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Effect of the Type VI Secretion System Secreted Protein Hcp on the Virulence of Aeromonas salmonicida

Aeromonas salmonicida, a psychrophilic bacterial pathogen, is widely distributed in marine freshwater, causing serious economic losses to major salmon farming areas in the world. At present, it is still one of the most important pathogens threatening salmon farming. Hcp (haemolysin-coregulated prote...

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Autores principales: Cai, Hongyan, Yu, Jiaying, Qiao, Ying, Ma, Ying, Zheng, Jiang, Lin, Mao, Yan, Qingpi, Huang, Lixing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9784854/
https://www.ncbi.nlm.nih.gov/pubmed/36557560
http://dx.doi.org/10.3390/microorganisms10122307
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author Cai, Hongyan
Yu, Jiaying
Qiao, Ying
Ma, Ying
Zheng, Jiang
Lin, Mao
Yan, Qingpi
Huang, Lixing
author_facet Cai, Hongyan
Yu, Jiaying
Qiao, Ying
Ma, Ying
Zheng, Jiang
Lin, Mao
Yan, Qingpi
Huang, Lixing
author_sort Cai, Hongyan
collection PubMed
description Aeromonas salmonicida, a psychrophilic bacterial pathogen, is widely distributed in marine freshwater, causing serious economic losses to major salmon farming areas in the world. At present, it is still one of the most important pathogens threatening salmon farming. Hcp (haemolysin-coregulated protein) is an effector protein in the type-VI secretion system (T6SS), which is secreted by T6SS and functions as its structural component. The results of our previous genomic sequencing showed that hcp existed in the mesophilic A. salmonicida SRW-OG1 isolated from naturally infected Epinephelus coioides. To further explore the role of Hcp in A. salmonicida SRW-OG1, we constructed an hcp-RNAi strain and verified its effect on the virulence of A. salmonicida. The results showed that compared with the wild strain, the hcp-RNAi strain suffered from different degrees of decreased adhesion, growth, biofilm formation, extracellular product secretion, and virulence. It was suggested that hcp may be an important virulence gene of A. salmonicida SRW-OG1.
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spelling pubmed-97848542022-12-24 Effect of the Type VI Secretion System Secreted Protein Hcp on the Virulence of Aeromonas salmonicida Cai, Hongyan Yu, Jiaying Qiao, Ying Ma, Ying Zheng, Jiang Lin, Mao Yan, Qingpi Huang, Lixing Microorganisms Article Aeromonas salmonicida, a psychrophilic bacterial pathogen, is widely distributed in marine freshwater, causing serious economic losses to major salmon farming areas in the world. At present, it is still one of the most important pathogens threatening salmon farming. Hcp (haemolysin-coregulated protein) is an effector protein in the type-VI secretion system (T6SS), which is secreted by T6SS and functions as its structural component. The results of our previous genomic sequencing showed that hcp existed in the mesophilic A. salmonicida SRW-OG1 isolated from naturally infected Epinephelus coioides. To further explore the role of Hcp in A. salmonicida SRW-OG1, we constructed an hcp-RNAi strain and verified its effect on the virulence of A. salmonicida. The results showed that compared with the wild strain, the hcp-RNAi strain suffered from different degrees of decreased adhesion, growth, biofilm formation, extracellular product secretion, and virulence. It was suggested that hcp may be an important virulence gene of A. salmonicida SRW-OG1. MDPI 2022-11-22 /pmc/articles/PMC9784854/ /pubmed/36557560 http://dx.doi.org/10.3390/microorganisms10122307 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cai, Hongyan
Yu, Jiaying
Qiao, Ying
Ma, Ying
Zheng, Jiang
Lin, Mao
Yan, Qingpi
Huang, Lixing
Effect of the Type VI Secretion System Secreted Protein Hcp on the Virulence of Aeromonas salmonicida
title Effect of the Type VI Secretion System Secreted Protein Hcp on the Virulence of Aeromonas salmonicida
title_full Effect of the Type VI Secretion System Secreted Protein Hcp on the Virulence of Aeromonas salmonicida
title_fullStr Effect of the Type VI Secretion System Secreted Protein Hcp on the Virulence of Aeromonas salmonicida
title_full_unstemmed Effect of the Type VI Secretion System Secreted Protein Hcp on the Virulence of Aeromonas salmonicida
title_short Effect of the Type VI Secretion System Secreted Protein Hcp on the Virulence of Aeromonas salmonicida
title_sort effect of the type vi secretion system secreted protein hcp on the virulence of aeromonas salmonicida
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9784854/
https://www.ncbi.nlm.nih.gov/pubmed/36557560
http://dx.doi.org/10.3390/microorganisms10122307
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