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Application of ORF3 Subunit Vaccine for Avian Hepatitis E Virus
SIMPLE SUMMARY: Avian hepatitis E has been widespread in many countries in the last few years and has caused immense economic losses to the poultry industry worldwide. There are still no commercial avian hepatitis E virus (HEV) vaccines so far. ORF3 protein subunit vaccines for HEV strain in laying...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9784926/ https://www.ncbi.nlm.nih.gov/pubmed/36548837 http://dx.doi.org/10.3390/vetsci9120676 |
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author | Yan, Hongjian Chi, Zengna Zhao, Hui Zhang, Yawen Zhang, Yuduo Wang, Yixin Chang, Shuang Zhao, Peng |
author_facet | Yan, Hongjian Chi, Zengna Zhao, Hui Zhang, Yawen Zhang, Yuduo Wang, Yixin Chang, Shuang Zhao, Peng |
author_sort | Yan, Hongjian |
collection | PubMed |
description | SIMPLE SUMMARY: Avian hepatitis E has been widespread in many countries in the last few years and has caused immense economic losses to the poultry industry worldwide. There are still no commercial avian hepatitis E virus (HEV) vaccines so far. ORF3 protein subunit vaccines for HEV strain in laying hens and broilers were prepared, respectively, in this study; and the challenge protection test confirmed that the vaccine could reduce the viral shedding in feces after HEV infection; it provides necessary technical reserve for the prevention and control of avian HEV under existing conditions. ABSTRACT: Avian hepatitis E virus (HEV) is the main etiologic pathogen of chicken big liver and spleen disease which is widely prevalent in China in recent years. However, due to the lack of a highly effective culture system in vitro, a genetically engineered subunit vaccine is the main direction of vaccine development. In this study, ORF3 genes of VaHEV strain from laying hens and YT-aHEV strain from broilers were amplified, respectively, and ORF3 protein was successfully expressed by Escherichia coli prokaryotic expression system. The serum samples were collected periodically to detect avian HEV antibodies by indirect immunofluorescence after specific pathogen free chickens immunized with the two proteins and their mixed proteins, the results showed that all serum samples were positive for antibodies to avian HEV. The antibody-positive chickens were artificially challenged with the cell-adapted strain YT-aHEV strain. The chickens from the immunized control group were infected successfully; no fecal detoxification was detected in the immunized group. In this study, two representative strains of ORF3 subunit vaccines of laying hens and broilers were prepared by prokaryotic expression, the immune effects of different proteins of these were evaluated through immunization and challenge studies in vivo, which provided a new technical possibility for prevention and control of avian HEV. |
format | Online Article Text |
id | pubmed-9784926 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97849262022-12-24 Application of ORF3 Subunit Vaccine for Avian Hepatitis E Virus Yan, Hongjian Chi, Zengna Zhao, Hui Zhang, Yawen Zhang, Yuduo Wang, Yixin Chang, Shuang Zhao, Peng Vet Sci Article SIMPLE SUMMARY: Avian hepatitis E has been widespread in many countries in the last few years and has caused immense economic losses to the poultry industry worldwide. There are still no commercial avian hepatitis E virus (HEV) vaccines so far. ORF3 protein subunit vaccines for HEV strain in laying hens and broilers were prepared, respectively, in this study; and the challenge protection test confirmed that the vaccine could reduce the viral shedding in feces after HEV infection; it provides necessary technical reserve for the prevention and control of avian HEV under existing conditions. ABSTRACT: Avian hepatitis E virus (HEV) is the main etiologic pathogen of chicken big liver and spleen disease which is widely prevalent in China in recent years. However, due to the lack of a highly effective culture system in vitro, a genetically engineered subunit vaccine is the main direction of vaccine development. In this study, ORF3 genes of VaHEV strain from laying hens and YT-aHEV strain from broilers were amplified, respectively, and ORF3 protein was successfully expressed by Escherichia coli prokaryotic expression system. The serum samples were collected periodically to detect avian HEV antibodies by indirect immunofluorescence after specific pathogen free chickens immunized with the two proteins and their mixed proteins, the results showed that all serum samples were positive for antibodies to avian HEV. The antibody-positive chickens were artificially challenged with the cell-adapted strain YT-aHEV strain. The chickens from the immunized control group were infected successfully; no fecal detoxification was detected in the immunized group. In this study, two representative strains of ORF3 subunit vaccines of laying hens and broilers were prepared by prokaryotic expression, the immune effects of different proteins of these were evaluated through immunization and challenge studies in vivo, which provided a new technical possibility for prevention and control of avian HEV. MDPI 2022-12-05 /pmc/articles/PMC9784926/ /pubmed/36548837 http://dx.doi.org/10.3390/vetsci9120676 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yan, Hongjian Chi, Zengna Zhao, Hui Zhang, Yawen Zhang, Yuduo Wang, Yixin Chang, Shuang Zhao, Peng Application of ORF3 Subunit Vaccine for Avian Hepatitis E Virus |
title | Application of ORF3 Subunit Vaccine for Avian Hepatitis E Virus |
title_full | Application of ORF3 Subunit Vaccine for Avian Hepatitis E Virus |
title_fullStr | Application of ORF3 Subunit Vaccine for Avian Hepatitis E Virus |
title_full_unstemmed | Application of ORF3 Subunit Vaccine for Avian Hepatitis E Virus |
title_short | Application of ORF3 Subunit Vaccine for Avian Hepatitis E Virus |
title_sort | application of orf3 subunit vaccine for avian hepatitis e virus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9784926/ https://www.ncbi.nlm.nih.gov/pubmed/36548837 http://dx.doi.org/10.3390/vetsci9120676 |
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