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Long-Term Culture of Canine Ocular Cells That Maintain Canine Papillomaviruses
Canine ocular papillomas occur on the haired skin of eyelids, conjunctival epithelium, and rarely on the cornea. Using PCR typing assays with canine papillomavirus type-specific primer sets, our study confirmed that the papillomas contained canine papillomavirus type 1. The positive result from a ro...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9783655/ https://www.ncbi.nlm.nih.gov/pubmed/36560679 http://dx.doi.org/10.3390/v14122675 |
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author | Zhou, Dan Wang, Aibing Maxwell, Sarah Schlegel, Richard Yuan, Hang |
author_facet | Zhou, Dan Wang, Aibing Maxwell, Sarah Schlegel, Richard Yuan, Hang |
author_sort | Zhou, Dan |
collection | PubMed |
description | Canine ocular papillomas occur on the haired skin of eyelids, conjunctival epithelium, and rarely on the cornea. Using PCR typing assays with canine papillomavirus type-specific primer sets, our study confirmed that the papillomas contained canine papillomavirus type 1. The positive result from a rolling circle amplification assay indicated the CPV1 viral genome in the cells is a circular episomal form. We also successfully established the first canine corneal cell line using the conditional reprogramming method. The cells exhibited an epithelial cell morphology, grew rapidly in vitro, and could be maintained long term. For the continued growth of the canine corneal cells, feeder cells played a more important role than Rho-kinase inhibitor Y-27632. More importantly, the viral CPV1 genome was maintained in the canine corneal cells during the long-term expansion. Unlimited supplies of these cells provide as a model for the study CPV in dog cells, and a platform for drug screening for effective therapies against canine papillomavirus infection in the future. |
format | Online Article Text |
id | pubmed-9783655 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97836552022-12-24 Long-Term Culture of Canine Ocular Cells That Maintain Canine Papillomaviruses Zhou, Dan Wang, Aibing Maxwell, Sarah Schlegel, Richard Yuan, Hang Viruses Article Canine ocular papillomas occur on the haired skin of eyelids, conjunctival epithelium, and rarely on the cornea. Using PCR typing assays with canine papillomavirus type-specific primer sets, our study confirmed that the papillomas contained canine papillomavirus type 1. The positive result from a rolling circle amplification assay indicated the CPV1 viral genome in the cells is a circular episomal form. We also successfully established the first canine corneal cell line using the conditional reprogramming method. The cells exhibited an epithelial cell morphology, grew rapidly in vitro, and could be maintained long term. For the continued growth of the canine corneal cells, feeder cells played a more important role than Rho-kinase inhibitor Y-27632. More importantly, the viral CPV1 genome was maintained in the canine corneal cells during the long-term expansion. Unlimited supplies of these cells provide as a model for the study CPV in dog cells, and a platform for drug screening for effective therapies against canine papillomavirus infection in the future. MDPI 2022-11-29 /pmc/articles/PMC9783655/ /pubmed/36560679 http://dx.doi.org/10.3390/v14122675 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhou, Dan Wang, Aibing Maxwell, Sarah Schlegel, Richard Yuan, Hang Long-Term Culture of Canine Ocular Cells That Maintain Canine Papillomaviruses |
title | Long-Term Culture of Canine Ocular Cells That Maintain Canine Papillomaviruses |
title_full | Long-Term Culture of Canine Ocular Cells That Maintain Canine Papillomaviruses |
title_fullStr | Long-Term Culture of Canine Ocular Cells That Maintain Canine Papillomaviruses |
title_full_unstemmed | Long-Term Culture of Canine Ocular Cells That Maintain Canine Papillomaviruses |
title_short | Long-Term Culture of Canine Ocular Cells That Maintain Canine Papillomaviruses |
title_sort | long-term culture of canine ocular cells that maintain canine papillomaviruses |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9783655/ https://www.ncbi.nlm.nih.gov/pubmed/36560679 http://dx.doi.org/10.3390/v14122675 |
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