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Antiepileptic Effect of Neuroaid(®) on Strychnine-Induced Convulsions in Mice

NeuroAid II, a folk Chinese Medicine, is currently used in Asia for the treatment of stroke. An experimental study demonstrated that NeuroAid enables neuronal cells to be more resistant to glutamate toxicity. This research was constructed to evaluate the efficacy of NeuroAid in the prevention of epi...

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Autores principales: Salim Mahmood, Ahmed, Ammoo, Afaq M., Ali, Mayssam Hussein Mohammed, Hameed, Tiba M., Al-Hussaniy, Hany A., Aljumaili, Abdulla Amer Abbas, Al-Fallooji, Mohammed Hussein Alaa, Kadhim, Ali Hakim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9784395/
https://www.ncbi.nlm.nih.gov/pubmed/36558919
http://dx.doi.org/10.3390/ph15121468
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author Salim Mahmood, Ahmed
Ammoo, Afaq M.
Ali, Mayssam Hussein Mohammed
Hameed, Tiba M.
Al-Hussaniy, Hany A.
Aljumaili, Abdulla Amer Abbas
Al-Fallooji, Mohammed Hussein Alaa
Kadhim, Ali Hakim
author_facet Salim Mahmood, Ahmed
Ammoo, Afaq M.
Ali, Mayssam Hussein Mohammed
Hameed, Tiba M.
Al-Hussaniy, Hany A.
Aljumaili, Abdulla Amer Abbas
Al-Fallooji, Mohammed Hussein Alaa
Kadhim, Ali Hakim
author_sort Salim Mahmood, Ahmed
collection PubMed
description NeuroAid II, a folk Chinese Medicine, is currently used in Asia for the treatment of stroke. An experimental study demonstrated that NeuroAid enables neuronal cells to be more resistant to glutamate toxicity. This research was constructed to evaluate the efficacy of NeuroAid in the prevention of epilepsy (EP). Forty healthy adult male mice were used and divided into four groups (10 mice/group): normal control group; positive control group; NeuroAid-treated group (10 mg/kg); topiramate-treated group (10 mg/kg). The treatment continued for 7 days, and on the last day, EP was induced using strychnine at a dose of 2 mg/kg via intraperitoneal (ip) administration. Seizure severity, latency to the seizure onset, the number of seizures, and the duration of each seizure episode were observed for one hour. The death and protection rates over the next twenty-four hours were recorded. Brain specimens from surviving animals were extracted and examined pathologically for quantification of glutamate receptor (GluR) gene expression in the isolated hippocampus employing real-time PCR analysis. Treatment with NeuroAid resulted in a significant reduction in seizure severity, prolonged the onset of seizures, decreased the number and duration of episodes, reduced brain insult, and decreased mortality rate. Reductions in the gene expression of GluRs in the hippocampus with minor histopathological changes were observed in the NeruoAid- and topiramate-treated groups. It is concluded that NeuroAid has a potential antiepileptic effect (EP) with the ability to prevent convulsion through its effect on the glutamate receptor.
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spelling pubmed-97843952022-12-24 Antiepileptic Effect of Neuroaid(®) on Strychnine-Induced Convulsions in Mice Salim Mahmood, Ahmed Ammoo, Afaq M. Ali, Mayssam Hussein Mohammed Hameed, Tiba M. Al-Hussaniy, Hany A. Aljumaili, Abdulla Amer Abbas Al-Fallooji, Mohammed Hussein Alaa Kadhim, Ali Hakim Pharmaceuticals (Basel) Article NeuroAid II, a folk Chinese Medicine, is currently used in Asia for the treatment of stroke. An experimental study demonstrated that NeuroAid enables neuronal cells to be more resistant to glutamate toxicity. This research was constructed to evaluate the efficacy of NeuroAid in the prevention of epilepsy (EP). Forty healthy adult male mice were used and divided into four groups (10 mice/group): normal control group; positive control group; NeuroAid-treated group (10 mg/kg); topiramate-treated group (10 mg/kg). The treatment continued for 7 days, and on the last day, EP was induced using strychnine at a dose of 2 mg/kg via intraperitoneal (ip) administration. Seizure severity, latency to the seizure onset, the number of seizures, and the duration of each seizure episode were observed for one hour. The death and protection rates over the next twenty-four hours were recorded. Brain specimens from surviving animals were extracted and examined pathologically for quantification of glutamate receptor (GluR) gene expression in the isolated hippocampus employing real-time PCR analysis. Treatment with NeuroAid resulted in a significant reduction in seizure severity, prolonged the onset of seizures, decreased the number and duration of episodes, reduced brain insult, and decreased mortality rate. Reductions in the gene expression of GluRs in the hippocampus with minor histopathological changes were observed in the NeruoAid- and topiramate-treated groups. It is concluded that NeuroAid has a potential antiepileptic effect (EP) with the ability to prevent convulsion through its effect on the glutamate receptor. MDPI 2022-11-26 /pmc/articles/PMC9784395/ /pubmed/36558919 http://dx.doi.org/10.3390/ph15121468 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Salim Mahmood, Ahmed
Ammoo, Afaq M.
Ali, Mayssam Hussein Mohammed
Hameed, Tiba M.
Al-Hussaniy, Hany A.
Aljumaili, Abdulla Amer Abbas
Al-Fallooji, Mohammed Hussein Alaa
Kadhim, Ali Hakim
Antiepileptic Effect of Neuroaid(®) on Strychnine-Induced Convulsions in Mice
title Antiepileptic Effect of Neuroaid(®) on Strychnine-Induced Convulsions in Mice
title_full Antiepileptic Effect of Neuroaid(®) on Strychnine-Induced Convulsions in Mice
title_fullStr Antiepileptic Effect of Neuroaid(®) on Strychnine-Induced Convulsions in Mice
title_full_unstemmed Antiepileptic Effect of Neuroaid(®) on Strychnine-Induced Convulsions in Mice
title_short Antiepileptic Effect of Neuroaid(®) on Strychnine-Induced Convulsions in Mice
title_sort antiepileptic effect of neuroaid(®) on strychnine-induced convulsions in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9784395/
https://www.ncbi.nlm.nih.gov/pubmed/36558919
http://dx.doi.org/10.3390/ph15121468
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