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Dysbiosis in Head and Neck Cancer: Determining Optimal Sampling Site for Oral Microbiome Collection

Recent research suggests that dysbiosis of the oral microbial community is associated with head and neck cancer (HNC). It remains unclear whether this dysbiosis causes chemo-radiotherapy (CRT)-related complications. However, to address this question, it is essential to determine the most representat...

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Autores principales: Pandey, Dheeraj, Szczesniak, Michal, Maclean, Julia, Yim, Howard Chi Ho, Zhang, Fan, Graham, Peter, El-Omar, Emad M., Wu, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9785010/
https://www.ncbi.nlm.nih.gov/pubmed/36558884
http://dx.doi.org/10.3390/pathogens11121550
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author Pandey, Dheeraj
Szczesniak, Michal
Maclean, Julia
Yim, Howard Chi Ho
Zhang, Fan
Graham, Peter
El-Omar, Emad M.
Wu, Peter
author_facet Pandey, Dheeraj
Szczesniak, Michal
Maclean, Julia
Yim, Howard Chi Ho
Zhang, Fan
Graham, Peter
El-Omar, Emad M.
Wu, Peter
author_sort Pandey, Dheeraj
collection PubMed
description Recent research suggests that dysbiosis of the oral microbial community is associated with head and neck cancer (HNC). It remains unclear whether this dysbiosis causes chemo-radiotherapy (CRT)-related complications. However, to address this question, it is essential to determine the most representative oral site for microbiome sampling. In this study, our purpose was to determine the optimal site for oral sample collection and whether the presence of HNC is associated with altered oral microbiome from this site. In 21 newly diagnosed HNC patients and 27 healthy controls, microbiome samples were collected from saliva, swabs from buccal mucosa, tongue, hard palate, faucial pillars and all mucosal sites combined. Microbial DNA was extracted and underwent 16S rRNA amplicon gene sequencing. In healthy controls, analysis of observed taxonomic units detected differences in alpha- and beta-diversity between sampling sites. Saliva was found to have the highest intra-community microbial diversity and lowest within-subject (temporal) and between-subject variance. Feature intersection showed that most species were shared between all sites, with saliva demonstrating the most unique species as well as highest overlap with other sites. In HNC patients, saliva was found to have the highest diversity but differences between sites were not statistically significant. Across all sites, HNC patients had lower alpha diversity than healthy controls. Beta-diversity analysis showed HNC patients’ microbiome to be compositionally distinct from healthy controls. This pattern was confirmed when the salivary microbiome was considered alone. HNC patients exhibited reduced diversity of the oral microbiome. Salivary samples demonstrate temporal stability, have the richest diversity and are sufficient to detect perturbation due to presence of HNC. Hence, they can be used as representative oral samples for microbiome studies in HNC patients.
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spelling pubmed-97850102022-12-24 Dysbiosis in Head and Neck Cancer: Determining Optimal Sampling Site for Oral Microbiome Collection Pandey, Dheeraj Szczesniak, Michal Maclean, Julia Yim, Howard Chi Ho Zhang, Fan Graham, Peter El-Omar, Emad M. Wu, Peter Pathogens Article Recent research suggests that dysbiosis of the oral microbial community is associated with head and neck cancer (HNC). It remains unclear whether this dysbiosis causes chemo-radiotherapy (CRT)-related complications. However, to address this question, it is essential to determine the most representative oral site for microbiome sampling. In this study, our purpose was to determine the optimal site for oral sample collection and whether the presence of HNC is associated with altered oral microbiome from this site. In 21 newly diagnosed HNC patients and 27 healthy controls, microbiome samples were collected from saliva, swabs from buccal mucosa, tongue, hard palate, faucial pillars and all mucosal sites combined. Microbial DNA was extracted and underwent 16S rRNA amplicon gene sequencing. In healthy controls, analysis of observed taxonomic units detected differences in alpha- and beta-diversity between sampling sites. Saliva was found to have the highest intra-community microbial diversity and lowest within-subject (temporal) and between-subject variance. Feature intersection showed that most species were shared between all sites, with saliva demonstrating the most unique species as well as highest overlap with other sites. In HNC patients, saliva was found to have the highest diversity but differences between sites were not statistically significant. Across all sites, HNC patients had lower alpha diversity than healthy controls. Beta-diversity analysis showed HNC patients’ microbiome to be compositionally distinct from healthy controls. This pattern was confirmed when the salivary microbiome was considered alone. HNC patients exhibited reduced diversity of the oral microbiome. Salivary samples demonstrate temporal stability, have the richest diversity and are sufficient to detect perturbation due to presence of HNC. Hence, they can be used as representative oral samples for microbiome studies in HNC patients. MDPI 2022-12-16 /pmc/articles/PMC9785010/ /pubmed/36558884 http://dx.doi.org/10.3390/pathogens11121550 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pandey, Dheeraj
Szczesniak, Michal
Maclean, Julia
Yim, Howard Chi Ho
Zhang, Fan
Graham, Peter
El-Omar, Emad M.
Wu, Peter
Dysbiosis in Head and Neck Cancer: Determining Optimal Sampling Site for Oral Microbiome Collection
title Dysbiosis in Head and Neck Cancer: Determining Optimal Sampling Site for Oral Microbiome Collection
title_full Dysbiosis in Head and Neck Cancer: Determining Optimal Sampling Site for Oral Microbiome Collection
title_fullStr Dysbiosis in Head and Neck Cancer: Determining Optimal Sampling Site for Oral Microbiome Collection
title_full_unstemmed Dysbiosis in Head and Neck Cancer: Determining Optimal Sampling Site for Oral Microbiome Collection
title_short Dysbiosis in Head and Neck Cancer: Determining Optimal Sampling Site for Oral Microbiome Collection
title_sort dysbiosis in head and neck cancer: determining optimal sampling site for oral microbiome collection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9785010/
https://www.ncbi.nlm.nih.gov/pubmed/36558884
http://dx.doi.org/10.3390/pathogens11121550
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