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Altered mRNA Expression of NFKB1 and NFKB2 Genes in Penile Lichen Sclerosus, Penile Cancer and Zoon Balanitis
Background. The nuclear factor–κB transcription factors 1 and 2 (NFKB1 and NFKB2) are key components of the NF-κB pathway, which responds to inflammatory signals. Since the NFKB1/2 factors are activated via different inflammatory molecules, we aimed to check their expression levels in penile cancer...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9784631/ https://www.ncbi.nlm.nih.gov/pubmed/36555871 http://dx.doi.org/10.3390/jcm11247254 |
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author | Wierzbicki, Piotr M. Czajkowski, Mateusz Kotulak-Chrząszcz, Anna Bukowicz, Justyna Dzieciuch, Klaudia Sokołowska-Wojdyło, Małgorzata Kmieć, Zbigniew Matuszewski, Marcin |
author_facet | Wierzbicki, Piotr M. Czajkowski, Mateusz Kotulak-Chrząszcz, Anna Bukowicz, Justyna Dzieciuch, Klaudia Sokołowska-Wojdyło, Małgorzata Kmieć, Zbigniew Matuszewski, Marcin |
author_sort | Wierzbicki, Piotr M. |
collection | PubMed |
description | Background. The nuclear factor–κB transcription factors 1 and 2 (NFKB1 and NFKB2) are key components of the NF-κB pathway, which responds to inflammatory signals. Since the NFKB1/2 factors are activated via different inflammatory molecules, we aimed to check their expression levels in penile cancer (PC), penile dermatoses: lichen sclerosus (PLS) and zoon balanitis (ZB). Methods: Skin biopsies from altered and healthy looking foreskin were obtained from 59 (49 LS; early PLS: 13, moderate PLS: 32, severe PLS: 4; 6 PC; 4 ZB) and unchanged foreskin from 13 healthy control adult males undergoing circumcision. NFKB1/2 mRNA levels were quantified by qPCR. Results: The highest levels of NFKB1 and NFKB2 were observed in PC, ca. 22 and 3.5 times higher than in control, respectively. NFKB1 expression was correlated with PLS progression (rs = 0.667) and was ca. 20 times higher in advanced PLS than in controls and early PLS. Occurrence of micro-incontinence was associated with elevated NFKB1 levels in PLS. Conclusion: This is the first study regarding gene profiles of NFKB1/2 in PC and penile dermatoses. New drugs targeting modulation of canonical-activated NF–κB pathway should be studied and introduced to the treatment of PLS and PC apart from other treatments. |
format | Online Article Text |
id | pubmed-9784631 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97846312022-12-24 Altered mRNA Expression of NFKB1 and NFKB2 Genes in Penile Lichen Sclerosus, Penile Cancer and Zoon Balanitis Wierzbicki, Piotr M. Czajkowski, Mateusz Kotulak-Chrząszcz, Anna Bukowicz, Justyna Dzieciuch, Klaudia Sokołowska-Wojdyło, Małgorzata Kmieć, Zbigniew Matuszewski, Marcin J Clin Med Article Background. The nuclear factor–κB transcription factors 1 and 2 (NFKB1 and NFKB2) are key components of the NF-κB pathway, which responds to inflammatory signals. Since the NFKB1/2 factors are activated via different inflammatory molecules, we aimed to check their expression levels in penile cancer (PC), penile dermatoses: lichen sclerosus (PLS) and zoon balanitis (ZB). Methods: Skin biopsies from altered and healthy looking foreskin were obtained from 59 (49 LS; early PLS: 13, moderate PLS: 32, severe PLS: 4; 6 PC; 4 ZB) and unchanged foreskin from 13 healthy control adult males undergoing circumcision. NFKB1/2 mRNA levels were quantified by qPCR. Results: The highest levels of NFKB1 and NFKB2 were observed in PC, ca. 22 and 3.5 times higher than in control, respectively. NFKB1 expression was correlated with PLS progression (rs = 0.667) and was ca. 20 times higher in advanced PLS than in controls and early PLS. Occurrence of micro-incontinence was associated with elevated NFKB1 levels in PLS. Conclusion: This is the first study regarding gene profiles of NFKB1/2 in PC and penile dermatoses. New drugs targeting modulation of canonical-activated NF–κB pathway should be studied and introduced to the treatment of PLS and PC apart from other treatments. MDPI 2022-12-07 /pmc/articles/PMC9784631/ /pubmed/36555871 http://dx.doi.org/10.3390/jcm11247254 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wierzbicki, Piotr M. Czajkowski, Mateusz Kotulak-Chrząszcz, Anna Bukowicz, Justyna Dzieciuch, Klaudia Sokołowska-Wojdyło, Małgorzata Kmieć, Zbigniew Matuszewski, Marcin Altered mRNA Expression of NFKB1 and NFKB2 Genes in Penile Lichen Sclerosus, Penile Cancer and Zoon Balanitis |
title | Altered mRNA Expression of NFKB1 and NFKB2 Genes in Penile Lichen Sclerosus, Penile Cancer and Zoon Balanitis |
title_full | Altered mRNA Expression of NFKB1 and NFKB2 Genes in Penile Lichen Sclerosus, Penile Cancer and Zoon Balanitis |
title_fullStr | Altered mRNA Expression of NFKB1 and NFKB2 Genes in Penile Lichen Sclerosus, Penile Cancer and Zoon Balanitis |
title_full_unstemmed | Altered mRNA Expression of NFKB1 and NFKB2 Genes in Penile Lichen Sclerosus, Penile Cancer and Zoon Balanitis |
title_short | Altered mRNA Expression of NFKB1 and NFKB2 Genes in Penile Lichen Sclerosus, Penile Cancer and Zoon Balanitis |
title_sort | altered mrna expression of nfkb1 and nfkb2 genes in penile lichen sclerosus, penile cancer and zoon balanitis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9784631/ https://www.ncbi.nlm.nih.gov/pubmed/36555871 http://dx.doi.org/10.3390/jcm11247254 |
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