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Exploring Freeze-Drying as Strategy to Enhance Viability of Faecalibacterium duncaniae DSM 17677 upon Aerobic Storage and Gastrointestinal Conditions

Faecalibacterium duncaniae is an intestinal commensal bacterium proposed as a next-generation probiotic due to its promising outcomes in the treatment and prevention of several human diseases, which demonstrate its multiple contributions to the host’s health. However, its strict anaerobic nature has...

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Autores principales: Machado, Daniela, Domingos, Melany, Barbosa, Joana Cristina, Almeida, Diana, Andrade, José Carlos, Freitas, Ana Cristina, Gomes, Ana Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9785611/
https://www.ncbi.nlm.nih.gov/pubmed/36559229
http://dx.doi.org/10.3390/pharmaceutics14122735
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author Machado, Daniela
Domingos, Melany
Barbosa, Joana Cristina
Almeida, Diana
Andrade, José Carlos
Freitas, Ana Cristina
Gomes, Ana Maria
author_facet Machado, Daniela
Domingos, Melany
Barbosa, Joana Cristina
Almeida, Diana
Andrade, José Carlos
Freitas, Ana Cristina
Gomes, Ana Maria
author_sort Machado, Daniela
collection PubMed
description Faecalibacterium duncaniae is an intestinal commensal bacterium proposed as a next-generation probiotic due to its promising outcomes in the treatment and prevention of several human diseases, which demonstrate its multiple contributions to the host’s health. However, its strict anaerobic nature has created several hurdles in the development of functional foods, nutraceuticals, and biotherapeutic products. Herein, we explored freeze-dried formulations containing prebiotics, cryoprotectants, and antioxidant agents as a technological strategy to enhance the viability of F. duncaniae DSM 17677 upon aerobic storage and gastrointestinal tract conditions. Our results indicate that freeze-dried F. duncaniae in a matrix containing inulin, sucrose, cysteine, and riboflavin survived at levels higher than 10(6) CFU/g and around 10(5) CFU/g after 1 and 4 days of aerobic storage at room temperature, respectively. Thus, the freeze-dried formulation with inulin, sucrose, cysteine, and riboflavin presents as a protective strategy to improve F. duncaniae viability under aerobic environments. Nevertheless, incorporation of a suitable coating aimed at protecting F. duncaniae against the detrimental gastrointestinal passage effects is urgently required, given its high susceptibility to extreme acidic pH values and bile.
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spelling pubmed-97856112022-12-24 Exploring Freeze-Drying as Strategy to Enhance Viability of Faecalibacterium duncaniae DSM 17677 upon Aerobic Storage and Gastrointestinal Conditions Machado, Daniela Domingos, Melany Barbosa, Joana Cristina Almeida, Diana Andrade, José Carlos Freitas, Ana Cristina Gomes, Ana Maria Pharmaceutics Article Faecalibacterium duncaniae is an intestinal commensal bacterium proposed as a next-generation probiotic due to its promising outcomes in the treatment and prevention of several human diseases, which demonstrate its multiple contributions to the host’s health. However, its strict anaerobic nature has created several hurdles in the development of functional foods, nutraceuticals, and biotherapeutic products. Herein, we explored freeze-dried formulations containing prebiotics, cryoprotectants, and antioxidant agents as a technological strategy to enhance the viability of F. duncaniae DSM 17677 upon aerobic storage and gastrointestinal tract conditions. Our results indicate that freeze-dried F. duncaniae in a matrix containing inulin, sucrose, cysteine, and riboflavin survived at levels higher than 10(6) CFU/g and around 10(5) CFU/g after 1 and 4 days of aerobic storage at room temperature, respectively. Thus, the freeze-dried formulation with inulin, sucrose, cysteine, and riboflavin presents as a protective strategy to improve F. duncaniae viability under aerobic environments. Nevertheless, incorporation of a suitable coating aimed at protecting F. duncaniae against the detrimental gastrointestinal passage effects is urgently required, given its high susceptibility to extreme acidic pH values and bile. MDPI 2022-12-07 /pmc/articles/PMC9785611/ /pubmed/36559229 http://dx.doi.org/10.3390/pharmaceutics14122735 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Machado, Daniela
Domingos, Melany
Barbosa, Joana Cristina
Almeida, Diana
Andrade, José Carlos
Freitas, Ana Cristina
Gomes, Ana Maria
Exploring Freeze-Drying as Strategy to Enhance Viability of Faecalibacterium duncaniae DSM 17677 upon Aerobic Storage and Gastrointestinal Conditions
title Exploring Freeze-Drying as Strategy to Enhance Viability of Faecalibacterium duncaniae DSM 17677 upon Aerobic Storage and Gastrointestinal Conditions
title_full Exploring Freeze-Drying as Strategy to Enhance Viability of Faecalibacterium duncaniae DSM 17677 upon Aerobic Storage and Gastrointestinal Conditions
title_fullStr Exploring Freeze-Drying as Strategy to Enhance Viability of Faecalibacterium duncaniae DSM 17677 upon Aerobic Storage and Gastrointestinal Conditions
title_full_unstemmed Exploring Freeze-Drying as Strategy to Enhance Viability of Faecalibacterium duncaniae DSM 17677 upon Aerobic Storage and Gastrointestinal Conditions
title_short Exploring Freeze-Drying as Strategy to Enhance Viability of Faecalibacterium duncaniae DSM 17677 upon Aerobic Storage and Gastrointestinal Conditions
title_sort exploring freeze-drying as strategy to enhance viability of faecalibacterium duncaniae dsm 17677 upon aerobic storage and gastrointestinal conditions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9785611/
https://www.ncbi.nlm.nih.gov/pubmed/36559229
http://dx.doi.org/10.3390/pharmaceutics14122735
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