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Morphology of Lymphoid Tissue in the Lungs of Guinea Pigs Infected with Mycobacterium bovis against the Background of Vaccine Immunity and the Action of Betulin and Its Derivatives

Tuberculosis caused by Mycobacterium bovis is a serious problem for animal and human health worldwide. A promising concept for the design of anti-tuberculosis drugs is the conjugation of an immunogenic fraction isolated from bacterial vaccines with a stimulating component. Taking this principle as a...

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Autores principales: Koshkin, Ivan N., Vlasenko, Vasily S., Pleshakova, Valentina I., Alkhimova, Larisa E., Elyshev, Andrey V., Kulakov, Ivan V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9784198/
https://www.ncbi.nlm.nih.gov/pubmed/36560494
http://dx.doi.org/10.3390/vaccines10122084
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author Koshkin, Ivan N.
Vlasenko, Vasily S.
Pleshakova, Valentina I.
Alkhimova, Larisa E.
Elyshev, Andrey V.
Kulakov, Ivan V.
author_facet Koshkin, Ivan N.
Vlasenko, Vasily S.
Pleshakova, Valentina I.
Alkhimova, Larisa E.
Elyshev, Andrey V.
Kulakov, Ivan V.
author_sort Koshkin, Ivan N.
collection PubMed
description Tuberculosis caused by Mycobacterium bovis is a serious problem for animal and human health worldwide. A promising concept for the design of anti-tuberculosis drugs is the conjugation of an immunogenic fraction isolated from bacterial vaccines with a stimulating component. Taking this principle as a basis, conjugates based on BCG antigens with betulin and its derivatives (betulonic and betulinic acids) were designed. The aim of this research was to study the morphological changes in the lymphoid tissue associated with the bronchial mucosa lungs (BALT) in guinea pigs sensitized with experimental conjugates using a model of experimental tuberculosis. The results showed a significant decrease in the BALT response, expressed by a decrease in the diameter of lymphatic follicles and a decrease in their activity when exposed to conjugates based on BCG antigens with betulin and, especially, with betulonic acid, with a visually greater number of plasma cells observed in the lung tissues of guinea pigs of these groups. The absence of tuberculous foci and low BALT activity in the lungs of animals treated with betulin and betulonic acid are probably associated with the activation of humoral immunity under the action of these conjugates.
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spelling pubmed-97841982022-12-24 Morphology of Lymphoid Tissue in the Lungs of Guinea Pigs Infected with Mycobacterium bovis against the Background of Vaccine Immunity and the Action of Betulin and Its Derivatives Koshkin, Ivan N. Vlasenko, Vasily S. Pleshakova, Valentina I. Alkhimova, Larisa E. Elyshev, Andrey V. Kulakov, Ivan V. Vaccines (Basel) Article Tuberculosis caused by Mycobacterium bovis is a serious problem for animal and human health worldwide. A promising concept for the design of anti-tuberculosis drugs is the conjugation of an immunogenic fraction isolated from bacterial vaccines with a stimulating component. Taking this principle as a basis, conjugates based on BCG antigens with betulin and its derivatives (betulonic and betulinic acids) were designed. The aim of this research was to study the morphological changes in the lymphoid tissue associated with the bronchial mucosa lungs (BALT) in guinea pigs sensitized with experimental conjugates using a model of experimental tuberculosis. The results showed a significant decrease in the BALT response, expressed by a decrease in the diameter of lymphatic follicles and a decrease in their activity when exposed to conjugates based on BCG antigens with betulin and, especially, with betulonic acid, with a visually greater number of plasma cells observed in the lung tissues of guinea pigs of these groups. The absence of tuberculous foci and low BALT activity in the lungs of animals treated with betulin and betulonic acid are probably associated with the activation of humoral immunity under the action of these conjugates. MDPI 2022-12-06 /pmc/articles/PMC9784198/ /pubmed/36560494 http://dx.doi.org/10.3390/vaccines10122084 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Koshkin, Ivan N.
Vlasenko, Vasily S.
Pleshakova, Valentina I.
Alkhimova, Larisa E.
Elyshev, Andrey V.
Kulakov, Ivan V.
Morphology of Lymphoid Tissue in the Lungs of Guinea Pigs Infected with Mycobacterium bovis against the Background of Vaccine Immunity and the Action of Betulin and Its Derivatives
title Morphology of Lymphoid Tissue in the Lungs of Guinea Pigs Infected with Mycobacterium bovis against the Background of Vaccine Immunity and the Action of Betulin and Its Derivatives
title_full Morphology of Lymphoid Tissue in the Lungs of Guinea Pigs Infected with Mycobacterium bovis against the Background of Vaccine Immunity and the Action of Betulin and Its Derivatives
title_fullStr Morphology of Lymphoid Tissue in the Lungs of Guinea Pigs Infected with Mycobacterium bovis against the Background of Vaccine Immunity and the Action of Betulin and Its Derivatives
title_full_unstemmed Morphology of Lymphoid Tissue in the Lungs of Guinea Pigs Infected with Mycobacterium bovis against the Background of Vaccine Immunity and the Action of Betulin and Its Derivatives
title_short Morphology of Lymphoid Tissue in the Lungs of Guinea Pigs Infected with Mycobacterium bovis against the Background of Vaccine Immunity and the Action of Betulin and Its Derivatives
title_sort morphology of lymphoid tissue in the lungs of guinea pigs infected with mycobacterium bovis against the background of vaccine immunity and the action of betulin and its derivatives
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9784198/
https://www.ncbi.nlm.nih.gov/pubmed/36560494
http://dx.doi.org/10.3390/vaccines10122084
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