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Highly Sensitive and Selective Graphene Nanoribbon Based Enzymatic Glucose Screen-Printed Electrochemical Sensor

A simple, sensitive, cost effective, and reliable enzymatic glucose biosensor was developed and tested. Nitrogen-doped heat-treated graphene oxide nanoribbons (N-htGONR) were used for modification of commercially available screen-printed carbon electrodes (SPCEs), together with MnO(2) and glucose ox...

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Autores principales: Gričar, Ema, Radić, Josip, Genorio, Boštjan, Kolar, Mitja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9786066/
https://www.ncbi.nlm.nih.gov/pubmed/36559958
http://dx.doi.org/10.3390/s22249590
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author Gričar, Ema
Radić, Josip
Genorio, Boštjan
Kolar, Mitja
author_facet Gričar, Ema
Radić, Josip
Genorio, Boštjan
Kolar, Mitja
author_sort Gričar, Ema
collection PubMed
description A simple, sensitive, cost effective, and reliable enzymatic glucose biosensor was developed and tested. Nitrogen-doped heat-treated graphene oxide nanoribbons (N-htGONR) were used for modification of commercially available screen-printed carbon electrodes (SPCEs), together with MnO(2) and glucose oxidase. The resulting sensors were optimized and used to detect glucose in a wide linear range (0.05–5.0 mM) by a simple amperometric method, where the limit of detection was determined to be 0.008 mM. (lifetime), and reproducibility studies were also carried out and yielded favorable results. The sensor was then tested against potential interfering species present in food and beverage samples before its application to real matrix. Spiked beer samples were analyzed (with glucose recovery between 93.5 and 103.5%) to demonstrate the suitability of the developed sensor towards real food and beverage sample applications.
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spelling pubmed-97860662022-12-24 Highly Sensitive and Selective Graphene Nanoribbon Based Enzymatic Glucose Screen-Printed Electrochemical Sensor Gričar, Ema Radić, Josip Genorio, Boštjan Kolar, Mitja Sensors (Basel) Article A simple, sensitive, cost effective, and reliable enzymatic glucose biosensor was developed and tested. Nitrogen-doped heat-treated graphene oxide nanoribbons (N-htGONR) were used for modification of commercially available screen-printed carbon electrodes (SPCEs), together with MnO(2) and glucose oxidase. The resulting sensors were optimized and used to detect glucose in a wide linear range (0.05–5.0 mM) by a simple amperometric method, where the limit of detection was determined to be 0.008 mM. (lifetime), and reproducibility studies were also carried out and yielded favorable results. The sensor was then tested against potential interfering species present in food and beverage samples before its application to real matrix. Spiked beer samples were analyzed (with glucose recovery between 93.5 and 103.5%) to demonstrate the suitability of the developed sensor towards real food and beverage sample applications. MDPI 2022-12-07 /pmc/articles/PMC9786066/ /pubmed/36559958 http://dx.doi.org/10.3390/s22249590 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gričar, Ema
Radić, Josip
Genorio, Boštjan
Kolar, Mitja
Highly Sensitive and Selective Graphene Nanoribbon Based Enzymatic Glucose Screen-Printed Electrochemical Sensor
title Highly Sensitive and Selective Graphene Nanoribbon Based Enzymatic Glucose Screen-Printed Electrochemical Sensor
title_full Highly Sensitive and Selective Graphene Nanoribbon Based Enzymatic Glucose Screen-Printed Electrochemical Sensor
title_fullStr Highly Sensitive and Selective Graphene Nanoribbon Based Enzymatic Glucose Screen-Printed Electrochemical Sensor
title_full_unstemmed Highly Sensitive and Selective Graphene Nanoribbon Based Enzymatic Glucose Screen-Printed Electrochemical Sensor
title_short Highly Sensitive and Selective Graphene Nanoribbon Based Enzymatic Glucose Screen-Printed Electrochemical Sensor
title_sort highly sensitive and selective graphene nanoribbon based enzymatic glucose screen-printed electrochemical sensor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9786066/
https://www.ncbi.nlm.nih.gov/pubmed/36559958
http://dx.doi.org/10.3390/s22249590
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