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EBF1 Negatively Regulates Brassinosteroid-Induced Apical Hook Development and Cell Elongation through Promoting BZR1 Degradation

Brassinosteroids (BRs) are a group of plant steroid hormones that play important roles in a wide range of developmental and physiological processes in plants. Transcription factors BRASSINOZALE-RESISTANT1 (BZR1) and its homologs are key components of BR signaling and integrate a wide range of intern...

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Autores principales: Zhao, Na, Zhao, Min, Wang, Lingyan, Han, Chao, Bai, Mingyi, Fan, Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9785488/
https://www.ncbi.nlm.nih.gov/pubmed/36555537
http://dx.doi.org/10.3390/ijms232415889
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author Zhao, Na
Zhao, Min
Wang, Lingyan
Han, Chao
Bai, Mingyi
Fan, Min
author_facet Zhao, Na
Zhao, Min
Wang, Lingyan
Han, Chao
Bai, Mingyi
Fan, Min
author_sort Zhao, Na
collection PubMed
description Brassinosteroids (BRs) are a group of plant steroid hormones that play important roles in a wide range of developmental and physiological processes in plants. Transcription factors BRASSINOZALE-RESISTANT1 (BZR1) and its homologs are key components of BR signaling and integrate a wide range of internal and environmental signals to coordinate plant growth and development. Although several E3 ligases have been reported to regulate the stability of BZR1, the molecular mechanism of BZR1 degradation remains unclear. Here, we reveal how a newly identified molecular mechanism underlying EBF1 directly regulates BZR1 protein stability via the 26S proteasome pathway, repressing BR function on regulating Arabidopsis apical hook development and hypocotyl elongation. BZR1 directly binds to the EBF1 gene promotor to reduce EBF1 expression. Furthermore, the genetic analysis shows that BZR1, EIN3 and PIF4 interdependently regulate plant apical hook development. Taken together, our data demonstrates that EBF1 is a negative regulator of the BR signaling pathway.
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spelling pubmed-97854882022-12-24 EBF1 Negatively Regulates Brassinosteroid-Induced Apical Hook Development and Cell Elongation through Promoting BZR1 Degradation Zhao, Na Zhao, Min Wang, Lingyan Han, Chao Bai, Mingyi Fan, Min Int J Mol Sci Article Brassinosteroids (BRs) are a group of plant steroid hormones that play important roles in a wide range of developmental and physiological processes in plants. Transcription factors BRASSINOZALE-RESISTANT1 (BZR1) and its homologs are key components of BR signaling and integrate a wide range of internal and environmental signals to coordinate plant growth and development. Although several E3 ligases have been reported to regulate the stability of BZR1, the molecular mechanism of BZR1 degradation remains unclear. Here, we reveal how a newly identified molecular mechanism underlying EBF1 directly regulates BZR1 protein stability via the 26S proteasome pathway, repressing BR function on regulating Arabidopsis apical hook development and hypocotyl elongation. BZR1 directly binds to the EBF1 gene promotor to reduce EBF1 expression. Furthermore, the genetic analysis shows that BZR1, EIN3 and PIF4 interdependently regulate plant apical hook development. Taken together, our data demonstrates that EBF1 is a negative regulator of the BR signaling pathway. MDPI 2022-12-14 /pmc/articles/PMC9785488/ /pubmed/36555537 http://dx.doi.org/10.3390/ijms232415889 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhao, Na
Zhao, Min
Wang, Lingyan
Han, Chao
Bai, Mingyi
Fan, Min
EBF1 Negatively Regulates Brassinosteroid-Induced Apical Hook Development and Cell Elongation through Promoting BZR1 Degradation
title EBF1 Negatively Regulates Brassinosteroid-Induced Apical Hook Development and Cell Elongation through Promoting BZR1 Degradation
title_full EBF1 Negatively Regulates Brassinosteroid-Induced Apical Hook Development and Cell Elongation through Promoting BZR1 Degradation
title_fullStr EBF1 Negatively Regulates Brassinosteroid-Induced Apical Hook Development and Cell Elongation through Promoting BZR1 Degradation
title_full_unstemmed EBF1 Negatively Regulates Brassinosteroid-Induced Apical Hook Development and Cell Elongation through Promoting BZR1 Degradation
title_short EBF1 Negatively Regulates Brassinosteroid-Induced Apical Hook Development and Cell Elongation through Promoting BZR1 Degradation
title_sort ebf1 negatively regulates brassinosteroid-induced apical hook development and cell elongation through promoting bzr1 degradation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9785488/
https://www.ncbi.nlm.nih.gov/pubmed/36555537
http://dx.doi.org/10.3390/ijms232415889
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