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Metformin Acutely Mitigates Oxidative Stress in Human Atrial Tissue: A Pilot Study in Overweight Non-Diabetic Cardiac Patients

Metformin, the first-line drug in type 2 diabetes mellitus, elicits cardiovascular protection also in obese patients via pleiotropic effects, among which the anti-oxidant is one of the most investigated. The aim of the present study was to assess whether metformin can acutely mitigate oxidative stre...

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Autores principales: Lascu, Ana, Ionică, Loredana-Nicoleta, Merce, Adrian-Petru, Dănilă, Maria-Daniela, Petrescu, Lucian, Sturza, Adrian, Muntean, Danina-Mirela, Streian, Caius Glad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9785172/
https://www.ncbi.nlm.nih.gov/pubmed/36556423
http://dx.doi.org/10.3390/life12122058
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author Lascu, Ana
Ionică, Loredana-Nicoleta
Merce, Adrian-Petru
Dănilă, Maria-Daniela
Petrescu, Lucian
Sturza, Adrian
Muntean, Danina-Mirela
Streian, Caius Glad
author_facet Lascu, Ana
Ionică, Loredana-Nicoleta
Merce, Adrian-Petru
Dănilă, Maria-Daniela
Petrescu, Lucian
Sturza, Adrian
Muntean, Danina-Mirela
Streian, Caius Glad
author_sort Lascu, Ana
collection PubMed
description Metformin, the first-line drug in type 2 diabetes mellitus, elicits cardiovascular protection also in obese patients via pleiotropic effects, among which the anti-oxidant is one of the most investigated. The aim of the present study was to assess whether metformin can acutely mitigate oxidative stress in atrial tissue harvested from overweight non-diabetic patients. Right atrial appendage samples were harvested during open-heart surgery and used for the evaluation of reactive oxygen species (ROS) production by means of confocal microscopy (superoxide anion) and spectrophotometry (hydrogen peroxide). Experiments were performed after acute incubation with metformin (10 µM) in the presence vs. absence of angiotensin II (AII, 100 nM), lipopolysaccharide (LPS, 1 μg/mL), and high glucose (Gluc, 400 mg/dL). Stimulation with AII, LPS, and high Gluc increased ROS production. The magnitude of oxidative stress correlated with several echocardiographic parameters. Metformin applied in the lowest therapeutic concentration (10 µM) was able to decrease ROS generation in stimulated but also non-stimulated atrial samples. In conclusion, in a pilot group of overweight non-diabetic cardiac patients, acute incubation with metformin at a clinically relevant dose alleviated oxidative stress both in basal conditions and conditions that mimicked the activation of the renin–angiotensin–aldosterone system, acute inflammation, and uncontrolled hyperglycemia.
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spelling pubmed-97851722022-12-24 Metformin Acutely Mitigates Oxidative Stress in Human Atrial Tissue: A Pilot Study in Overweight Non-Diabetic Cardiac Patients Lascu, Ana Ionică, Loredana-Nicoleta Merce, Adrian-Petru Dănilă, Maria-Daniela Petrescu, Lucian Sturza, Adrian Muntean, Danina-Mirela Streian, Caius Glad Life (Basel) Article Metformin, the first-line drug in type 2 diabetes mellitus, elicits cardiovascular protection also in obese patients via pleiotropic effects, among which the anti-oxidant is one of the most investigated. The aim of the present study was to assess whether metformin can acutely mitigate oxidative stress in atrial tissue harvested from overweight non-diabetic patients. Right atrial appendage samples were harvested during open-heart surgery and used for the evaluation of reactive oxygen species (ROS) production by means of confocal microscopy (superoxide anion) and spectrophotometry (hydrogen peroxide). Experiments were performed after acute incubation with metformin (10 µM) in the presence vs. absence of angiotensin II (AII, 100 nM), lipopolysaccharide (LPS, 1 μg/mL), and high glucose (Gluc, 400 mg/dL). Stimulation with AII, LPS, and high Gluc increased ROS production. The magnitude of oxidative stress correlated with several echocardiographic parameters. Metformin applied in the lowest therapeutic concentration (10 µM) was able to decrease ROS generation in stimulated but also non-stimulated atrial samples. In conclusion, in a pilot group of overweight non-diabetic cardiac patients, acute incubation with metformin at a clinically relevant dose alleviated oxidative stress both in basal conditions and conditions that mimicked the activation of the renin–angiotensin–aldosterone system, acute inflammation, and uncontrolled hyperglycemia. MDPI 2022-12-08 /pmc/articles/PMC9785172/ /pubmed/36556423 http://dx.doi.org/10.3390/life12122058 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lascu, Ana
Ionică, Loredana-Nicoleta
Merce, Adrian-Petru
Dănilă, Maria-Daniela
Petrescu, Lucian
Sturza, Adrian
Muntean, Danina-Mirela
Streian, Caius Glad
Metformin Acutely Mitigates Oxidative Stress in Human Atrial Tissue: A Pilot Study in Overweight Non-Diabetic Cardiac Patients
title Metformin Acutely Mitigates Oxidative Stress in Human Atrial Tissue: A Pilot Study in Overweight Non-Diabetic Cardiac Patients
title_full Metformin Acutely Mitigates Oxidative Stress in Human Atrial Tissue: A Pilot Study in Overweight Non-Diabetic Cardiac Patients
title_fullStr Metformin Acutely Mitigates Oxidative Stress in Human Atrial Tissue: A Pilot Study in Overweight Non-Diabetic Cardiac Patients
title_full_unstemmed Metformin Acutely Mitigates Oxidative Stress in Human Atrial Tissue: A Pilot Study in Overweight Non-Diabetic Cardiac Patients
title_short Metformin Acutely Mitigates Oxidative Stress in Human Atrial Tissue: A Pilot Study in Overweight Non-Diabetic Cardiac Patients
title_sort metformin acutely mitigates oxidative stress in human atrial tissue: a pilot study in overweight non-diabetic cardiac patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9785172/
https://www.ncbi.nlm.nih.gov/pubmed/36556423
http://dx.doi.org/10.3390/life12122058
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