Cargando…

Heparin Inhibits SARS-CoV-2 Replication in Human Nasal Epithelial Cells

SARS-CoV-2 is the causative agent of the COVID-19 pandemic. Vaccination, supported by social and public health measures, has proven efficacious for reducing disease severity and virus spread. However, the emergence of highly transmissible viral variants that escape prior immunity highlights the need...

Descripción completa

Detalles Bibliográficos
Autores principales: Lee, Leo Yi Yang, Suryadinata, Randy, McCafferty, Conor, Ignjatovic, Vera, Purcell, Damian F. J., Robinson, Phil, Morton, Craig J., Parker, Michael W., Anderson, Gary P., Monagle, Paul, Subbarao, Kanta, Neil, Jessica A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9785945/
https://www.ncbi.nlm.nih.gov/pubmed/36560624
http://dx.doi.org/10.3390/v14122620
_version_ 1784858172437037056
author Lee, Leo Yi Yang
Suryadinata, Randy
McCafferty, Conor
Ignjatovic, Vera
Purcell, Damian F. J.
Robinson, Phil
Morton, Craig J.
Parker, Michael W.
Anderson, Gary P.
Monagle, Paul
Subbarao, Kanta
Neil, Jessica A.
author_facet Lee, Leo Yi Yang
Suryadinata, Randy
McCafferty, Conor
Ignjatovic, Vera
Purcell, Damian F. J.
Robinson, Phil
Morton, Craig J.
Parker, Michael W.
Anderson, Gary P.
Monagle, Paul
Subbarao, Kanta
Neil, Jessica A.
author_sort Lee, Leo Yi Yang
collection PubMed
description SARS-CoV-2 is the causative agent of the COVID-19 pandemic. Vaccination, supported by social and public health measures, has proven efficacious for reducing disease severity and virus spread. However, the emergence of highly transmissible viral variants that escape prior immunity highlights the need for additional mitigation approaches. Heparin binds the SARS-CoV-2 spike protein and can inhibit virus entry and replication in susceptible human cell lines and bronchial epithelial cells. Primary infection predominantly occurs via the nasal epithelium, but the nasal cell biology of SARS-CoV-2 is not well studied. We hypothesized that prophylactic intranasal administration of heparin may provide strain-agnostic protection for household contacts or those in high-risk settings against SARS-CoV-2 infection. Therefore, we investigated the ability of heparin to inhibit SARS-CoV-2 infection and replication in differentiated human nasal epithelial cells and showed that prolonged exposure to heparin inhibits virus infection. Furthermore, we establish a method for PCR detection of SARS-CoV-2 viral genomes in heparin-treated samples that can be adapted for the detection of viruses in clinical studies.
format Online
Article
Text
id pubmed-9785945
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-97859452022-12-24 Heparin Inhibits SARS-CoV-2 Replication in Human Nasal Epithelial Cells Lee, Leo Yi Yang Suryadinata, Randy McCafferty, Conor Ignjatovic, Vera Purcell, Damian F. J. Robinson, Phil Morton, Craig J. Parker, Michael W. Anderson, Gary P. Monagle, Paul Subbarao, Kanta Neil, Jessica A. Viruses Article SARS-CoV-2 is the causative agent of the COVID-19 pandemic. Vaccination, supported by social and public health measures, has proven efficacious for reducing disease severity and virus spread. However, the emergence of highly transmissible viral variants that escape prior immunity highlights the need for additional mitigation approaches. Heparin binds the SARS-CoV-2 spike protein and can inhibit virus entry and replication in susceptible human cell lines and bronchial epithelial cells. Primary infection predominantly occurs via the nasal epithelium, but the nasal cell biology of SARS-CoV-2 is not well studied. We hypothesized that prophylactic intranasal administration of heparin may provide strain-agnostic protection for household contacts or those in high-risk settings against SARS-CoV-2 infection. Therefore, we investigated the ability of heparin to inhibit SARS-CoV-2 infection and replication in differentiated human nasal epithelial cells and showed that prolonged exposure to heparin inhibits virus infection. Furthermore, we establish a method for PCR detection of SARS-CoV-2 viral genomes in heparin-treated samples that can be adapted for the detection of viruses in clinical studies. MDPI 2022-11-24 /pmc/articles/PMC9785945/ /pubmed/36560624 http://dx.doi.org/10.3390/v14122620 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Leo Yi Yang
Suryadinata, Randy
McCafferty, Conor
Ignjatovic, Vera
Purcell, Damian F. J.
Robinson, Phil
Morton, Craig J.
Parker, Michael W.
Anderson, Gary P.
Monagle, Paul
Subbarao, Kanta
Neil, Jessica A.
Heparin Inhibits SARS-CoV-2 Replication in Human Nasal Epithelial Cells
title Heparin Inhibits SARS-CoV-2 Replication in Human Nasal Epithelial Cells
title_full Heparin Inhibits SARS-CoV-2 Replication in Human Nasal Epithelial Cells
title_fullStr Heparin Inhibits SARS-CoV-2 Replication in Human Nasal Epithelial Cells
title_full_unstemmed Heparin Inhibits SARS-CoV-2 Replication in Human Nasal Epithelial Cells
title_short Heparin Inhibits SARS-CoV-2 Replication in Human Nasal Epithelial Cells
title_sort heparin inhibits sars-cov-2 replication in human nasal epithelial cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9785945/
https://www.ncbi.nlm.nih.gov/pubmed/36560624
http://dx.doi.org/10.3390/v14122620
work_keys_str_mv AT leeleoyiyang heparininhibitssarscov2replicationinhumannasalepithelialcells
AT suryadinatarandy heparininhibitssarscov2replicationinhumannasalepithelialcells
AT mccaffertyconor heparininhibitssarscov2replicationinhumannasalepithelialcells
AT ignjatovicvera heparininhibitssarscov2replicationinhumannasalepithelialcells
AT purcelldamianfj heparininhibitssarscov2replicationinhumannasalepithelialcells
AT robinsonphil heparininhibitssarscov2replicationinhumannasalepithelialcells
AT mortoncraigj heparininhibitssarscov2replicationinhumannasalepithelialcells
AT parkermichaelw heparininhibitssarscov2replicationinhumannasalepithelialcells
AT andersongaryp heparininhibitssarscov2replicationinhumannasalepithelialcells
AT monaglepaul heparininhibitssarscov2replicationinhumannasalepithelialcells
AT subbaraokanta heparininhibitssarscov2replicationinhumannasalepithelialcells
AT neiljessicaa heparininhibitssarscov2replicationinhumannasalepithelialcells