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Highly Sensitive and Selective Graphene Nanoribbon Based Enzymatic Glucose Screen-Printed Electrochemical Sensor
A simple, sensitive, cost effective, and reliable enzymatic glucose biosensor was developed and tested. Nitrogen-doped heat-treated graphene oxide nanoribbons (N-htGONR) were used for modification of commercially available screen-printed carbon electrodes (SPCEs), together with MnO(2) and glucose ox...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9786066/ https://www.ncbi.nlm.nih.gov/pubmed/36559958 http://dx.doi.org/10.3390/s22249590 |
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author | Gričar, Ema Radić, Josip Genorio, Boštjan Kolar, Mitja |
author_facet | Gričar, Ema Radić, Josip Genorio, Boštjan Kolar, Mitja |
author_sort | Gričar, Ema |
collection | PubMed |
description | A simple, sensitive, cost effective, and reliable enzymatic glucose biosensor was developed and tested. Nitrogen-doped heat-treated graphene oxide nanoribbons (N-htGONR) were used for modification of commercially available screen-printed carbon electrodes (SPCEs), together with MnO(2) and glucose oxidase. The resulting sensors were optimized and used to detect glucose in a wide linear range (0.05–5.0 mM) by a simple amperometric method, where the limit of detection was determined to be 0.008 mM. (lifetime), and reproducibility studies were also carried out and yielded favorable results. The sensor was then tested against potential interfering species present in food and beverage samples before its application to real matrix. Spiked beer samples were analyzed (with glucose recovery between 93.5 and 103.5%) to demonstrate the suitability of the developed sensor towards real food and beverage sample applications. |
format | Online Article Text |
id | pubmed-9786066 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97860662022-12-24 Highly Sensitive and Selective Graphene Nanoribbon Based Enzymatic Glucose Screen-Printed Electrochemical Sensor Gričar, Ema Radić, Josip Genorio, Boštjan Kolar, Mitja Sensors (Basel) Article A simple, sensitive, cost effective, and reliable enzymatic glucose biosensor was developed and tested. Nitrogen-doped heat-treated graphene oxide nanoribbons (N-htGONR) were used for modification of commercially available screen-printed carbon electrodes (SPCEs), together with MnO(2) and glucose oxidase. The resulting sensors were optimized and used to detect glucose in a wide linear range (0.05–5.0 mM) by a simple amperometric method, where the limit of detection was determined to be 0.008 mM. (lifetime), and reproducibility studies were also carried out and yielded favorable results. The sensor was then tested against potential interfering species present in food and beverage samples before its application to real matrix. Spiked beer samples were analyzed (with glucose recovery between 93.5 and 103.5%) to demonstrate the suitability of the developed sensor towards real food and beverage sample applications. MDPI 2022-12-07 /pmc/articles/PMC9786066/ /pubmed/36559958 http://dx.doi.org/10.3390/s22249590 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gričar, Ema Radić, Josip Genorio, Boštjan Kolar, Mitja Highly Sensitive and Selective Graphene Nanoribbon Based Enzymatic Glucose Screen-Printed Electrochemical Sensor |
title | Highly Sensitive and Selective Graphene Nanoribbon Based Enzymatic Glucose Screen-Printed Electrochemical Sensor |
title_full | Highly Sensitive and Selective Graphene Nanoribbon Based Enzymatic Glucose Screen-Printed Electrochemical Sensor |
title_fullStr | Highly Sensitive and Selective Graphene Nanoribbon Based Enzymatic Glucose Screen-Printed Electrochemical Sensor |
title_full_unstemmed | Highly Sensitive and Selective Graphene Nanoribbon Based Enzymatic Glucose Screen-Printed Electrochemical Sensor |
title_short | Highly Sensitive and Selective Graphene Nanoribbon Based Enzymatic Glucose Screen-Printed Electrochemical Sensor |
title_sort | highly sensitive and selective graphene nanoribbon based enzymatic glucose screen-printed electrochemical sensor |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9786066/ https://www.ncbi.nlm.nih.gov/pubmed/36559958 http://dx.doi.org/10.3390/s22249590 |
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