Cargando…

Dynamic Distribution of Infectious Pancreatic Necrosis Virus (IPNV) Strains of Genogroups 1, 5, and 7 after Intraperitoneal Administration in Rainbow Trout (Oncorhynchus mykiss)

Infectious pancreatic necrosis virus (IPNV) is the causative agent of rainbow trout (Oncorhynchus mykiss) IPN and causes significant loss of fingerlings. The currently prevalent IPNV genogroups in China are genogroups 1 and 5. However, in this study, we isolated and identified a novel IPNV, IPNV-P20...

Descripción completa

Detalles Bibliográficos
Autores principales: Shao, Yizhi, Ren, Guangming, Zhao, Jingzhuang, Lu, Tongyan, Liu, Qi, Xu, Liming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9784894/
https://www.ncbi.nlm.nih.gov/pubmed/36560638
http://dx.doi.org/10.3390/v14122634
_version_ 1784857920418086912
author Shao, Yizhi
Ren, Guangming
Zhao, Jingzhuang
Lu, Tongyan
Liu, Qi
Xu, Liming
author_facet Shao, Yizhi
Ren, Guangming
Zhao, Jingzhuang
Lu, Tongyan
Liu, Qi
Xu, Liming
author_sort Shao, Yizhi
collection PubMed
description Infectious pancreatic necrosis virus (IPNV) is the causative agent of rainbow trout (Oncorhynchus mykiss) IPN and causes significant loss of fingerlings. The currently prevalent IPNV genogroups in China are genogroups 1 and 5. However, in this study, we isolated and identified a novel IPNV, IPNV-P202019, which belonged to genogroup 7. Here, a total of 200 specific-pathogen-free rainbow trout (10 g average weight) were divided randomly into four groups to investigate the distribution of different IPNV strains (genogroups 1, 5, and 7) in 9 tissues of rainbow trout by means of intraperitoneal (ip) injection. Fish in each group were monitored after 3-, 7-, 14-, 21- and 28- days post-infection (dpi). The study showed no mortality in all groups. The distribution of IPNV genogroups 1 and 5 was similar in different tissues and had a higher number of viral loads after 3, 7, or 14 dpi. However, the distribution of IPNV genogroup 7 was detected particularly in the spleen, head kidney, and feces and had a lower number of viral loads. The results of this study provide valid data for the distribution of IPNV in rainbow trout tissues and showed that IPNV genogroups 1 and 5 were still the prevalent genogroups of IPNV in China. Although rainbow trout carried IPNV genogroup 7, the viral load was too low to be pathogenic.
format Online
Article
Text
id pubmed-9784894
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-97848942022-12-24 Dynamic Distribution of Infectious Pancreatic Necrosis Virus (IPNV) Strains of Genogroups 1, 5, and 7 after Intraperitoneal Administration in Rainbow Trout (Oncorhynchus mykiss) Shao, Yizhi Ren, Guangming Zhao, Jingzhuang Lu, Tongyan Liu, Qi Xu, Liming Viruses Article Infectious pancreatic necrosis virus (IPNV) is the causative agent of rainbow trout (Oncorhynchus mykiss) IPN and causes significant loss of fingerlings. The currently prevalent IPNV genogroups in China are genogroups 1 and 5. However, in this study, we isolated and identified a novel IPNV, IPNV-P202019, which belonged to genogroup 7. Here, a total of 200 specific-pathogen-free rainbow trout (10 g average weight) were divided randomly into four groups to investigate the distribution of different IPNV strains (genogroups 1, 5, and 7) in 9 tissues of rainbow trout by means of intraperitoneal (ip) injection. Fish in each group were monitored after 3-, 7-, 14-, 21- and 28- days post-infection (dpi). The study showed no mortality in all groups. The distribution of IPNV genogroups 1 and 5 was similar in different tissues and had a higher number of viral loads after 3, 7, or 14 dpi. However, the distribution of IPNV genogroup 7 was detected particularly in the spleen, head kidney, and feces and had a lower number of viral loads. The results of this study provide valid data for the distribution of IPNV in rainbow trout tissues and showed that IPNV genogroups 1 and 5 were still the prevalent genogroups of IPNV in China. Although rainbow trout carried IPNV genogroup 7, the viral load was too low to be pathogenic. MDPI 2022-11-25 /pmc/articles/PMC9784894/ /pubmed/36560638 http://dx.doi.org/10.3390/v14122634 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shao, Yizhi
Ren, Guangming
Zhao, Jingzhuang
Lu, Tongyan
Liu, Qi
Xu, Liming
Dynamic Distribution of Infectious Pancreatic Necrosis Virus (IPNV) Strains of Genogroups 1, 5, and 7 after Intraperitoneal Administration in Rainbow Trout (Oncorhynchus mykiss)
title Dynamic Distribution of Infectious Pancreatic Necrosis Virus (IPNV) Strains of Genogroups 1, 5, and 7 after Intraperitoneal Administration in Rainbow Trout (Oncorhynchus mykiss)
title_full Dynamic Distribution of Infectious Pancreatic Necrosis Virus (IPNV) Strains of Genogroups 1, 5, and 7 after Intraperitoneal Administration in Rainbow Trout (Oncorhynchus mykiss)
title_fullStr Dynamic Distribution of Infectious Pancreatic Necrosis Virus (IPNV) Strains of Genogroups 1, 5, and 7 after Intraperitoneal Administration in Rainbow Trout (Oncorhynchus mykiss)
title_full_unstemmed Dynamic Distribution of Infectious Pancreatic Necrosis Virus (IPNV) Strains of Genogroups 1, 5, and 7 after Intraperitoneal Administration in Rainbow Trout (Oncorhynchus mykiss)
title_short Dynamic Distribution of Infectious Pancreatic Necrosis Virus (IPNV) Strains of Genogroups 1, 5, and 7 after Intraperitoneal Administration in Rainbow Trout (Oncorhynchus mykiss)
title_sort dynamic distribution of infectious pancreatic necrosis virus (ipnv) strains of genogroups 1, 5, and 7 after intraperitoneal administration in rainbow trout (oncorhynchus mykiss)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9784894/
https://www.ncbi.nlm.nih.gov/pubmed/36560638
http://dx.doi.org/10.3390/v14122634
work_keys_str_mv AT shaoyizhi dynamicdistributionofinfectiouspancreaticnecrosisvirusipnvstrainsofgenogroups15and7afterintraperitonealadministrationinrainbowtroutoncorhynchusmykiss
AT renguangming dynamicdistributionofinfectiouspancreaticnecrosisvirusipnvstrainsofgenogroups15and7afterintraperitonealadministrationinrainbowtroutoncorhynchusmykiss
AT zhaojingzhuang dynamicdistributionofinfectiouspancreaticnecrosisvirusipnvstrainsofgenogroups15and7afterintraperitonealadministrationinrainbowtroutoncorhynchusmykiss
AT lutongyan dynamicdistributionofinfectiouspancreaticnecrosisvirusipnvstrainsofgenogroups15and7afterintraperitonealadministrationinrainbowtroutoncorhynchusmykiss
AT liuqi dynamicdistributionofinfectiouspancreaticnecrosisvirusipnvstrainsofgenogroups15and7afterintraperitonealadministrationinrainbowtroutoncorhynchusmykiss
AT xuliming dynamicdistributionofinfectiouspancreaticnecrosisvirusipnvstrainsofgenogroups15and7afterintraperitonealadministrationinrainbowtroutoncorhynchusmykiss