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Effects of Helicobacter pylori and Nitrate-Reducing Bacteria Coculture on Cells
Helicobacter pylori infection is an important risk factor for developing gastric cancer. However, only a few H. pylori-infected people develop gastric cancer. Thus, other risk factors aside from H. pylori infection may be involved in gastric cancer development. This study aimed to investigate whethe...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9785519/ https://www.ncbi.nlm.nih.gov/pubmed/36557748 http://dx.doi.org/10.3390/microorganisms10122495 |
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author | Ojima, Hinako Kuraoka, Sakiko Okanoue, Shyoutarou Okada, Hiroyuki Gotoh, Kazuyoshi Matsushita, Osamu Watanabe, Akari Yokota, Kenji |
author_facet | Ojima, Hinako Kuraoka, Sakiko Okanoue, Shyoutarou Okada, Hiroyuki Gotoh, Kazuyoshi Matsushita, Osamu Watanabe, Akari Yokota, Kenji |
author_sort | Ojima, Hinako |
collection | PubMed |
description | Helicobacter pylori infection is an important risk factor for developing gastric cancer. However, only a few H. pylori-infected people develop gastric cancer. Thus, other risk factors aside from H. pylori infection may be involved in gastric cancer development. This study aimed to investigate whether the nitrate-reducing bacteria isolated from patients with atrophic gastritis caused by H. pylori infection are risk factors for developing atrophic gastritis and gastric neoplasia. Nitrate-reducing bacteria were isolated from patients with atrophic gastritis caused by H. pylori infection. Among the isolated bacteria, Actinomyces oris, Actinomyces odontolyticus, Rothia dentocariosa, and Rothia mucilaginosa were used in the subsequent experiments. Cytokine inducibility was evaluated in monocytic cells, and mitogen-activated protein kinase (MAPK) activity and cell cycle were assessed in the gastric epithelial cells. The cytotoxicities and neutrophil-inducing abilities of the Actinomyces and Rothia species were enhanced when cocultured with H. pylori. Th1/Th2-related cytokines were also expressed, but their expression levels differed depending on the bacterial species. Moreover, H. pylori and Actinomyces activated MAPK (ERK and p38) and affected cell cycle progression. Some nitrate-reducing bacteria cocultured with H. pylori may promote inflammation and atrophy by inducing cytokine production. In addition, the MAPK activation and cell cycle progression caused by these bacteria can contribute to gastric cancer development. |
format | Online Article Text |
id | pubmed-9785519 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97855192022-12-24 Effects of Helicobacter pylori and Nitrate-Reducing Bacteria Coculture on Cells Ojima, Hinako Kuraoka, Sakiko Okanoue, Shyoutarou Okada, Hiroyuki Gotoh, Kazuyoshi Matsushita, Osamu Watanabe, Akari Yokota, Kenji Microorganisms Article Helicobacter pylori infection is an important risk factor for developing gastric cancer. However, only a few H. pylori-infected people develop gastric cancer. Thus, other risk factors aside from H. pylori infection may be involved in gastric cancer development. This study aimed to investigate whether the nitrate-reducing bacteria isolated from patients with atrophic gastritis caused by H. pylori infection are risk factors for developing atrophic gastritis and gastric neoplasia. Nitrate-reducing bacteria were isolated from patients with atrophic gastritis caused by H. pylori infection. Among the isolated bacteria, Actinomyces oris, Actinomyces odontolyticus, Rothia dentocariosa, and Rothia mucilaginosa were used in the subsequent experiments. Cytokine inducibility was evaluated in monocytic cells, and mitogen-activated protein kinase (MAPK) activity and cell cycle were assessed in the gastric epithelial cells. The cytotoxicities and neutrophil-inducing abilities of the Actinomyces and Rothia species were enhanced when cocultured with H. pylori. Th1/Th2-related cytokines were also expressed, but their expression levels differed depending on the bacterial species. Moreover, H. pylori and Actinomyces activated MAPK (ERK and p38) and affected cell cycle progression. Some nitrate-reducing bacteria cocultured with H. pylori may promote inflammation and atrophy by inducing cytokine production. In addition, the MAPK activation and cell cycle progression caused by these bacteria can contribute to gastric cancer development. MDPI 2022-12-16 /pmc/articles/PMC9785519/ /pubmed/36557748 http://dx.doi.org/10.3390/microorganisms10122495 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ojima, Hinako Kuraoka, Sakiko Okanoue, Shyoutarou Okada, Hiroyuki Gotoh, Kazuyoshi Matsushita, Osamu Watanabe, Akari Yokota, Kenji Effects of Helicobacter pylori and Nitrate-Reducing Bacteria Coculture on Cells |
title | Effects of Helicobacter pylori and Nitrate-Reducing Bacteria Coculture on Cells |
title_full | Effects of Helicobacter pylori and Nitrate-Reducing Bacteria Coculture on Cells |
title_fullStr | Effects of Helicobacter pylori and Nitrate-Reducing Bacteria Coculture on Cells |
title_full_unstemmed | Effects of Helicobacter pylori and Nitrate-Reducing Bacteria Coculture on Cells |
title_short | Effects of Helicobacter pylori and Nitrate-Reducing Bacteria Coculture on Cells |
title_sort | effects of helicobacter pylori and nitrate-reducing bacteria coculture on cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9785519/ https://www.ncbi.nlm.nih.gov/pubmed/36557748 http://dx.doi.org/10.3390/microorganisms10122495 |
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