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New Wolbachia pipientis Genotype Increasing Heat Stress Resistance of Drosophila melanogaster Host Is Characterized by a Large Chromosomal Inversion

The maternally transmitted endocellular bacteria Wolbachia is a well-known symbiont of insects, demonstrating both negative and positive effects on host fitness. The previously found Wolbachia strain wMelPlus is characterized by a positive effect on the stress-resistance of its host Drosophila melan...

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Autores principales: Korenskaia, Aleksandra E., Shishkina, Olga D., Klimenko, Alexandra I., Andreenkova, Olga V., Bobrovskikh, Margarita A., Shatskaya, Natalja V., Vasiliev, Gennady V., Gruntenko, Nataly E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9786649/
https://www.ncbi.nlm.nih.gov/pubmed/36555851
http://dx.doi.org/10.3390/ijms232416212
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author Korenskaia, Aleksandra E.
Shishkina, Olga D.
Klimenko, Alexandra I.
Andreenkova, Olga V.
Bobrovskikh, Margarita A.
Shatskaya, Natalja V.
Vasiliev, Gennady V.
Gruntenko, Nataly E.
author_facet Korenskaia, Aleksandra E.
Shishkina, Olga D.
Klimenko, Alexandra I.
Andreenkova, Olga V.
Bobrovskikh, Margarita A.
Shatskaya, Natalja V.
Vasiliev, Gennady V.
Gruntenko, Nataly E.
author_sort Korenskaia, Aleksandra E.
collection PubMed
description The maternally transmitted endocellular bacteria Wolbachia is a well-known symbiont of insects, demonstrating both negative and positive effects on host fitness. The previously found Wolbachia strain wMelPlus is characterized by a positive effect on the stress-resistance of its host Drosophila melanogaster, under heat stress conditions. This investigation is dedicated to studying the genomic underpinnings of such an effect. We sequenced two closely related Wolbachia strains, wMelPlus and wMelCS(112), assembled their complete genomes, and performed comparative genomic analysis engaging available Wolbachia genomes from the wMel and wMelCS groups. Despite the two strains under study sharing very close gene-composition, we discovered a large (>1/6 of total genome) chromosomal inversion in wMelPlus, spanning through the region that includes the area of the inversion earlier found in the wMel group of Wolbachia genotypes. A number of genes in unique inversion blocks of wMelPlus were identified that might be involved in the induction of a stress-resistant phenotype in the host. We hypothesize that such an inversion could rearrange established genetic regulatory-networks, causing the observed effects of such a complex fly phenotype as a modulation of heat stress resistance. Based on our findings, we propose that wMelPlus be distinguished as a separate genotype of the wMelCS group, named wMelCS3.
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spelling pubmed-97866492022-12-24 New Wolbachia pipientis Genotype Increasing Heat Stress Resistance of Drosophila melanogaster Host Is Characterized by a Large Chromosomal Inversion Korenskaia, Aleksandra E. Shishkina, Olga D. Klimenko, Alexandra I. Andreenkova, Olga V. Bobrovskikh, Margarita A. Shatskaya, Natalja V. Vasiliev, Gennady V. Gruntenko, Nataly E. Int J Mol Sci Article The maternally transmitted endocellular bacteria Wolbachia is a well-known symbiont of insects, demonstrating both negative and positive effects on host fitness. The previously found Wolbachia strain wMelPlus is characterized by a positive effect on the stress-resistance of its host Drosophila melanogaster, under heat stress conditions. This investigation is dedicated to studying the genomic underpinnings of such an effect. We sequenced two closely related Wolbachia strains, wMelPlus and wMelCS(112), assembled their complete genomes, and performed comparative genomic analysis engaging available Wolbachia genomes from the wMel and wMelCS groups. Despite the two strains under study sharing very close gene-composition, we discovered a large (>1/6 of total genome) chromosomal inversion in wMelPlus, spanning through the region that includes the area of the inversion earlier found in the wMel group of Wolbachia genotypes. A number of genes in unique inversion blocks of wMelPlus were identified that might be involved in the induction of a stress-resistant phenotype in the host. We hypothesize that such an inversion could rearrange established genetic regulatory-networks, causing the observed effects of such a complex fly phenotype as a modulation of heat stress resistance. Based on our findings, we propose that wMelPlus be distinguished as a separate genotype of the wMelCS group, named wMelCS3. MDPI 2022-12-19 /pmc/articles/PMC9786649/ /pubmed/36555851 http://dx.doi.org/10.3390/ijms232416212 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Korenskaia, Aleksandra E.
Shishkina, Olga D.
Klimenko, Alexandra I.
Andreenkova, Olga V.
Bobrovskikh, Margarita A.
Shatskaya, Natalja V.
Vasiliev, Gennady V.
Gruntenko, Nataly E.
New Wolbachia pipientis Genotype Increasing Heat Stress Resistance of Drosophila melanogaster Host Is Characterized by a Large Chromosomal Inversion
title New Wolbachia pipientis Genotype Increasing Heat Stress Resistance of Drosophila melanogaster Host Is Characterized by a Large Chromosomal Inversion
title_full New Wolbachia pipientis Genotype Increasing Heat Stress Resistance of Drosophila melanogaster Host Is Characterized by a Large Chromosomal Inversion
title_fullStr New Wolbachia pipientis Genotype Increasing Heat Stress Resistance of Drosophila melanogaster Host Is Characterized by a Large Chromosomal Inversion
title_full_unstemmed New Wolbachia pipientis Genotype Increasing Heat Stress Resistance of Drosophila melanogaster Host Is Characterized by a Large Chromosomal Inversion
title_short New Wolbachia pipientis Genotype Increasing Heat Stress Resistance of Drosophila melanogaster Host Is Characterized by a Large Chromosomal Inversion
title_sort new wolbachia pipientis genotype increasing heat stress resistance of drosophila melanogaster host is characterized by a large chromosomal inversion
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9786649/
https://www.ncbi.nlm.nih.gov/pubmed/36555851
http://dx.doi.org/10.3390/ijms232416212
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