Development of a Proton-Enhanced ESI UPLC-MS/MS Method for the Determination of Tetrodotoxin

Tetrodotoxin (TTX) is a kind of low-molecular-weight non-protein neurotoxin. It is one of the most potent neurotoxins found in nature, and it is found in puffer fish and various marine biota. The low sensitivity of previous analytical methods limited their application in puffer fish organ samples. T...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Tong, Wang, Ruiguo, Wang, Peilong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9785107/
https://www.ncbi.nlm.nih.gov/pubmed/36558099
http://dx.doi.org/10.3390/molecules27248967
_version_ 1784857968287678464
author Li, Tong
Wang, Ruiguo
Wang, Peilong
author_facet Li, Tong
Wang, Ruiguo
Wang, Peilong
author_sort Li, Tong
collection PubMed
description Tetrodotoxin (TTX) is a kind of low-molecular-weight non-protein neurotoxin. It is one of the most potent neurotoxins found in nature, and it is found in puffer fish and various marine biota. The low sensitivity of previous analytical methods limited their application in puffer fish organ samples. This study established a method for the accurate and fast determination of TTX by reversed ultra-performance liquid chromatography coupled with proton-enhanced electron spray ionization–tandem mass spectrometry. The method yields good peak shapes, high sensitivity and low coeluted interferences. The method was successfully applied to determine TTX in puffer fish tissue samples of about 0.2 g.
format Online
Article
Text
id pubmed-9785107
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-97851072022-12-24 Development of a Proton-Enhanced ESI UPLC-MS/MS Method for the Determination of Tetrodotoxin Li, Tong Wang, Ruiguo Wang, Peilong Molecules Article Tetrodotoxin (TTX) is a kind of low-molecular-weight non-protein neurotoxin. It is one of the most potent neurotoxins found in nature, and it is found in puffer fish and various marine biota. The low sensitivity of previous analytical methods limited their application in puffer fish organ samples. This study established a method for the accurate and fast determination of TTX by reversed ultra-performance liquid chromatography coupled with proton-enhanced electron spray ionization–tandem mass spectrometry. The method yields good peak shapes, high sensitivity and low coeluted interferences. The method was successfully applied to determine TTX in puffer fish tissue samples of about 0.2 g. MDPI 2022-12-16 /pmc/articles/PMC9785107/ /pubmed/36558099 http://dx.doi.org/10.3390/molecules27248967 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Tong
Wang, Ruiguo
Wang, Peilong
Development of a Proton-Enhanced ESI UPLC-MS/MS Method for the Determination of Tetrodotoxin
title Development of a Proton-Enhanced ESI UPLC-MS/MS Method for the Determination of Tetrodotoxin
title_full Development of a Proton-Enhanced ESI UPLC-MS/MS Method for the Determination of Tetrodotoxin
title_fullStr Development of a Proton-Enhanced ESI UPLC-MS/MS Method for the Determination of Tetrodotoxin
title_full_unstemmed Development of a Proton-Enhanced ESI UPLC-MS/MS Method for the Determination of Tetrodotoxin
title_short Development of a Proton-Enhanced ESI UPLC-MS/MS Method for the Determination of Tetrodotoxin
title_sort development of a proton-enhanced esi uplc-ms/ms method for the determination of tetrodotoxin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9785107/
https://www.ncbi.nlm.nih.gov/pubmed/36558099
http://dx.doi.org/10.3390/molecules27248967
work_keys_str_mv AT litong developmentofaprotonenhancedesiuplcmsmsmethodforthedeterminationoftetrodotoxin
AT wangruiguo developmentofaprotonenhancedesiuplcmsmsmethodforthedeterminationoftetrodotoxin
AT wangpeilong developmentofaprotonenhancedesiuplcmsmsmethodforthedeterminationoftetrodotoxin

Ejemplares similares