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Versatile Confocal Raman Imaging Microscope Built from Off-the-Shelf Opto-Mechanical Components
Confocal Raman microscopic (CRM) imaging has evolved to become a key tool for spatially resolved, compositional analysis and imaging, down to the μm-scale, and nowadays one may choose between numerous commercial instruments. That notwithstanding, situations may arise which exclude the use of a comme...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9786121/ https://www.ncbi.nlm.nih.gov/pubmed/36560382 http://dx.doi.org/10.3390/s222410013 |
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author | Diaz Barrero, Deseada Zeller, Genrich Schlösser, Magnus Bornschein, Beate Telle, Helmut H. |
author_facet | Diaz Barrero, Deseada Zeller, Genrich Schlösser, Magnus Bornschein, Beate Telle, Helmut H. |
author_sort | Diaz Barrero, Deseada |
collection | PubMed |
description | Confocal Raman microscopic (CRM) imaging has evolved to become a key tool for spatially resolved, compositional analysis and imaging, down to the μm-scale, and nowadays one may choose between numerous commercial instruments. That notwithstanding, situations may arise which exclude the use of a commercial instrument, e.g., if the analysis involves toxic or radioactive samples/environments; one may not wish to render an expensive instrument unusable for other uses, due to contamination. Therefore, custom-designed CRM instrumentation—being adaptable to hazardous conditions and providing operational flexibility—may be beneficial. Here, we describe a CRM setup, which is constructed nearly in its entirety from off-the-shelf optomechanical and optical components. The original aim was to develop a CRM suitable for the investigation of samples exposed to tritium. For increased flexibility, the CRM system incorporates optical fiber coupling to both the Raman excitation laser and the spectrometer. Lateral raster scans and axial profiling of samples are facilitated by the use of a motorized xyz-translation assembly. Besides the description of the construction and alignment of the CRM system, we also provide (i) the experimental evaluation of system performance (such as, e.g., spatial resolution) and (ii) examples of Raman raster maps and axial profiles of selected thin-film samples (such as, e.g., graphene sheets). |
format | Online Article Text |
id | pubmed-9786121 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97861212022-12-24 Versatile Confocal Raman Imaging Microscope Built from Off-the-Shelf Opto-Mechanical Components Diaz Barrero, Deseada Zeller, Genrich Schlösser, Magnus Bornschein, Beate Telle, Helmut H. Sensors (Basel) Article Confocal Raman microscopic (CRM) imaging has evolved to become a key tool for spatially resolved, compositional analysis and imaging, down to the μm-scale, and nowadays one may choose between numerous commercial instruments. That notwithstanding, situations may arise which exclude the use of a commercial instrument, e.g., if the analysis involves toxic or radioactive samples/environments; one may not wish to render an expensive instrument unusable for other uses, due to contamination. Therefore, custom-designed CRM instrumentation—being adaptable to hazardous conditions and providing operational flexibility—may be beneficial. Here, we describe a CRM setup, which is constructed nearly in its entirety from off-the-shelf optomechanical and optical components. The original aim was to develop a CRM suitable for the investigation of samples exposed to tritium. For increased flexibility, the CRM system incorporates optical fiber coupling to both the Raman excitation laser and the spectrometer. Lateral raster scans and axial profiling of samples are facilitated by the use of a motorized xyz-translation assembly. Besides the description of the construction and alignment of the CRM system, we also provide (i) the experimental evaluation of system performance (such as, e.g., spatial resolution) and (ii) examples of Raman raster maps and axial profiles of selected thin-film samples (such as, e.g., graphene sheets). MDPI 2022-12-19 /pmc/articles/PMC9786121/ /pubmed/36560382 http://dx.doi.org/10.3390/s222410013 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Diaz Barrero, Deseada Zeller, Genrich Schlösser, Magnus Bornschein, Beate Telle, Helmut H. Versatile Confocal Raman Imaging Microscope Built from Off-the-Shelf Opto-Mechanical Components |
title | Versatile Confocal Raman Imaging Microscope Built from Off-the-Shelf Opto-Mechanical Components |
title_full | Versatile Confocal Raman Imaging Microscope Built from Off-the-Shelf Opto-Mechanical Components |
title_fullStr | Versatile Confocal Raman Imaging Microscope Built from Off-the-Shelf Opto-Mechanical Components |
title_full_unstemmed | Versatile Confocal Raman Imaging Microscope Built from Off-the-Shelf Opto-Mechanical Components |
title_short | Versatile Confocal Raman Imaging Microscope Built from Off-the-Shelf Opto-Mechanical Components |
title_sort | versatile confocal raman imaging microscope built from off-the-shelf opto-mechanical components |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9786121/ https://www.ncbi.nlm.nih.gov/pubmed/36560382 http://dx.doi.org/10.3390/s222410013 |
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