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A Simple Preparation Method of Gelatin Hydrogels Incorporating Cisplatin for Sustained Release
The objective of this study was to develop a new preparation method for cisplatin (CDDP)-incorporated gelatin hydrogels without using chemical crosslinking nor a vacuum heating instrument for dehydrothermal crosslinking. By simply mixing CDDP and gelatin, CDDP-crosslinked gelatin hydrogels (CCGH) we...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9786307/ https://www.ncbi.nlm.nih.gov/pubmed/36559095 http://dx.doi.org/10.3390/pharmaceutics14122601 |
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author | Suzuki, Takahisa Tsunoda, Shigeru Yamashita, Kota Kuwahara, Toshie Ando, Mitsuru Tabata, Yasuhiko Obama, Kazutaka |
author_facet | Suzuki, Takahisa Tsunoda, Shigeru Yamashita, Kota Kuwahara, Toshie Ando, Mitsuru Tabata, Yasuhiko Obama, Kazutaka |
author_sort | Suzuki, Takahisa |
collection | PubMed |
description | The objective of this study was to develop a new preparation method for cisplatin (CDDP)-incorporated gelatin hydrogels without using chemical crosslinking nor a vacuum heating instrument for dehydrothermal crosslinking. By simply mixing CDDP and gelatin, CDDP-crosslinked gelatin hydrogels (CCGH) were prepared. CDDP functions as a crosslinking agent of gelatin to form the gelatin hydrogel. Simultaneously, CDDP is incorporated into the gelatin hydrogel as a controlled release carrier. CDDP’s in vitro and in vivo anticancer efficacy after incorporation into CCGH was evaluated. In the in vitro system, the CDDP was released gradually due to CCGH degradation with an initial burst release of approximately 16%. CDDP metal-coordinated with the degraded fragment of gelatin was released from CCGH with maintaining the anticancer activity. After intraperitoneal administration of CCGH, CDDP was detected in the blood circulation while its toxicity was low. Following intraperitoneal administration of CCGH in a murine peritoneal dissemination model of human gastric cancer MKN45-Luc cell line, the survival time was significantly prolonged compared with free CDDP solution. It is concluded that CCGH prepared by the CDDP-based crosslinking of gelatin is an excellent sustained release system of CDDP to achieve superior anticancer effects with minimal side effects compared with free CDDP solution. |
format | Online Article Text |
id | pubmed-9786307 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97863072022-12-24 A Simple Preparation Method of Gelatin Hydrogels Incorporating Cisplatin for Sustained Release Suzuki, Takahisa Tsunoda, Shigeru Yamashita, Kota Kuwahara, Toshie Ando, Mitsuru Tabata, Yasuhiko Obama, Kazutaka Pharmaceutics Article The objective of this study was to develop a new preparation method for cisplatin (CDDP)-incorporated gelatin hydrogels without using chemical crosslinking nor a vacuum heating instrument for dehydrothermal crosslinking. By simply mixing CDDP and gelatin, CDDP-crosslinked gelatin hydrogels (CCGH) were prepared. CDDP functions as a crosslinking agent of gelatin to form the gelatin hydrogel. Simultaneously, CDDP is incorporated into the gelatin hydrogel as a controlled release carrier. CDDP’s in vitro and in vivo anticancer efficacy after incorporation into CCGH was evaluated. In the in vitro system, the CDDP was released gradually due to CCGH degradation with an initial burst release of approximately 16%. CDDP metal-coordinated with the degraded fragment of gelatin was released from CCGH with maintaining the anticancer activity. After intraperitoneal administration of CCGH, CDDP was detected in the blood circulation while its toxicity was low. Following intraperitoneal administration of CCGH in a murine peritoneal dissemination model of human gastric cancer MKN45-Luc cell line, the survival time was significantly prolonged compared with free CDDP solution. It is concluded that CCGH prepared by the CDDP-based crosslinking of gelatin is an excellent sustained release system of CDDP to achieve superior anticancer effects with minimal side effects compared with free CDDP solution. MDPI 2022-11-25 /pmc/articles/PMC9786307/ /pubmed/36559095 http://dx.doi.org/10.3390/pharmaceutics14122601 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Suzuki, Takahisa Tsunoda, Shigeru Yamashita, Kota Kuwahara, Toshie Ando, Mitsuru Tabata, Yasuhiko Obama, Kazutaka A Simple Preparation Method of Gelatin Hydrogels Incorporating Cisplatin for Sustained Release |
title | A Simple Preparation Method of Gelatin Hydrogels Incorporating Cisplatin for Sustained Release |
title_full | A Simple Preparation Method of Gelatin Hydrogels Incorporating Cisplatin for Sustained Release |
title_fullStr | A Simple Preparation Method of Gelatin Hydrogels Incorporating Cisplatin for Sustained Release |
title_full_unstemmed | A Simple Preparation Method of Gelatin Hydrogels Incorporating Cisplatin for Sustained Release |
title_short | A Simple Preparation Method of Gelatin Hydrogels Incorporating Cisplatin for Sustained Release |
title_sort | simple preparation method of gelatin hydrogels incorporating cisplatin for sustained release |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9786307/ https://www.ncbi.nlm.nih.gov/pubmed/36559095 http://dx.doi.org/10.3390/pharmaceutics14122601 |
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