Cargando…

Investigation of the DNA Damage and Oxidative Effect Induced by Venlafaxine in Mouse Brain and Liver Cells

Venlafaxine is an antidepressant used worldwide. Therefore, studies to confirm its safe use are mandatory. This report evaluated the drug DNA damage capacity in the brain and liver of ICR mice, and its oxidative effect on DNA, lipids, and proteins, as well as the amount of nitrites, also in the brai...

Descripción completa

Detalles Bibliográficos
Autores principales: Madrigal-Bujaidar, Eduardo, Paniagua-Pérez, Rogelio, Rendón-Barrón, Michael Joshue, Morales-González, José Antonio, Madrigal-Santillán, Eduardo O., Álvarez-González, Isela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9783611/
https://www.ncbi.nlm.nih.gov/pubmed/36548570
http://dx.doi.org/10.3390/toxics10120737
_version_ 1784857618526765056
author Madrigal-Bujaidar, Eduardo
Paniagua-Pérez, Rogelio
Rendón-Barrón, Michael Joshue
Morales-González, José Antonio
Madrigal-Santillán, Eduardo O.
Álvarez-González, Isela
author_facet Madrigal-Bujaidar, Eduardo
Paniagua-Pérez, Rogelio
Rendón-Barrón, Michael Joshue
Morales-González, José Antonio
Madrigal-Santillán, Eduardo O.
Álvarez-González, Isela
author_sort Madrigal-Bujaidar, Eduardo
collection PubMed
description Venlafaxine is an antidepressant used worldwide. Therefore, studies to confirm its safe use are mandatory. This report evaluated the drug DNA damage capacity in the brain and liver of ICR mice, and its oxidative effect on DNA, lipids, and proteins, as well as the amount of nitrites, also in the brain and liver. Determinations were made at 2, 6, 12, and 24 h post-treatment, excluding DNA oxidation that was observed at 2 h. The tested doses of venlafaxine were 5, 50, and 250 mg/kg. The results showed DNA damage in the brain with the two more elevated doses of venlafaxine at 2 and 6 h post-treatment and also at 12 h in the liver. The comet assay plus the FPG enzyme showed DNA damage in both organs with all doses. The two high doses increased lipoperoxidation in the two tissues from 6 to 12 h post-administration. Protein oxidation increased with the three doses, mainly from 2 to 12 h, and nitrite content was elevated only with the high dose in the liver. The drug was found to affect both tissues, although it was more pronounced in the liver. Interestingly, DNA oxidative damage was observed even with a dose that corresponds to the therapeutic range. The clinical relevance of these findings awaits further investigations.
format Online
Article
Text
id pubmed-9783611
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-97836112022-12-24 Investigation of the DNA Damage and Oxidative Effect Induced by Venlafaxine in Mouse Brain and Liver Cells Madrigal-Bujaidar, Eduardo Paniagua-Pérez, Rogelio Rendón-Barrón, Michael Joshue Morales-González, José Antonio Madrigal-Santillán, Eduardo O. Álvarez-González, Isela Toxics Article Venlafaxine is an antidepressant used worldwide. Therefore, studies to confirm its safe use are mandatory. This report evaluated the drug DNA damage capacity in the brain and liver of ICR mice, and its oxidative effect on DNA, lipids, and proteins, as well as the amount of nitrites, also in the brain and liver. Determinations were made at 2, 6, 12, and 24 h post-treatment, excluding DNA oxidation that was observed at 2 h. The tested doses of venlafaxine were 5, 50, and 250 mg/kg. The results showed DNA damage in the brain with the two more elevated doses of venlafaxine at 2 and 6 h post-treatment and also at 12 h in the liver. The comet assay plus the FPG enzyme showed DNA damage in both organs with all doses. The two high doses increased lipoperoxidation in the two tissues from 6 to 12 h post-administration. Protein oxidation increased with the three doses, mainly from 2 to 12 h, and nitrite content was elevated only with the high dose in the liver. The drug was found to affect both tissues, although it was more pronounced in the liver. Interestingly, DNA oxidative damage was observed even with a dose that corresponds to the therapeutic range. The clinical relevance of these findings awaits further investigations. MDPI 2022-11-29 /pmc/articles/PMC9783611/ /pubmed/36548570 http://dx.doi.org/10.3390/toxics10120737 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Madrigal-Bujaidar, Eduardo
Paniagua-Pérez, Rogelio
Rendón-Barrón, Michael Joshue
Morales-González, José Antonio
Madrigal-Santillán, Eduardo O.
Álvarez-González, Isela
Investigation of the DNA Damage and Oxidative Effect Induced by Venlafaxine in Mouse Brain and Liver Cells
title Investigation of the DNA Damage and Oxidative Effect Induced by Venlafaxine in Mouse Brain and Liver Cells
title_full Investigation of the DNA Damage and Oxidative Effect Induced by Venlafaxine in Mouse Brain and Liver Cells
title_fullStr Investigation of the DNA Damage and Oxidative Effect Induced by Venlafaxine in Mouse Brain and Liver Cells
title_full_unstemmed Investigation of the DNA Damage and Oxidative Effect Induced by Venlafaxine in Mouse Brain and Liver Cells
title_short Investigation of the DNA Damage and Oxidative Effect Induced by Venlafaxine in Mouse Brain and Liver Cells
title_sort investigation of the dna damage and oxidative effect induced by venlafaxine in mouse brain and liver cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9783611/
https://www.ncbi.nlm.nih.gov/pubmed/36548570
http://dx.doi.org/10.3390/toxics10120737
work_keys_str_mv AT madrigalbujaidareduardo investigationofthednadamageandoxidativeeffectinducedbyvenlafaxineinmousebrainandlivercells
AT paniaguaperezrogelio investigationofthednadamageandoxidativeeffectinducedbyvenlafaxineinmousebrainandlivercells
AT rendonbarronmichaeljoshue investigationofthednadamageandoxidativeeffectinducedbyvenlafaxineinmousebrainandlivercells
AT moralesgonzalezjoseantonio investigationofthednadamageandoxidativeeffectinducedbyvenlafaxineinmousebrainandlivercells
AT madrigalsantillaneduardoo investigationofthednadamageandoxidativeeffectinducedbyvenlafaxineinmousebrainandlivercells
AT alvarezgonzalezisela investigationofthednadamageandoxidativeeffectinducedbyvenlafaxineinmousebrainandlivercells