti, Nur Aeni
Tsuji, Fumitada
Hirata, Sho
Nakajima, Tetsuya
Sato, Muneo
Hirai, Masami Yokota
Manochai, Benya
Shigyo, Masayoshi
Targeted Metabolome Profiling of Indonesian Shallots and Japanese Long-Day/Short-Day Bulb Onions
title Targeted Metabolome Profiling of Indonesian Shallots and Japanese Long-Day/Short-Day Bulb Onions
title_full Targeted Metabolome Profiling of Indonesian Shallots and Japanese Long-Day/Short-Day Bulb Onions
title_fullStr Targeted Metabolome Profiling of Indonesian Shallots and Japanese Long-Day/Short-Day Bulb Onions
title_full_unstemmed Targeted Metabolome Profiling of Indonesian Shallots and Japanese Long-Day/Short-Day Bulb Onions
title_short Targeted Metabolome Profiling of Indonesian Shallots and Japanese Long-Day/Short-Day Bulb Onions
title_sort targeted metabolome profiling of indonesian shallots and japanese long-day/short-day bulb onions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9783499/
https://www.ncbi.nlm.nih.gov/pubmed/36557300
http://dx.doi.org/10.3390/metabo12121260
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