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CATASAN Is a New Anti-Biofilm Agent Produced by the Marine Antarctic Bacterium Psychrobacter sp. TAE2020

The development of new approaches to prevent microbial surface adhesion and biofilm formation is an emerging need following the growing understanding of the impact of biofilm-related infections on human health. Staphylococcus epidermidis, with its ability to form biofilm and colonize biomaterials, r...

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Autores principales: D’Angelo, Caterina, Casillo, Angela, Melchiorre, Chiara, Lauro, Concetta, Corsaro, Maria Michela, Carpentieri, Andrea, Tutino, Maria Luisa, Parrilli, Ermenegilda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9785100/
https://www.ncbi.nlm.nih.gov/pubmed/36547894
http://dx.doi.org/10.3390/md20120747
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author D’Angelo, Caterina
Casillo, Angela
Melchiorre, Chiara
Lauro, Concetta
Corsaro, Maria Michela
Carpentieri, Andrea
Tutino, Maria Luisa
Parrilli, Ermenegilda
author_facet D’Angelo, Caterina
Casillo, Angela
Melchiorre, Chiara
Lauro, Concetta
Corsaro, Maria Michela
Carpentieri, Andrea
Tutino, Maria Luisa
Parrilli, Ermenegilda
author_sort D’Angelo, Caterina
collection PubMed
description The development of new approaches to prevent microbial surface adhesion and biofilm formation is an emerging need following the growing understanding of the impact of biofilm-related infections on human health. Staphylococcus epidermidis, with its ability to form biofilm and colonize biomaterials, represents the most frequent causative agent involved in infections of medical devices. In the research of new anti-biofilm agents against S. epidermidis biofilm, Antarctic marine bacteria represent an untapped reservoir of biodiversity. In the present study, the attention was focused on Psychrobacter sp. TAE2020, an Antarctic marine bacterium that produces molecules able to impair the initial attachment of S. epidermidis strains to the polystyrene surface. The setup of suitable purification protocols allowed the identification by NMR spectroscopy and LC-MS/MS analysis of a protein–polysaccharide complex named CATASAN. This complex proved to be a very effective anti-biofilm agent. Indeed, it not only interferes with cell surface attachment, but also prevents biofilm formation and affects the mature biofilm matrix structure of S. epidermidis. Moreover, CATASAN is endowed with a good emulsification activity in a wide range of pH and temperature. Therefore, its use can be easily extended to different biotechnological applications.
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spelling pubmed-97851002022-12-24 CATASAN Is a New Anti-Biofilm Agent Produced by the Marine Antarctic Bacterium Psychrobacter sp. TAE2020 D’Angelo, Caterina Casillo, Angela Melchiorre, Chiara Lauro, Concetta Corsaro, Maria Michela Carpentieri, Andrea Tutino, Maria Luisa Parrilli, Ermenegilda Mar Drugs Article The development of new approaches to prevent microbial surface adhesion and biofilm formation is an emerging need following the growing understanding of the impact of biofilm-related infections on human health. Staphylococcus epidermidis, with its ability to form biofilm and colonize biomaterials, represents the most frequent causative agent involved in infections of medical devices. In the research of new anti-biofilm agents against S. epidermidis biofilm, Antarctic marine bacteria represent an untapped reservoir of biodiversity. In the present study, the attention was focused on Psychrobacter sp. TAE2020, an Antarctic marine bacterium that produces molecules able to impair the initial attachment of S. epidermidis strains to the polystyrene surface. The setup of suitable purification protocols allowed the identification by NMR spectroscopy and LC-MS/MS analysis of a protein–polysaccharide complex named CATASAN. This complex proved to be a very effective anti-biofilm agent. Indeed, it not only interferes with cell surface attachment, but also prevents biofilm formation and affects the mature biofilm matrix structure of S. epidermidis. Moreover, CATASAN is endowed with a good emulsification activity in a wide range of pH and temperature. Therefore, its use can be easily extended to different biotechnological applications. MDPI 2022-11-27 /pmc/articles/PMC9785100/ /pubmed/36547894 http://dx.doi.org/10.3390/md20120747 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
D’Angelo, Caterina
Casillo, Angela
Melchiorre, Chiara
Lauro, Concetta
Corsaro, Maria Michela
Carpentieri, Andrea
Tutino, Maria Luisa
Parrilli, Ermenegilda
CATASAN Is a New Anti-Biofilm Agent Produced by the Marine Antarctic Bacterium Psychrobacter sp. TAE2020
title CATASAN Is a New Anti-Biofilm Agent Produced by the Marine Antarctic Bacterium Psychrobacter sp. TAE2020
title_full CATASAN Is a New Anti-Biofilm Agent Produced by the Marine Antarctic Bacterium Psychrobacter sp. TAE2020
title_fullStr CATASAN Is a New Anti-Biofilm Agent Produced by the Marine Antarctic Bacterium Psychrobacter sp. TAE2020
title_full_unstemmed CATASAN Is a New Anti-Biofilm Agent Produced by the Marine Antarctic Bacterium Psychrobacter sp. TAE2020
title_short CATASAN Is a New Anti-Biofilm Agent Produced by the Marine Antarctic Bacterium Psychrobacter sp. TAE2020
title_sort catasan is a new anti-biofilm agent produced by the marine antarctic bacterium psychrobacter sp. tae2020
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9785100/
https://www.ncbi.nlm.nih.gov/pubmed/36547894
http://dx.doi.org/10.3390/md20120747
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