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Metagenome-Based Exploration of Bacterial Communities Associated with Cyanobacteria Strains Isolated from Thermal Muds

Cyanobacteria constitute a pioneer colonizer of specific environments for whom settlement in new biotopes precedes the establishment of composite microbial consortia. Some heterotrophic bacteria constitute cyanobacterial partners that are considered as their cyanosphere, being potentially involved i...

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Autores principales: Halary, Sébastien, Duperron, Sébastien, Demay, Justine, Duval, Charlotte, Hamlaoui, Sahima, Piquet, Bérénice, Reinhardt, Anita, Bernard, Cécile, Marie, Benjamin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9785279/
https://www.ncbi.nlm.nih.gov/pubmed/36557590
http://dx.doi.org/10.3390/microorganisms10122337
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author Halary, Sébastien
Duperron, Sébastien
Demay, Justine
Duval, Charlotte
Hamlaoui, Sahima
Piquet, Bérénice
Reinhardt, Anita
Bernard, Cécile
Marie, Benjamin
author_facet Halary, Sébastien
Duperron, Sébastien
Demay, Justine
Duval, Charlotte
Hamlaoui, Sahima
Piquet, Bérénice
Reinhardt, Anita
Bernard, Cécile
Marie, Benjamin
author_sort Halary, Sébastien
collection PubMed
description Cyanobacteria constitute a pioneer colonizer of specific environments for whom settlement in new biotopes precedes the establishment of composite microbial consortia. Some heterotrophic bacteria constitute cyanobacterial partners that are considered as their cyanosphere, being potentially involved in mutualistic relationships through the exchange and recycling of key nutrients and the sharing of common goods. Several non-axenic cyanobacterial strains have been recently isolated, along with their associated cyanospheres, from the thermal mud of Balaruc-les-Bains (France) and the biofilms of the retention basin where they develop. The community structure and relationships among the members of the isolated cyanobacterial strains were characterized using a metagenomic approach combined with taxonomic and microscopic descriptions of the microbial consortia. The results provided insights into the potential role and metabolic capabilities of the microorganisms of thermal mud-associated cyanobacterial biofilms. Thus, the physical proximity, host-specificity, and genetic potential functions advocate for their complementarity between cyanobacteria and their associated microbiota. Besides these findings, our results also highlighted the great influence of the reference protein database chosen for performing functional annotation of the metagenomes from organisms of the cyanosphere and the difficulty of selecting one unique database that appropriately covers both autotroph and heterotroph metabolic specificities.
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spelling pubmed-97852792022-12-24 Metagenome-Based Exploration of Bacterial Communities Associated with Cyanobacteria Strains Isolated from Thermal Muds Halary, Sébastien Duperron, Sébastien Demay, Justine Duval, Charlotte Hamlaoui, Sahima Piquet, Bérénice Reinhardt, Anita Bernard, Cécile Marie, Benjamin Microorganisms Article Cyanobacteria constitute a pioneer colonizer of specific environments for whom settlement in new biotopes precedes the establishment of composite microbial consortia. Some heterotrophic bacteria constitute cyanobacterial partners that are considered as their cyanosphere, being potentially involved in mutualistic relationships through the exchange and recycling of key nutrients and the sharing of common goods. Several non-axenic cyanobacterial strains have been recently isolated, along with their associated cyanospheres, from the thermal mud of Balaruc-les-Bains (France) and the biofilms of the retention basin where they develop. The community structure and relationships among the members of the isolated cyanobacterial strains were characterized using a metagenomic approach combined with taxonomic and microscopic descriptions of the microbial consortia. The results provided insights into the potential role and metabolic capabilities of the microorganisms of thermal mud-associated cyanobacterial biofilms. Thus, the physical proximity, host-specificity, and genetic potential functions advocate for their complementarity between cyanobacteria and their associated microbiota. Besides these findings, our results also highlighted the great influence of the reference protein database chosen for performing functional annotation of the metagenomes from organisms of the cyanosphere and the difficulty of selecting one unique database that appropriately covers both autotroph and heterotroph metabolic specificities. MDPI 2022-11-25 /pmc/articles/PMC9785279/ /pubmed/36557590 http://dx.doi.org/10.3390/microorganisms10122337 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Halary, Sébastien
Duperron, Sébastien
Demay, Justine
Duval, Charlotte
Hamlaoui, Sahima
Piquet, Bérénice
Reinhardt, Anita
Bernard, Cécile
Marie, Benjamin
Metagenome-Based Exploration of Bacterial Communities Associated with Cyanobacteria Strains Isolated from Thermal Muds
title Metagenome-Based Exploration of Bacterial Communities Associated with Cyanobacteria Strains Isolated from Thermal Muds
title_full Metagenome-Based Exploration of Bacterial Communities Associated with Cyanobacteria Strains Isolated from Thermal Muds
title_fullStr Metagenome-Based Exploration of Bacterial Communities Associated with Cyanobacteria Strains Isolated from Thermal Muds
title_full_unstemmed Metagenome-Based Exploration of Bacterial Communities Associated with Cyanobacteria Strains Isolated from Thermal Muds
title_short Metagenome-Based Exploration of Bacterial Communities Associated with Cyanobacteria Strains Isolated from Thermal Muds
title_sort metagenome-based exploration of bacterial communities associated with cyanobacteria strains isolated from thermal muds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9785279/
https://www.ncbi.nlm.nih.gov/pubmed/36557590
http://dx.doi.org/10.3390/microorganisms10122337
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