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Impact of Fecal Microbiota Transplantation on Gut Bacterial Bile Acid Metabolism in Humans

Fecal microbiota transplantation (FMT) is a promising therapeutic modality for the treatment and prevention of metabolic disease. We previously conducted a double-blind, randomized, placebo-controlled pilot trial of FMT in obese metabolically healthy patients in which we found that FMT enhanced gut...

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Autores principales: Bustamante, Jessica-Miranda, Dawson, Tyson, Loeffler, Caitlin, Marfori, Zara, Marchesi, Julian R., Mullish, Benjamin H., Thompson, Christopher C., Crandall, Keith A., Rahnavard, Ali, Allegretti, Jessica R., Cummings, Bethany P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9785599/
https://www.ncbi.nlm.nih.gov/pubmed/36558359
http://dx.doi.org/10.3390/nu14245200
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author Bustamante, Jessica-Miranda
Dawson, Tyson
Loeffler, Caitlin
Marfori, Zara
Marchesi, Julian R.
Mullish, Benjamin H.
Thompson, Christopher C.
Crandall, Keith A.
Rahnavard, Ali
Allegretti, Jessica R.
Cummings, Bethany P.
author_facet Bustamante, Jessica-Miranda
Dawson, Tyson
Loeffler, Caitlin
Marfori, Zara
Marchesi, Julian R.
Mullish, Benjamin H.
Thompson, Christopher C.
Crandall, Keith A.
Rahnavard, Ali
Allegretti, Jessica R.
Cummings, Bethany P.
author_sort Bustamante, Jessica-Miranda
collection PubMed
description Fecal microbiota transplantation (FMT) is a promising therapeutic modality for the treatment and prevention of metabolic disease. We previously conducted a double-blind, randomized, placebo-controlled pilot trial of FMT in obese metabolically healthy patients in which we found that FMT enhanced gut bacterial bile acid metabolism and delayed the development of impaired glucose tolerance relative to the placebo control group. Therefore, we conducted a secondary analysis of fecal samples collected from these patients to assess the potential gut microbial species contributing to the effect of FMT to improve metabolic health and increase gut bacterial bile acid metabolism. Fecal samples collected at baseline and after 4 weeks of FMT or placebo treatment underwent shotgun metagenomic analysis. Ultra-high-performance liquid chromatography-mass spectrometry was used to profile fecal bile acids. FMT-enriched bacteria that have been implicated in gut bile acid metabolism included Desulfovibrio fairfieldensis and Clostridium hylemonae. To identify candidate bacteria involved in gut microbial bile acid metabolism, we assessed correlations between bacterial species abundance and bile acid profile, with a focus on bile acid products of gut bacterial metabolism. Bacteroides ovatus and Phocaeicola dorei were positively correlated with unconjugated bile acids. Bifidobacterium adolescentis, Collinsella aerofaciens, and Faecalibacterium prausnitzii were positively correlated with secondary bile acids. Together, these data identify several candidate bacteria that may contribute to the metabolic benefits of FMT and gut bacterial bile acid metabolism that requires further functional validation.
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spelling pubmed-97855992022-12-24 Impact of Fecal Microbiota Transplantation on Gut Bacterial Bile Acid Metabolism in Humans Bustamante, Jessica-Miranda Dawson, Tyson Loeffler, Caitlin Marfori, Zara Marchesi, Julian R. Mullish, Benjamin H. Thompson, Christopher C. Crandall, Keith A. Rahnavard, Ali Allegretti, Jessica R. Cummings, Bethany P. Nutrients Article Fecal microbiota transplantation (FMT) is a promising therapeutic modality for the treatment and prevention of metabolic disease. We previously conducted a double-blind, randomized, placebo-controlled pilot trial of FMT in obese metabolically healthy patients in which we found that FMT enhanced gut bacterial bile acid metabolism and delayed the development of impaired glucose tolerance relative to the placebo control group. Therefore, we conducted a secondary analysis of fecal samples collected from these patients to assess the potential gut microbial species contributing to the effect of FMT to improve metabolic health and increase gut bacterial bile acid metabolism. Fecal samples collected at baseline and after 4 weeks of FMT or placebo treatment underwent shotgun metagenomic analysis. Ultra-high-performance liquid chromatography-mass spectrometry was used to profile fecal bile acids. FMT-enriched bacteria that have been implicated in gut bile acid metabolism included Desulfovibrio fairfieldensis and Clostridium hylemonae. To identify candidate bacteria involved in gut microbial bile acid metabolism, we assessed correlations between bacterial species abundance and bile acid profile, with a focus on bile acid products of gut bacterial metabolism. Bacteroides ovatus and Phocaeicola dorei were positively correlated with unconjugated bile acids. Bifidobacterium adolescentis, Collinsella aerofaciens, and Faecalibacterium prausnitzii were positively correlated with secondary bile acids. Together, these data identify several candidate bacteria that may contribute to the metabolic benefits of FMT and gut bacterial bile acid metabolism that requires further functional validation. MDPI 2022-12-07 /pmc/articles/PMC9785599/ /pubmed/36558359 http://dx.doi.org/10.3390/nu14245200 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bustamante, Jessica-Miranda
Dawson, Tyson
Loeffler, Caitlin
Marfori, Zara
Marchesi, Julian R.
Mullish, Benjamin H.
Thompson, Christopher C.
Crandall, Keith A.
Rahnavard, Ali
Allegretti, Jessica R.
Cummings, Bethany P.
Impact of Fecal Microbiota Transplantation on Gut Bacterial Bile Acid Metabolism in Humans
title Impact of Fecal Microbiota Transplantation on Gut Bacterial Bile Acid Metabolism in Humans
title_full Impact of Fecal Microbiota Transplantation on Gut Bacterial Bile Acid Metabolism in Humans
title_fullStr Impact of Fecal Microbiota Transplantation on Gut Bacterial Bile Acid Metabolism in Humans
title_full_unstemmed Impact of Fecal Microbiota Transplantation on Gut Bacterial Bile Acid Metabolism in Humans
title_short Impact of Fecal Microbiota Transplantation on Gut Bacterial Bile Acid Metabolism in Humans
title_sort impact of fecal microbiota transplantation on gut bacterial bile acid metabolism in humans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9785599/
https://www.ncbi.nlm.nih.gov/pubmed/36558359
http://dx.doi.org/10.3390/nu14245200
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