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Development of an Immunofluorescent Capillary Sensor for the Detection of Zearalenone Mycotoxin

A capillary-based immunofluorescence sensor was developed and incorporated in a flow injection analysis system. The light-guiding capillary was illuminated axially by a 473 nm/5 mW solid state laser through a tailored optofluidic connector. High sensitivity of the system was achieved by efficiently...

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Autores principales: Majer-Baranyi, Krisztina, Barócsi, Attila, Gádoros, Patrik, Kocsányi, László, Székács, András, Adányi, Nóra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9785567/
https://www.ncbi.nlm.nih.gov/pubmed/36548763
http://dx.doi.org/10.3390/toxins14120866
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author Majer-Baranyi, Krisztina
Barócsi, Attila
Gádoros, Patrik
Kocsányi, László
Székács, András
Adányi, Nóra
author_facet Majer-Baranyi, Krisztina
Barócsi, Attila
Gádoros, Patrik
Kocsányi, László
Székács, András
Adányi, Nóra
author_sort Majer-Baranyi, Krisztina
collection PubMed
description A capillary-based immunofluorescence sensor was developed and incorporated in a flow injection analysis system. The light-guiding capillary was illuminated axially by a 473 nm/5 mW solid state laser through a tailored optofluidic connector. High sensitivity of the system was achieved by efficiently collecting and detecting the non-guided fluorescence signal scattered out along the wall of the capillary. The excitation was highly suppressed with bandpass and dichroic filters by simultaneously exploiting the guiding effect inside the capillary. The glass capillary used as a measuring cell was silanized in liquid phase by 3-aminopropyltriethoxysilane (APTS), and the biomolecules were immobilized using glutaraldehyde inside the capillary. The applicability of the developed system was tested with a bovine serum albumin (BSA)—anti-BSA-IgG model-molecule pair, using a fluorescently labeled secondary antibody. Based on the results of the BSA–anti-BSA experiments, a similar setup using a primary antibody specific for zearalenone (ZON) was established, and a competitive fluorescence measurement system was developed for quantitative determination of ZON. For the measurements, 20 µg/mL ZON-BSA conjugate was immobilized in the capillary, and a 1:2500 dilution of the primary antibody stock solution and a 2 µg/mL secondary antibody solution were set. The developed capillary-based immunosensor allowed a limit of detection (LOD) of 0.003 ng/mL and a limit of quantification (LOQ) of 0.007 ng/mL for ZON in the competitive immunosensor setup, with a dynamic detection range of 0.01–10 ng/mL ZON concentrations.
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spelling pubmed-97855672022-12-24 Development of an Immunofluorescent Capillary Sensor for the Detection of Zearalenone Mycotoxin Majer-Baranyi, Krisztina Barócsi, Attila Gádoros, Patrik Kocsányi, László Székács, András Adányi, Nóra Toxins (Basel) Article A capillary-based immunofluorescence sensor was developed and incorporated in a flow injection analysis system. The light-guiding capillary was illuminated axially by a 473 nm/5 mW solid state laser through a tailored optofluidic connector. High sensitivity of the system was achieved by efficiently collecting and detecting the non-guided fluorescence signal scattered out along the wall of the capillary. The excitation was highly suppressed with bandpass and dichroic filters by simultaneously exploiting the guiding effect inside the capillary. The glass capillary used as a measuring cell was silanized in liquid phase by 3-aminopropyltriethoxysilane (APTS), and the biomolecules were immobilized using glutaraldehyde inside the capillary. The applicability of the developed system was tested with a bovine serum albumin (BSA)—anti-BSA-IgG model-molecule pair, using a fluorescently labeled secondary antibody. Based on the results of the BSA–anti-BSA experiments, a similar setup using a primary antibody specific for zearalenone (ZON) was established, and a competitive fluorescence measurement system was developed for quantitative determination of ZON. For the measurements, 20 µg/mL ZON-BSA conjugate was immobilized in the capillary, and a 1:2500 dilution of the primary antibody stock solution and a 2 µg/mL secondary antibody solution were set. The developed capillary-based immunosensor allowed a limit of detection (LOD) of 0.003 ng/mL and a limit of quantification (LOQ) of 0.007 ng/mL for ZON in the competitive immunosensor setup, with a dynamic detection range of 0.01–10 ng/mL ZON concentrations. MDPI 2022-12-09 /pmc/articles/PMC9785567/ /pubmed/36548763 http://dx.doi.org/10.3390/toxins14120866 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Majer-Baranyi, Krisztina
Barócsi, Attila
Gádoros, Patrik
Kocsányi, László
Székács, András
Adányi, Nóra
Development of an Immunofluorescent Capillary Sensor for the Detection of Zearalenone Mycotoxin
title Development of an Immunofluorescent Capillary Sensor for the Detection of Zearalenone Mycotoxin
title_full Development of an Immunofluorescent Capillary Sensor for the Detection of Zearalenone Mycotoxin
title_fullStr Development of an Immunofluorescent Capillary Sensor for the Detection of Zearalenone Mycotoxin
title_full_unstemmed Development of an Immunofluorescent Capillary Sensor for the Detection of Zearalenone Mycotoxin
title_short Development of an Immunofluorescent Capillary Sensor for the Detection of Zearalenone Mycotoxin
title_sort development of an immunofluorescent capillary sensor for the detection of zearalenone mycotoxin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9785567/
https://www.ncbi.nlm.nih.gov/pubmed/36548763
http://dx.doi.org/10.3390/toxins14120866
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