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Altered mRNA Expression of NFKB1 and NFKB2 Genes in Penile Lichen Sclerosus, Penile Cancer and Zoon Balanitis

Background. The nuclear factor–κB transcription factors 1 and 2 (NFKB1 and NFKB2) are key components of the NF-κB pathway, which responds to inflammatory signals. Since the NFKB1/2 factors are activated via different inflammatory molecules, we aimed to check their expression levels in penile cancer...

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Autores principales: Wierzbicki, Piotr M., Czajkowski, Mateusz, Kotulak-Chrząszcz, Anna, Bukowicz, Justyna, Dzieciuch, Klaudia, Sokołowska-Wojdyło, Małgorzata, Kmieć, Zbigniew, Matuszewski, Marcin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9784631/
https://www.ncbi.nlm.nih.gov/pubmed/36555871
http://dx.doi.org/10.3390/jcm11247254
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author Wierzbicki, Piotr M.
Czajkowski, Mateusz
Kotulak-Chrząszcz, Anna
Bukowicz, Justyna
Dzieciuch, Klaudia
Sokołowska-Wojdyło, Małgorzata
Kmieć, Zbigniew
Matuszewski, Marcin
author_facet Wierzbicki, Piotr M.
Czajkowski, Mateusz
Kotulak-Chrząszcz, Anna
Bukowicz, Justyna
Dzieciuch, Klaudia
Sokołowska-Wojdyło, Małgorzata
Kmieć, Zbigniew
Matuszewski, Marcin
author_sort Wierzbicki, Piotr M.
collection PubMed
description Background. The nuclear factor–κB transcription factors 1 and 2 (NFKB1 and NFKB2) are key components of the NF-κB pathway, which responds to inflammatory signals. Since the NFKB1/2 factors are activated via different inflammatory molecules, we aimed to check their expression levels in penile cancer (PC), penile dermatoses: lichen sclerosus (PLS) and zoon balanitis (ZB). Methods: Skin biopsies from altered and healthy looking foreskin were obtained from 59 (49 LS; early PLS: 13, moderate PLS: 32, severe PLS: 4; 6 PC; 4 ZB) and unchanged foreskin from 13 healthy control adult males undergoing circumcision. NFKB1/2 mRNA levels were quantified by qPCR. Results: The highest levels of NFKB1 and NFKB2 were observed in PC, ca. 22 and 3.5 times higher than in control, respectively. NFKB1 expression was correlated with PLS progression (rs = 0.667) and was ca. 20 times higher in advanced PLS than in controls and early PLS. Occurrence of micro-incontinence was associated with elevated NFKB1 levels in PLS. Conclusion: This is the first study regarding gene profiles of NFKB1/2 in PC and penile dermatoses. New drugs targeting modulation of canonical-activated NF–κB pathway should be studied and introduced to the treatment of PLS and PC apart from other treatments.
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spelling pubmed-97846312022-12-24 Altered mRNA Expression of NFKB1 and NFKB2 Genes in Penile Lichen Sclerosus, Penile Cancer and Zoon Balanitis Wierzbicki, Piotr M. Czajkowski, Mateusz Kotulak-Chrząszcz, Anna Bukowicz, Justyna Dzieciuch, Klaudia Sokołowska-Wojdyło, Małgorzata Kmieć, Zbigniew Matuszewski, Marcin J Clin Med Article Background. The nuclear factor–κB transcription factors 1 and 2 (NFKB1 and NFKB2) are key components of the NF-κB pathway, which responds to inflammatory signals. Since the NFKB1/2 factors are activated via different inflammatory molecules, we aimed to check their expression levels in penile cancer (PC), penile dermatoses: lichen sclerosus (PLS) and zoon balanitis (ZB). Methods: Skin biopsies from altered and healthy looking foreskin were obtained from 59 (49 LS; early PLS: 13, moderate PLS: 32, severe PLS: 4; 6 PC; 4 ZB) and unchanged foreskin from 13 healthy control adult males undergoing circumcision. NFKB1/2 mRNA levels were quantified by qPCR. Results: The highest levels of NFKB1 and NFKB2 were observed in PC, ca. 22 and 3.5 times higher than in control, respectively. NFKB1 expression was correlated with PLS progression (rs = 0.667) and was ca. 20 times higher in advanced PLS than in controls and early PLS. Occurrence of micro-incontinence was associated with elevated NFKB1 levels in PLS. Conclusion: This is the first study regarding gene profiles of NFKB1/2 in PC and penile dermatoses. New drugs targeting modulation of canonical-activated NF–κB pathway should be studied and introduced to the treatment of PLS and PC apart from other treatments. MDPI 2022-12-07 /pmc/articles/PMC9784631/ /pubmed/36555871 http://dx.doi.org/10.3390/jcm11247254 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wierzbicki, Piotr M.
Czajkowski, Mateusz
Kotulak-Chrząszcz, Anna
Bukowicz, Justyna
Dzieciuch, Klaudia
Sokołowska-Wojdyło, Małgorzata
Kmieć, Zbigniew
Matuszewski, Marcin
Altered mRNA Expression of NFKB1 and NFKB2 Genes in Penile Lichen Sclerosus, Penile Cancer and Zoon Balanitis
title Altered mRNA Expression of NFKB1 and NFKB2 Genes in Penile Lichen Sclerosus, Penile Cancer and Zoon Balanitis
title_full Altered mRNA Expression of NFKB1 and NFKB2 Genes in Penile Lichen Sclerosus, Penile Cancer and Zoon Balanitis
title_fullStr Altered mRNA Expression of NFKB1 and NFKB2 Genes in Penile Lichen Sclerosus, Penile Cancer and Zoon Balanitis
title_full_unstemmed Altered mRNA Expression of NFKB1 and NFKB2 Genes in Penile Lichen Sclerosus, Penile Cancer and Zoon Balanitis
title_short Altered mRNA Expression of NFKB1 and NFKB2 Genes in Penile Lichen Sclerosus, Penile Cancer and Zoon Balanitis
title_sort altered mrna expression of nfkb1 and nfkb2 genes in penile lichen sclerosus, penile cancer and zoon balanitis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9784631/
https://www.ncbi.nlm.nih.gov/pubmed/36555871
http://dx.doi.org/10.3390/jcm11247254
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