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Determining the expression levels of CSF‐1 and OCT4 , CREM‐1 , and protamine in testicular biopsies of adult Klinefelter patients: Their possible correlation with spermatogenesis

Klinefelter syndrome (KS) is the most prevalent genetic disorder of infertile males. This study aimed to determine in Klinefelter patients (KS) the expression levels of spermatogenic markers and testicular growth factors that might predict spermatogenesis based on conventional testicular sperm extra...

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Autores principales: Abofoul‐Azab, Maram, Lunenfeld, Eitan, Kleiman, Sandra, Barda, Shimi, Hauser, Ron, Huleihel, Mahmoud
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9786270/
https://www.ncbi.nlm.nih.gov/pubmed/36177809
http://dx.doi.org/10.1111/and.14558
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author Abofoul‐Azab, Maram
Lunenfeld, Eitan
Kleiman, Sandra
Barda, Shimi
Hauser, Ron
Huleihel, Mahmoud
author_facet Abofoul‐Azab, Maram
Lunenfeld, Eitan
Kleiman, Sandra
Barda, Shimi
Hauser, Ron
Huleihel, Mahmoud
author_sort Abofoul‐Azab, Maram
collection PubMed
description Klinefelter syndrome (KS) is the most prevalent genetic disorder of infertile males. This study aimed to determine in Klinefelter patients (KS) the expression levels of spermatogenic markers and testicular growth factors that might predict spermatogenesis based on conventional testicular sperm extraction (TESE). The expression levels of the pre‐meiotic (OCT4, CD9, GFR‐α1, α‐6‐INTEGRIN, SALL4, C‐KIT), meiotic (CREM‐1), and post‐meiotic (protamine) markers, as well as the colony stimulating factor‐1 (CSF‐1) were examined in testicular biopsies with and without mature sperm of KS and normal karyotype of azoospermic patients (AZO) with complete spermatogenesis. In the biopsies of AZO, the expression levels (fold of expression compared to the PPI of the same sample) of OCT4 were 9.68± 7.93, CREM 42.78± 28.22, CSF‐1 3.07 ± 3.19, and protamine 78498.12 ± 73214.40. Biopsies from KS included 7 with sperm and 17 without sperm. Among the biopsies with sperm, the expression levels of OCT4 were 7.27± 9.29, CREM 3.13± 7.89, CSF‐1 35.5 ± 48.01, and protamine 902.97 ± 2365.92. In 14 biopsies without sperm, we found low expression levels of OCT4, CREM and CSF‐1, and no expression of protamine. However, in three of the biopsies without sperm that highly expressed OCT4 and CSF‐1, the expression levels of CREM‐1 and protamine were high. These results may be used for further consulting with patients considering repeating conventional TESE or micro TESE and cryopreservation for possible future in‐vitro spermatogenesis.
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spelling pubmed-97862702022-12-27 Determining the expression levels of CSF‐1 and OCT4 , CREM‐1 , and protamine in testicular biopsies of adult Klinefelter patients: Their possible correlation with spermatogenesis Abofoul‐Azab, Maram Lunenfeld, Eitan Kleiman, Sandra Barda, Shimi Hauser, Ron Huleihel, Mahmoud Andrologia Original Articles Klinefelter syndrome (KS) is the most prevalent genetic disorder of infertile males. This study aimed to determine in Klinefelter patients (KS) the expression levels of spermatogenic markers and testicular growth factors that might predict spermatogenesis based on conventional testicular sperm extraction (TESE). The expression levels of the pre‐meiotic (OCT4, CD9, GFR‐α1, α‐6‐INTEGRIN, SALL4, C‐KIT), meiotic (CREM‐1), and post‐meiotic (protamine) markers, as well as the colony stimulating factor‐1 (CSF‐1) were examined in testicular biopsies with and without mature sperm of KS and normal karyotype of azoospermic patients (AZO) with complete spermatogenesis. In the biopsies of AZO, the expression levels (fold of expression compared to the PPI of the same sample) of OCT4 were 9.68± 7.93, CREM 42.78± 28.22, CSF‐1 3.07 ± 3.19, and protamine 78498.12 ± 73214.40. Biopsies from KS included 7 with sperm and 17 without sperm. Among the biopsies with sperm, the expression levels of OCT4 were 7.27± 9.29, CREM 3.13± 7.89, CSF‐1 35.5 ± 48.01, and protamine 902.97 ± 2365.92. In 14 biopsies without sperm, we found low expression levels of OCT4, CREM and CSF‐1, and no expression of protamine. However, in three of the biopsies without sperm that highly expressed OCT4 and CSF‐1, the expression levels of CREM‐1 and protamine were high. These results may be used for further consulting with patients considering repeating conventional TESE or micro TESE and cryopreservation for possible future in‐vitro spermatogenesis. John Wiley and Sons Inc. 2022-08-19 2022-11 /pmc/articles/PMC9786270/ /pubmed/36177809 http://dx.doi.org/10.1111/and.14558 Text en © 2022 The Authors. Andrologia published by Wiley‐VCH GmbH. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Abofoul‐Azab, Maram
Lunenfeld, Eitan
Kleiman, Sandra
Barda, Shimi
Hauser, Ron
Huleihel, Mahmoud
Determining the expression levels of CSF‐1 and OCT4 , CREM‐1 , and protamine in testicular biopsies of adult Klinefelter patients: Their possible correlation with spermatogenesis
title Determining the expression levels of CSF‐1 and OCT4 , CREM‐1 , and protamine in testicular biopsies of adult Klinefelter patients: Their possible correlation with spermatogenesis
title_full Determining the expression levels of CSF‐1 and OCT4 , CREM‐1 , and protamine in testicular biopsies of adult Klinefelter patients: Their possible correlation with spermatogenesis
title_fullStr Determining the expression levels of CSF‐1 and OCT4 , CREM‐1 , and protamine in testicular biopsies of adult Klinefelter patients: Their possible correlation with spermatogenesis
title_full_unstemmed Determining the expression levels of CSF‐1 and OCT4 , CREM‐1 , and protamine in testicular biopsies of adult Klinefelter patients: Their possible correlation with spermatogenesis
title_short Determining the expression levels of CSF‐1 and OCT4 , CREM‐1 , and protamine in testicular biopsies of adult Klinefelter patients: Their possible correlation with spermatogenesis
title_sort determining the expression levels of csf‐1 and oct4 , crem‐1 , and protamine in testicular biopsies of adult klinefelter patients: their possible correlation with spermatogenesis
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9786270/
https://www.ncbi.nlm.nih.gov/pubmed/36177809
http://dx.doi.org/10.1111/and.14558
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