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Stability of Non-Ionic Surfactant Vesicles Loaded with Rifamycin S
These days, the eradication of bacterial infections is more difficult due to the mechanism of resistance that bacteria have developed towards traditional antibiotics. One of the medical strategies used against bacteria is the therapy with drug delivery systems. Non-ionic vesicles are nanomaterials w...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9785864/ https://www.ncbi.nlm.nih.gov/pubmed/36559121 http://dx.doi.org/10.3390/pharmaceutics14122626 |
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author | Marchianò, Verdiana Matos, Maria Marcet, Ismael Cabal, Maria Paz Gutiérrez, Gemma Blanco-López, Maria Carmen |
author_facet | Marchianò, Verdiana Matos, Maria Marcet, Ismael Cabal, Maria Paz Gutiérrez, Gemma Blanco-López, Maria Carmen |
author_sort | Marchianò, Verdiana |
collection | PubMed |
description | These days, the eradication of bacterial infections is more difficult due to the mechanism of resistance that bacteria have developed towards traditional antibiotics. One of the medical strategies used against bacteria is the therapy with drug delivery systems. Non-ionic vesicles are nanomaterials with good characteristics for encapsulating drugs, due to their bioavailability and biodegradability, which allow the drugs to reach the specific target and reduce their side effects. In this work, the antibiotic Rifamycin S was encapsulated. The rifamycin antibiotics family has been widely used against Mycobacterium tuberculosis, but recent studies have also shown that rifamycin S and rifampicin derivatives have bactericidal activity against Staphylococcus epidermidis and Staphylococcus aureus. In this work, a strain of S. aureus was selected to study the antimicrobial activity through Minimum Inhibitory Concentration (MIC) assay. Three formulations of niosomes were prepared using the thin film hydration method by varying the composition of the aqueous phase, which included MilliQ water, glycerol solution, or PEG400 solution. Niosomes with a rifamycin S concentration of 0.13 μg/g were satisfactorily prepared. Nanovesicles with larger size and higher encapsulation efficiency (EE) were obtained when using glycerol and PEG400 in the aqueous media. Our results showed that niosomes consisting of an aqueous glycerol solution have higher stability and EE across a diversity of temperatures and pHs, and a lower MIC of rifamycin S against S. aureus. |
format | Online Article Text |
id | pubmed-9785864 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97858642022-12-24 Stability of Non-Ionic Surfactant Vesicles Loaded with Rifamycin S Marchianò, Verdiana Matos, Maria Marcet, Ismael Cabal, Maria Paz Gutiérrez, Gemma Blanco-López, Maria Carmen Pharmaceutics Article These days, the eradication of bacterial infections is more difficult due to the mechanism of resistance that bacteria have developed towards traditional antibiotics. One of the medical strategies used against bacteria is the therapy with drug delivery systems. Non-ionic vesicles are nanomaterials with good characteristics for encapsulating drugs, due to their bioavailability and biodegradability, which allow the drugs to reach the specific target and reduce their side effects. In this work, the antibiotic Rifamycin S was encapsulated. The rifamycin antibiotics family has been widely used against Mycobacterium tuberculosis, but recent studies have also shown that rifamycin S and rifampicin derivatives have bactericidal activity against Staphylococcus epidermidis and Staphylococcus aureus. In this work, a strain of S. aureus was selected to study the antimicrobial activity through Minimum Inhibitory Concentration (MIC) assay. Three formulations of niosomes were prepared using the thin film hydration method by varying the composition of the aqueous phase, which included MilliQ water, glycerol solution, or PEG400 solution. Niosomes with a rifamycin S concentration of 0.13 μg/g were satisfactorily prepared. Nanovesicles with larger size and higher encapsulation efficiency (EE) were obtained when using glycerol and PEG400 in the aqueous media. Our results showed that niosomes consisting of an aqueous glycerol solution have higher stability and EE across a diversity of temperatures and pHs, and a lower MIC of rifamycin S against S. aureus. MDPI 2022-11-28 /pmc/articles/PMC9785864/ /pubmed/36559121 http://dx.doi.org/10.3390/pharmaceutics14122626 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Marchianò, Verdiana Matos, Maria Marcet, Ismael Cabal, Maria Paz Gutiérrez, Gemma Blanco-López, Maria Carmen Stability of Non-Ionic Surfactant Vesicles Loaded with Rifamycin S |
title | Stability of Non-Ionic Surfactant Vesicles Loaded with Rifamycin S |
title_full | Stability of Non-Ionic Surfactant Vesicles Loaded with Rifamycin S |
title_fullStr | Stability of Non-Ionic Surfactant Vesicles Loaded with Rifamycin S |
title_full_unstemmed | Stability of Non-Ionic Surfactant Vesicles Loaded with Rifamycin S |
title_short | Stability of Non-Ionic Surfactant Vesicles Loaded with Rifamycin S |
title_sort | stability of non-ionic surfactant vesicles loaded with rifamycin s |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9785864/ https://www.ncbi.nlm.nih.gov/pubmed/36559121 http://dx.doi.org/10.3390/pharmaceutics14122626 |
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