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Interaction of Colloidal Gold Nanoparticles with Urine and Saliva Biofluids: An Exploratory Study

The use of gold nanoparticles for drug delivery, photothermal or photodynamic therapy, and biosensing enhances the demand for knowledge about the protein corona formed on the surface of nanoparticles. In this study, gold nanospheres (AuNSs), gold nanorods (AuNRs), and gold nanoflowers (AuNFs) were i...

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Autores principales: António, Maria, Lima, Tânia, Vitorino, Rui, Daniel-da-Silva, Ana L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9785464/
https://www.ncbi.nlm.nih.gov/pubmed/36558287
http://dx.doi.org/10.3390/nano12244434
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author António, Maria
Lima, Tânia
Vitorino, Rui
Daniel-da-Silva, Ana L.
author_facet António, Maria
Lima, Tânia
Vitorino, Rui
Daniel-da-Silva, Ana L.
author_sort António, Maria
collection PubMed
description The use of gold nanoparticles for drug delivery, photothermal or photodynamic therapy, and biosensing enhances the demand for knowledge about the protein corona formed on the surface of nanoparticles. In this study, gold nanospheres (AuNSs), gold nanorods (AuNRs), and gold nanoflowers (AuNFs) were incubated with saliva or urine. After the interaction, the surface of gold nanoparticles was investigated using UV-VIS spectroscopy, zeta potential, and dynamic light scattering. The shifting of the localized surface plasmon resonance (LSPR) band, the increase in hydrodynamic diameter, and the changes in the surface charge of nanoparticles indicated the presence of biomolecules on the surface of AuNSs, AuNRs, and AuNFs. The incubation of AuNFs with saliva led to nanoparticle aggregation and minimal protein adsorption. AuNSs and AuNRs incubated in saliva were analyzed through liquid chromatography with tandem mass spectrometry (LC-MS/MS) to identify the 96 proteins adsorbed on the surface of the gold nanoparticles. Among the 20 most abundant proteins identified, 14 proteins were common in both AuNSs and AuNRs. We hypothesize that the adsorption of these proteins was due to their high sulfur content, allowing for their interaction with gold nanoparticles via the Au-S bond. The presence of distinct proteins on the surface of AuNSs or AuNRs was also investigated and possibly related to the competition between proteins present on the external layers of corona and gold nanoparticle morphology.
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spelling pubmed-97854642022-12-24 Interaction of Colloidal Gold Nanoparticles with Urine and Saliva Biofluids: An Exploratory Study António, Maria Lima, Tânia Vitorino, Rui Daniel-da-Silva, Ana L. Nanomaterials (Basel) Article The use of gold nanoparticles for drug delivery, photothermal or photodynamic therapy, and biosensing enhances the demand for knowledge about the protein corona formed on the surface of nanoparticles. In this study, gold nanospheres (AuNSs), gold nanorods (AuNRs), and gold nanoflowers (AuNFs) were incubated with saliva or urine. After the interaction, the surface of gold nanoparticles was investigated using UV-VIS spectroscopy, zeta potential, and dynamic light scattering. The shifting of the localized surface plasmon resonance (LSPR) band, the increase in hydrodynamic diameter, and the changes in the surface charge of nanoparticles indicated the presence of biomolecules on the surface of AuNSs, AuNRs, and AuNFs. The incubation of AuNFs with saliva led to nanoparticle aggregation and minimal protein adsorption. AuNSs and AuNRs incubated in saliva were analyzed through liquid chromatography with tandem mass spectrometry (LC-MS/MS) to identify the 96 proteins adsorbed on the surface of the gold nanoparticles. Among the 20 most abundant proteins identified, 14 proteins were common in both AuNSs and AuNRs. We hypothesize that the adsorption of these proteins was due to their high sulfur content, allowing for their interaction with gold nanoparticles via the Au-S bond. The presence of distinct proteins on the surface of AuNSs or AuNRs was also investigated and possibly related to the competition between proteins present on the external layers of corona and gold nanoparticle morphology. MDPI 2022-12-13 /pmc/articles/PMC9785464/ /pubmed/36558287 http://dx.doi.org/10.3390/nano12244434 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
António, Maria
Lima, Tânia
Vitorino, Rui
Daniel-da-Silva, Ana L.
Interaction of Colloidal Gold Nanoparticles with Urine and Saliva Biofluids: An Exploratory Study
title Interaction of Colloidal Gold Nanoparticles with Urine and Saliva Biofluids: An Exploratory Study
title_full Interaction of Colloidal Gold Nanoparticles with Urine and Saliva Biofluids: An Exploratory Study
title_fullStr Interaction of Colloidal Gold Nanoparticles with Urine and Saliva Biofluids: An Exploratory Study
title_full_unstemmed Interaction of Colloidal Gold Nanoparticles with Urine and Saliva Biofluids: An Exploratory Study
title_short Interaction of Colloidal Gold Nanoparticles with Urine and Saliva Biofluids: An Exploratory Study
title_sort interaction of colloidal gold nanoparticles with urine and saliva biofluids: an exploratory study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9785464/
https://www.ncbi.nlm.nih.gov/pubmed/36558287
http://dx.doi.org/10.3390/nano12244434
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