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Effects of Helicobacter pylori and Nitrate-Reducing Bacteria Coculture on Cells

Helicobacter pylori infection is an important risk factor for developing gastric cancer. However, only a few H. pylori-infected people develop gastric cancer. Thus, other risk factors aside from H. pylori infection may be involved in gastric cancer development. This study aimed to investigate whethe...

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Autores principales: Ojima, Hinako, Kuraoka, Sakiko, Okanoue, Shyoutarou, Okada, Hiroyuki, Gotoh, Kazuyoshi, Matsushita, Osamu, Watanabe, Akari, Yokota, Kenji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9785519/
https://www.ncbi.nlm.nih.gov/pubmed/36557748
http://dx.doi.org/10.3390/microorganisms10122495
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author Ojima, Hinako
Kuraoka, Sakiko
Okanoue, Shyoutarou
Okada, Hiroyuki
Gotoh, Kazuyoshi
Matsushita, Osamu
Watanabe, Akari
Yokota, Kenji
author_facet Ojima, Hinako
Kuraoka, Sakiko
Okanoue, Shyoutarou
Okada, Hiroyuki
Gotoh, Kazuyoshi
Matsushita, Osamu
Watanabe, Akari
Yokota, Kenji
author_sort Ojima, Hinako
collection PubMed
description Helicobacter pylori infection is an important risk factor for developing gastric cancer. However, only a few H. pylori-infected people develop gastric cancer. Thus, other risk factors aside from H. pylori infection may be involved in gastric cancer development. This study aimed to investigate whether the nitrate-reducing bacteria isolated from patients with atrophic gastritis caused by H. pylori infection are risk factors for developing atrophic gastritis and gastric neoplasia. Nitrate-reducing bacteria were isolated from patients with atrophic gastritis caused by H. pylori infection. Among the isolated bacteria, Actinomyces oris, Actinomyces odontolyticus, Rothia dentocariosa, and Rothia mucilaginosa were used in the subsequent experiments. Cytokine inducibility was evaluated in monocytic cells, and mitogen-activated protein kinase (MAPK) activity and cell cycle were assessed in the gastric epithelial cells. The cytotoxicities and neutrophil-inducing abilities of the Actinomyces and Rothia species were enhanced when cocultured with H. pylori. Th1/Th2-related cytokines were also expressed, but their expression levels differed depending on the bacterial species. Moreover, H. pylori and Actinomyces activated MAPK (ERK and p38) and affected cell cycle progression. Some nitrate-reducing bacteria cocultured with H. pylori may promote inflammation and atrophy by inducing cytokine production. In addition, the MAPK activation and cell cycle progression caused by these bacteria can contribute to gastric cancer development.
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spelling pubmed-97855192022-12-24 Effects of Helicobacter pylori and Nitrate-Reducing Bacteria Coculture on Cells Ojima, Hinako Kuraoka, Sakiko Okanoue, Shyoutarou Okada, Hiroyuki Gotoh, Kazuyoshi Matsushita, Osamu Watanabe, Akari Yokota, Kenji Microorganisms Article Helicobacter pylori infection is an important risk factor for developing gastric cancer. However, only a few H. pylori-infected people develop gastric cancer. Thus, other risk factors aside from H. pylori infection may be involved in gastric cancer development. This study aimed to investigate whether the nitrate-reducing bacteria isolated from patients with atrophic gastritis caused by H. pylori infection are risk factors for developing atrophic gastritis and gastric neoplasia. Nitrate-reducing bacteria were isolated from patients with atrophic gastritis caused by H. pylori infection. Among the isolated bacteria, Actinomyces oris, Actinomyces odontolyticus, Rothia dentocariosa, and Rothia mucilaginosa were used in the subsequent experiments. Cytokine inducibility was evaluated in monocytic cells, and mitogen-activated protein kinase (MAPK) activity and cell cycle were assessed in the gastric epithelial cells. The cytotoxicities and neutrophil-inducing abilities of the Actinomyces and Rothia species were enhanced when cocultured with H. pylori. Th1/Th2-related cytokines were also expressed, but their expression levels differed depending on the bacterial species. Moreover, H. pylori and Actinomyces activated MAPK (ERK and p38) and affected cell cycle progression. Some nitrate-reducing bacteria cocultured with H. pylori may promote inflammation and atrophy by inducing cytokine production. In addition, the MAPK activation and cell cycle progression caused by these bacteria can contribute to gastric cancer development. MDPI 2022-12-16 /pmc/articles/PMC9785519/ /pubmed/36557748 http://dx.doi.org/10.3390/microorganisms10122495 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ojima, Hinako
Kuraoka, Sakiko
Okanoue, Shyoutarou
Okada, Hiroyuki
Gotoh, Kazuyoshi
Matsushita, Osamu
Watanabe, Akari
Yokota, Kenji
Effects of Helicobacter pylori and Nitrate-Reducing Bacteria Coculture on Cells
title Effects of Helicobacter pylori and Nitrate-Reducing Bacteria Coculture on Cells
title_full Effects of Helicobacter pylori and Nitrate-Reducing Bacteria Coculture on Cells
title_fullStr Effects of Helicobacter pylori and Nitrate-Reducing Bacteria Coculture on Cells
title_full_unstemmed Effects of Helicobacter pylori and Nitrate-Reducing Bacteria Coculture on Cells
title_short Effects of Helicobacter pylori and Nitrate-Reducing Bacteria Coculture on Cells
title_sort effects of helicobacter pylori and nitrate-reducing bacteria coculture on cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9785519/
https://www.ncbi.nlm.nih.gov/pubmed/36557748
http://dx.doi.org/10.3390/microorganisms10122495
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