Multiomic Mass Spectrometry Imaging to Advance Future Pathological Understanding of Ocular Disease

Determining the locations of proteins within the eye thought to be involved in ocular pathogenesis is important to determine how best to target them for therapeutic benefits. However, immunohistochemistry is limited by the availability and specificity of antibodies. Additionally, the perceived role...

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Autores principales: Millar, Joshua, Ozaki, Ema, Campbell, Susan, Duckett, Catherine, Doyle, Sarah, Cole, Laura M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9786289/
https://www.ncbi.nlm.nih.gov/pubmed/36557277
http://dx.doi.org/10.3390/metabo12121239
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author Millar, Joshua
Ozaki, Ema
Campbell, Susan
Duckett, Catherine
Doyle, Sarah
Cole, Laura M.
author_facet Millar, Joshua
Ozaki, Ema
Campbell, Susan
Duckett, Catherine
Doyle, Sarah
Cole, Laura M.
author_sort Millar, Joshua
collection PubMed
description Determining the locations of proteins within the eye thought to be involved in ocular pathogenesis is important to determine how best to target them for therapeutic benefits. However, immunohistochemistry is limited by the availability and specificity of antibodies. Additionally, the perceived role of both essential and non-essential metals within ocular tissue has been at the forefront of age-related macular degeneration (AMD) pathology for decades, yet even key metals such as copper and zinc have yet to have their roles deconvoluted. Here, mass spectrometry imaging (MSI) is employed to identify and spatially characterize both proteomic and metallomic species within ocular tissue to advance the application of a multiomic imaging methodology for the investigation of ocular diseases.
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spelling pubmed-97862892022-12-24 Multiomic Mass Spectrometry Imaging to Advance Future Pathological Understanding of Ocular Disease Millar, Joshua Ozaki, Ema Campbell, Susan Duckett, Catherine Doyle, Sarah Cole, Laura M. Metabolites Communication Determining the locations of proteins within the eye thought to be involved in ocular pathogenesis is important to determine how best to target them for therapeutic benefits. However, immunohistochemistry is limited by the availability and specificity of antibodies. Additionally, the perceived role of both essential and non-essential metals within ocular tissue has been at the forefront of age-related macular degeneration (AMD) pathology for decades, yet even key metals such as copper and zinc have yet to have their roles deconvoluted. Here, mass spectrometry imaging (MSI) is employed to identify and spatially characterize both proteomic and metallomic species within ocular tissue to advance the application of a multiomic imaging methodology for the investigation of ocular diseases. MDPI 2022-12-09 /pmc/articles/PMC9786289/ /pubmed/36557277 http://dx.doi.org/10.3390/metabo12121239 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Millar, Joshua
Ozaki, Ema
Campbell, Susan
Duckett, Catherine
Doyle, Sarah
Cole, Laura M.
Multiomic Mass Spectrometry Imaging to Advance Future Pathological Understanding of Ocular Disease
title Multiomic Mass Spectrometry Imaging to Advance Future Pathological Understanding of Ocular Disease
title_full Multiomic Mass Spectrometry Imaging to Advance Future Pathological Understanding of Ocular Disease
title_fullStr Multiomic Mass Spectrometry Imaging to Advance Future Pathological Understanding of Ocular Disease
title_full_unstemmed Multiomic Mass Spectrometry Imaging to Advance Future Pathological Understanding of Ocular Disease
title_short Multiomic Mass Spectrometry Imaging to Advance Future Pathological Understanding of Ocular Disease
title_sort multiomic mass spectrometry imaging to advance future pathological understanding of ocular disease
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9786289/
https://www.ncbi.nlm.nih.gov/pubmed/36557277
http://dx.doi.org/10.3390/metabo12121239
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