Cargando…

Fabrication of Curcumin Diethyl γ-Aminobutyrate-Loaded Chitosan-Coated Magnetic Nanocarriers for Improvement of Cytotoxicity against Breast Cancer Cells

This study shows the effectiveness of magnetic-guide targeting in the delivery of curcumin diethyl γ-aminobutyrate (CUR-2GE), a prodrug of curcumin (CUR) previously synthesized to overcome unfavorable physicochemical properties of CUR. In this study, chitosan (Ch)-coated iron oxide nanoparticles (Ch...

Descripción completa

Detalles Bibliográficos
Autores principales: Hansapaiboon, Supakarn, Bulatao, Bryan Paul, Sorasitthiyanukarn, Feuangthit Niyamissara, Jantaratana, Pongsakorn, Nalinratana, Nonthaneth, Vajragupta, Opa, Rojsitthisak, Pranee, Rojsitthisak, Pornchai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9785553/
https://www.ncbi.nlm.nih.gov/pubmed/36559930
http://dx.doi.org/10.3390/polym14245563
_version_ 1784858076930637824
author Hansapaiboon, Supakarn
Bulatao, Bryan Paul
Sorasitthiyanukarn, Feuangthit Niyamissara
Jantaratana, Pongsakorn
Nalinratana, Nonthaneth
Vajragupta, Opa
Rojsitthisak, Pranee
Rojsitthisak, Pornchai
author_facet Hansapaiboon, Supakarn
Bulatao, Bryan Paul
Sorasitthiyanukarn, Feuangthit Niyamissara
Jantaratana, Pongsakorn
Nalinratana, Nonthaneth
Vajragupta, Opa
Rojsitthisak, Pranee
Rojsitthisak, Pornchai
author_sort Hansapaiboon, Supakarn
collection PubMed
description This study shows the effectiveness of magnetic-guide targeting in the delivery of curcumin diethyl γ-aminobutyrate (CUR-2GE), a prodrug of curcumin (CUR) previously synthesized to overcome unfavorable physicochemical properties of CUR. In this study, chitosan (Ch)-coated iron oxide nanoparticles (Ch-IONPs) were fabricated and optimized using Box–Behnken design-based response surface methodology for delivery of CUR-2GE. Ch was used as a coating material on the nanoparticle surface to avoid aggregation. The optimized condition for preparing Ch-IONPs consisted of using 4 mg Ch fabricated at pH 11 under a reaction temperature of 85 °C. The optimized Ch-IONPs were successfully loaded with CUR-2GE with sufficient loading capacity (1.72 ± 0.01%) and encapsulation efficiency (94.9 ± 0.8%). The obtained CUR-2GE-loaded Ch-IONPs (CUR-2GE-Ch-IONPs) exhibited desirable characteristics including a particle size of less than 50 nm based on TEM images, superparamagnetic property, highly crystalline IONP core, sufficient stability, and sustained-release profile. In the presence of permanent magnets, CUR-2GE-Ch-IONPs significantly increased cellular uptake and cytotoxicity toward MDA-MB-231 with a 12-fold increase in potency compared to free CUR-2GE, indicating the potential of magnetic-field assisted delivery of CUR-2GE-Ch-IONPs for the treatment of triple-negative breast cancer.
format Online
Article
Text
id pubmed-9785553
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-97855532022-12-24 Fabrication of Curcumin Diethyl γ-Aminobutyrate-Loaded Chitosan-Coated Magnetic Nanocarriers for Improvement of Cytotoxicity against Breast Cancer Cells Hansapaiboon, Supakarn Bulatao, Bryan Paul Sorasitthiyanukarn, Feuangthit Niyamissara Jantaratana, Pongsakorn Nalinratana, Nonthaneth Vajragupta, Opa Rojsitthisak, Pranee Rojsitthisak, Pornchai Polymers (Basel) Article This study shows the effectiveness of magnetic-guide targeting in the delivery of curcumin diethyl γ-aminobutyrate (CUR-2GE), a prodrug of curcumin (CUR) previously synthesized to overcome unfavorable physicochemical properties of CUR. In this study, chitosan (Ch)-coated iron oxide nanoparticles (Ch-IONPs) were fabricated and optimized using Box–Behnken design-based response surface methodology for delivery of CUR-2GE. Ch was used as a coating material on the nanoparticle surface to avoid aggregation. The optimized condition for preparing Ch-IONPs consisted of using 4 mg Ch fabricated at pH 11 under a reaction temperature of 85 °C. The optimized Ch-IONPs were successfully loaded with CUR-2GE with sufficient loading capacity (1.72 ± 0.01%) and encapsulation efficiency (94.9 ± 0.8%). The obtained CUR-2GE-loaded Ch-IONPs (CUR-2GE-Ch-IONPs) exhibited desirable characteristics including a particle size of less than 50 nm based on TEM images, superparamagnetic property, highly crystalline IONP core, sufficient stability, and sustained-release profile. In the presence of permanent magnets, CUR-2GE-Ch-IONPs significantly increased cellular uptake and cytotoxicity toward MDA-MB-231 with a 12-fold increase in potency compared to free CUR-2GE, indicating the potential of magnetic-field assisted delivery of CUR-2GE-Ch-IONPs for the treatment of triple-negative breast cancer. MDPI 2022-12-19 /pmc/articles/PMC9785553/ /pubmed/36559930 http://dx.doi.org/10.3390/polym14245563 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hansapaiboon, Supakarn
Bulatao, Bryan Paul
Sorasitthiyanukarn, Feuangthit Niyamissara
Jantaratana, Pongsakorn
Nalinratana, Nonthaneth
Vajragupta, Opa
Rojsitthisak, Pranee
Rojsitthisak, Pornchai
Fabrication of Curcumin Diethyl γ-Aminobutyrate-Loaded Chitosan-Coated Magnetic Nanocarriers for Improvement of Cytotoxicity against Breast Cancer Cells
title Fabrication of Curcumin Diethyl γ-Aminobutyrate-Loaded Chitosan-Coated Magnetic Nanocarriers for Improvement of Cytotoxicity against Breast Cancer Cells
title_full Fabrication of Curcumin Diethyl γ-Aminobutyrate-Loaded Chitosan-Coated Magnetic Nanocarriers for Improvement of Cytotoxicity against Breast Cancer Cells
title_fullStr Fabrication of Curcumin Diethyl γ-Aminobutyrate-Loaded Chitosan-Coated Magnetic Nanocarriers for Improvement of Cytotoxicity against Breast Cancer Cells
title_full_unstemmed Fabrication of Curcumin Diethyl γ-Aminobutyrate-Loaded Chitosan-Coated Magnetic Nanocarriers for Improvement of Cytotoxicity against Breast Cancer Cells
title_short Fabrication of Curcumin Diethyl γ-Aminobutyrate-Loaded Chitosan-Coated Magnetic Nanocarriers for Improvement of Cytotoxicity against Breast Cancer Cells
title_sort fabrication of curcumin diethyl γ-aminobutyrate-loaded chitosan-coated magnetic nanocarriers for improvement of cytotoxicity against breast cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9785553/
https://www.ncbi.nlm.nih.gov/pubmed/36559930
http://dx.doi.org/10.3390/polym14245563
work_keys_str_mv AT hansapaiboonsupakarn fabricationofcurcumindiethylgaminobutyrateloadedchitosancoatedmagneticnanocarriersforimprovementofcytotoxicityagainstbreastcancercells
AT bulataobryanpaul fabricationofcurcumindiethylgaminobutyrateloadedchitosancoatedmagneticnanocarriersforimprovementofcytotoxicityagainstbreastcancercells
AT sorasitthiyanukarnfeuangthitniyamissara fabricationofcurcumindiethylgaminobutyrateloadedchitosancoatedmagneticnanocarriersforimprovementofcytotoxicityagainstbreastcancercells
AT jantaratanapongsakorn fabricationofcurcumindiethylgaminobutyrateloadedchitosancoatedmagneticnanocarriersforimprovementofcytotoxicityagainstbreastcancercells
AT nalinratananonthaneth fabricationofcurcumindiethylgaminobutyrateloadedchitosancoatedmagneticnanocarriersforimprovementofcytotoxicityagainstbreastcancercells
AT vajraguptaopa fabricationofcurcumindiethylgaminobutyrateloadedchitosancoatedmagneticnanocarriersforimprovementofcytotoxicityagainstbreastcancercells
AT rojsitthisakpranee fabricationofcurcumindiethylgaminobutyrateloadedchitosancoatedmagneticnanocarriersforimprovementofcytotoxicityagainstbreastcancercells
AT rojsitthisakpornchai fabricationofcurcumindiethylgaminobutyrateloadedchitosancoatedmagneticnanocarriersforimprovementofcytotoxicityagainstbreastcancercells