Cargando…

Glycerol Monolaurate Alleviates Oxidative Stress and Intestinal Flora Imbalance Caused by Salinity Changes for Juvenile Grouper

Groupers with an initial body weight of 9.10 ± 0.03 g were selected to investigate whether dietary addition of 0 (G0) and 1800 mg/kg glycerol monolaurate (GML, G1800) could alleviate the oxidative stress response and intestinal flora imbalance after 0, 6, 12, and 24 h of salinity change in grouper....

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Xuehe, Zhu, Dongwenjun, Mao, Minling, Wu, Jianwei, Yang, Qihui, Tan, Beiping, Chi, Shuyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9784515/
https://www.ncbi.nlm.nih.gov/pubmed/36557306
http://dx.doi.org/10.3390/metabo12121268
_version_ 1784857830753304576
author Li, Xuehe
Zhu, Dongwenjun
Mao, Minling
Wu, Jianwei
Yang, Qihui
Tan, Beiping
Chi, Shuyan
author_facet Li, Xuehe
Zhu, Dongwenjun
Mao, Minling
Wu, Jianwei
Yang, Qihui
Tan, Beiping
Chi, Shuyan
author_sort Li, Xuehe
collection PubMed
description Groupers with an initial body weight of 9.10 ± 0.03 g were selected to investigate whether dietary addition of 0 (G0) and 1800 mg/kg glycerol monolaurate (GML, G1800) could alleviate the oxidative stress response and intestinal flora imbalance after 0, 6, 12, and 24 h of salinity change in grouper. Experimental results show that the dietary addition of GML significantly reduced the liver MDA content and increased the SOD activity of grouper. The gene expression of CAT and SOD increased and then decreased with time after adding 1800 mg/kg GML, and the highest values were significantly higher than those of the control group. Salinity change had a slight effect on the top four intestinal flora composition of grouper at 0, 12, and 24 h, with changes occurring only at 6 h when Cyanobacteria replaced Actinobacteria. The addition of dietary GML slowed down the intestinal flora disorder, inhibited the colonization of harmful bacterium Vibrio, and promoted the abundance of beneficial bacterium Bacillus. In conclusion, dietary GML significantly reduced the oxidative damage caused by sudden changes in salinity, improved the antioxidant capacity, and alleviated the intestinal flora imbalance in juvenile grouper.
format Online
Article
Text
id pubmed-9784515
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-97845152022-12-24 Glycerol Monolaurate Alleviates Oxidative Stress and Intestinal Flora Imbalance Caused by Salinity Changes for Juvenile Grouper Li, Xuehe Zhu, Dongwenjun Mao, Minling Wu, Jianwei Yang, Qihui Tan, Beiping Chi, Shuyan Metabolites Article Groupers with an initial body weight of 9.10 ± 0.03 g were selected to investigate whether dietary addition of 0 (G0) and 1800 mg/kg glycerol monolaurate (GML, G1800) could alleviate the oxidative stress response and intestinal flora imbalance after 0, 6, 12, and 24 h of salinity change in grouper. Experimental results show that the dietary addition of GML significantly reduced the liver MDA content and increased the SOD activity of grouper. The gene expression of CAT and SOD increased and then decreased with time after adding 1800 mg/kg GML, and the highest values were significantly higher than those of the control group. Salinity change had a slight effect on the top four intestinal flora composition of grouper at 0, 12, and 24 h, with changes occurring only at 6 h when Cyanobacteria replaced Actinobacteria. The addition of dietary GML slowed down the intestinal flora disorder, inhibited the colonization of harmful bacterium Vibrio, and promoted the abundance of beneficial bacterium Bacillus. In conclusion, dietary GML significantly reduced the oxidative damage caused by sudden changes in salinity, improved the antioxidant capacity, and alleviated the intestinal flora imbalance in juvenile grouper. MDPI 2022-12-15 /pmc/articles/PMC9784515/ /pubmed/36557306 http://dx.doi.org/10.3390/metabo12121268 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Xuehe
Zhu, Dongwenjun
Mao, Minling
Wu, Jianwei
Yang, Qihui
Tan, Beiping
Chi, Shuyan
Glycerol Monolaurate Alleviates Oxidative Stress and Intestinal Flora Imbalance Caused by Salinity Changes for Juvenile Grouper
title Glycerol Monolaurate Alleviates Oxidative Stress and Intestinal Flora Imbalance Caused by Salinity Changes for Juvenile Grouper
title_full Glycerol Monolaurate Alleviates Oxidative Stress and Intestinal Flora Imbalance Caused by Salinity Changes for Juvenile Grouper
title_fullStr Glycerol Monolaurate Alleviates Oxidative Stress and Intestinal Flora Imbalance Caused by Salinity Changes for Juvenile Grouper
title_full_unstemmed Glycerol Monolaurate Alleviates Oxidative Stress and Intestinal Flora Imbalance Caused by Salinity Changes for Juvenile Grouper
title_short Glycerol Monolaurate Alleviates Oxidative Stress and Intestinal Flora Imbalance Caused by Salinity Changes for Juvenile Grouper
title_sort glycerol monolaurate alleviates oxidative stress and intestinal flora imbalance caused by salinity changes for juvenile grouper
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9784515/
https://www.ncbi.nlm.nih.gov/pubmed/36557306
http://dx.doi.org/10.3390/metabo12121268
work_keys_str_mv AT lixuehe glycerolmonolauratealleviatesoxidativestressandintestinalfloraimbalancecausedbysalinitychangesforjuvenilegrouper
AT zhudongwenjun glycerolmonolauratealleviatesoxidativestressandintestinalfloraimbalancecausedbysalinitychangesforjuvenilegrouper
AT maominling glycerolmonolauratealleviatesoxidativestressandintestinalfloraimbalancecausedbysalinitychangesforjuvenilegrouper
AT wujianwei glycerolmonolauratealleviatesoxidativestressandintestinalfloraimbalancecausedbysalinitychangesforjuvenilegrouper
AT yangqihui glycerolmonolauratealleviatesoxidativestressandintestinalfloraimbalancecausedbysalinitychangesforjuvenilegrouper
AT tanbeiping glycerolmonolauratealleviatesoxidativestressandintestinalfloraimbalancecausedbysalinitychangesforjuvenilegrouper
AT chishuyan glycerolmonolauratealleviatesoxidativestressandintestinalfloraimbalancecausedbysalinitychangesforjuvenilegrouper