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Impact of Fecal Microbiota Transplantation on Gut Bacterial Bile Acid Metabolism in Humans
Fecal microbiota transplantation (FMT) is a promising therapeutic modality for the treatment and prevention of metabolic disease. We previously conducted a double-blind, randomized, placebo-controlled pilot trial of FMT in obese metabolically healthy patients in which we found that FMT enhanced gut...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9785599/ https://www.ncbi.nlm.nih.gov/pubmed/36558359 http://dx.doi.org/10.3390/nu14245200 |
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author | Bustamante, Jessica-Miranda Dawson, Tyson Loeffler, Caitlin Marfori, Zara Marchesi, Julian R. Mullish, Benjamin H. Thompson, Christopher C. Crandall, Keith A. Rahnavard, Ali Allegretti, Jessica R. Cummings, Bethany P. |
author_facet | Bustamante, Jessica-Miranda Dawson, Tyson Loeffler, Caitlin Marfori, Zara Marchesi, Julian R. Mullish, Benjamin H. Thompson, Christopher C. Crandall, Keith A. Rahnavard, Ali Allegretti, Jessica R. Cummings, Bethany P. |
author_sort | Bustamante, Jessica-Miranda |
collection | PubMed |
description | Fecal microbiota transplantation (FMT) is a promising therapeutic modality for the treatment and prevention of metabolic disease. We previously conducted a double-blind, randomized, placebo-controlled pilot trial of FMT in obese metabolically healthy patients in which we found that FMT enhanced gut bacterial bile acid metabolism and delayed the development of impaired glucose tolerance relative to the placebo control group. Therefore, we conducted a secondary analysis of fecal samples collected from these patients to assess the potential gut microbial species contributing to the effect of FMT to improve metabolic health and increase gut bacterial bile acid metabolism. Fecal samples collected at baseline and after 4 weeks of FMT or placebo treatment underwent shotgun metagenomic analysis. Ultra-high-performance liquid chromatography-mass spectrometry was used to profile fecal bile acids. FMT-enriched bacteria that have been implicated in gut bile acid metabolism included Desulfovibrio fairfieldensis and Clostridium hylemonae. To identify candidate bacteria involved in gut microbial bile acid metabolism, we assessed correlations between bacterial species abundance and bile acid profile, with a focus on bile acid products of gut bacterial metabolism. Bacteroides ovatus and Phocaeicola dorei were positively correlated with unconjugated bile acids. Bifidobacterium adolescentis, Collinsella aerofaciens, and Faecalibacterium prausnitzii were positively correlated with secondary bile acids. Together, these data identify several candidate bacteria that may contribute to the metabolic benefits of FMT and gut bacterial bile acid metabolism that requires further functional validation. |
format | Online Article Text |
id | pubmed-9785599 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97855992022-12-24 Impact of Fecal Microbiota Transplantation on Gut Bacterial Bile Acid Metabolism in Humans Bustamante, Jessica-Miranda Dawson, Tyson Loeffler, Caitlin Marfori, Zara Marchesi, Julian R. Mullish, Benjamin H. Thompson, Christopher C. Crandall, Keith A. Rahnavard, Ali Allegretti, Jessica R. Cummings, Bethany P. Nutrients Article Fecal microbiota transplantation (FMT) is a promising therapeutic modality for the treatment and prevention of metabolic disease. We previously conducted a double-blind, randomized, placebo-controlled pilot trial of FMT in obese metabolically healthy patients in which we found that FMT enhanced gut bacterial bile acid metabolism and delayed the development of impaired glucose tolerance relative to the placebo control group. Therefore, we conducted a secondary analysis of fecal samples collected from these patients to assess the potential gut microbial species contributing to the effect of FMT to improve metabolic health and increase gut bacterial bile acid metabolism. Fecal samples collected at baseline and after 4 weeks of FMT or placebo treatment underwent shotgun metagenomic analysis. Ultra-high-performance liquid chromatography-mass spectrometry was used to profile fecal bile acids. FMT-enriched bacteria that have been implicated in gut bile acid metabolism included Desulfovibrio fairfieldensis and Clostridium hylemonae. To identify candidate bacteria involved in gut microbial bile acid metabolism, we assessed correlations between bacterial species abundance and bile acid profile, with a focus on bile acid products of gut bacterial metabolism. Bacteroides ovatus and Phocaeicola dorei were positively correlated with unconjugated bile acids. Bifidobacterium adolescentis, Collinsella aerofaciens, and Faecalibacterium prausnitzii were positively correlated with secondary bile acids. Together, these data identify several candidate bacteria that may contribute to the metabolic benefits of FMT and gut bacterial bile acid metabolism that requires further functional validation. MDPI 2022-12-07 /pmc/articles/PMC9785599/ /pubmed/36558359 http://dx.doi.org/10.3390/nu14245200 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bustamante, Jessica-Miranda Dawson, Tyson Loeffler, Caitlin Marfori, Zara Marchesi, Julian R. Mullish, Benjamin H. Thompson, Christopher C. Crandall, Keith A. Rahnavard, Ali Allegretti, Jessica R. Cummings, Bethany P. Impact of Fecal Microbiota Transplantation on Gut Bacterial Bile Acid Metabolism in Humans |
title | Impact of Fecal Microbiota Transplantation on Gut Bacterial Bile Acid Metabolism in Humans |
title_full | Impact of Fecal Microbiota Transplantation on Gut Bacterial Bile Acid Metabolism in Humans |
title_fullStr | Impact of Fecal Microbiota Transplantation on Gut Bacterial Bile Acid Metabolism in Humans |
title_full_unstemmed | Impact of Fecal Microbiota Transplantation on Gut Bacterial Bile Acid Metabolism in Humans |
title_short | Impact of Fecal Microbiota Transplantation on Gut Bacterial Bile Acid Metabolism in Humans |
title_sort | impact of fecal microbiota transplantation on gut bacterial bile acid metabolism in humans |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9785599/ https://www.ncbi.nlm.nih.gov/pubmed/36558359 http://dx.doi.org/10.3390/nu14245200 |
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