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Characterization of Femtosecond Laser and Porcine Crystalline Lens Interactions by Optical Microscopy

The use of ultrafast laser pulses for eye anterior segment surgery has seen a tremendous growth of interest as the technique has revolutionized the field, from the treatment of myopia, hyperopia, and presbyopia in the cornea to laser-assisted cataract surgery of the crystalline lens. For the latter,...

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Autores principales: Ben Moussa, Olfa, Talbi, Abderazek, Poinard, Sylvain, Garcin, Thibaud, Gauthier, Anne-Sophie, Thuret, Gilles, Gain, Philippe, Maurer, Aurélien, Sedao, Xxx, Mauclair, Cyril
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9786683/
https://www.ncbi.nlm.nih.gov/pubmed/36557426
http://dx.doi.org/10.3390/mi13122128
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author Ben Moussa, Olfa
Talbi, Abderazek
Poinard, Sylvain
Garcin, Thibaud
Gauthier, Anne-Sophie
Thuret, Gilles
Gain, Philippe
Maurer, Aurélien
Sedao, Xxx
Mauclair, Cyril
author_facet Ben Moussa, Olfa
Talbi, Abderazek
Poinard, Sylvain
Garcin, Thibaud
Gauthier, Anne-Sophie
Thuret, Gilles
Gain, Philippe
Maurer, Aurélien
Sedao, Xxx
Mauclair, Cyril
author_sort Ben Moussa, Olfa
collection PubMed
description The use of ultrafast laser pulses for eye anterior segment surgery has seen a tremendous growth of interest as the technique has revolutionized the field, from the treatment of myopia, hyperopia, and presbyopia in the cornea to laser-assisted cataract surgery of the crystalline lens. For the latter, a comprehensive understanding of the laser–tissue interaction has yet to be achieved, mainly because of the challenge of observing the interaction zone in situ with sufficient spatial and temporal resolution in the complex and multi-layered tissue of the crystalline lens. We report here on the dedicated characterization results of the laser–tissue interaction zone in the ex vivo porcine lens using three different methods: in situ and real-time microscopy, wide-field optical imaging, and phase-contrast microscopy of the histological cross sections. These complementary approaches together revealed new physical and biological consequences of laser irradiation: a low-energy interaction regime (pulse energy below ~1 µJ) with very limited cavitation effects and a stronger photo-disruption regime (pulse energy above 1 µJ) with a long cavitation duration from seconds to minutes, resulting in elongated spots. These advances in the understanding of the ultrafast laser’s interactions with the lens are of the utmost importance for the preparation of the next-generation treatments that will be applied to the lens.
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spelling pubmed-97866832022-12-24 Characterization of Femtosecond Laser and Porcine Crystalline Lens Interactions by Optical Microscopy Ben Moussa, Olfa Talbi, Abderazek Poinard, Sylvain Garcin, Thibaud Gauthier, Anne-Sophie Thuret, Gilles Gain, Philippe Maurer, Aurélien Sedao, Xxx Mauclair, Cyril Micromachines (Basel) Article The use of ultrafast laser pulses for eye anterior segment surgery has seen a tremendous growth of interest as the technique has revolutionized the field, from the treatment of myopia, hyperopia, and presbyopia in the cornea to laser-assisted cataract surgery of the crystalline lens. For the latter, a comprehensive understanding of the laser–tissue interaction has yet to be achieved, mainly because of the challenge of observing the interaction zone in situ with sufficient spatial and temporal resolution in the complex and multi-layered tissue of the crystalline lens. We report here on the dedicated characterization results of the laser–tissue interaction zone in the ex vivo porcine lens using three different methods: in situ and real-time microscopy, wide-field optical imaging, and phase-contrast microscopy of the histological cross sections. These complementary approaches together revealed new physical and biological consequences of laser irradiation: a low-energy interaction regime (pulse energy below ~1 µJ) with very limited cavitation effects and a stronger photo-disruption regime (pulse energy above 1 µJ) with a long cavitation duration from seconds to minutes, resulting in elongated spots. These advances in the understanding of the ultrafast laser’s interactions with the lens are of the utmost importance for the preparation of the next-generation treatments that will be applied to the lens. MDPI 2022-12-01 /pmc/articles/PMC9786683/ /pubmed/36557426 http://dx.doi.org/10.3390/mi13122128 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ben Moussa, Olfa
Talbi, Abderazek
Poinard, Sylvain
Garcin, Thibaud
Gauthier, Anne-Sophie
Thuret, Gilles
Gain, Philippe
Maurer, Aurélien
Sedao, Xxx
Mauclair, Cyril
Characterization of Femtosecond Laser and Porcine Crystalline Lens Interactions by Optical Microscopy
title Characterization of Femtosecond Laser and Porcine Crystalline Lens Interactions by Optical Microscopy
title_full Characterization of Femtosecond Laser and Porcine Crystalline Lens Interactions by Optical Microscopy
title_fullStr Characterization of Femtosecond Laser and Porcine Crystalline Lens Interactions by Optical Microscopy
title_full_unstemmed Characterization of Femtosecond Laser and Porcine Crystalline Lens Interactions by Optical Microscopy
title_short Characterization of Femtosecond Laser and Porcine Crystalline Lens Interactions by Optical Microscopy
title_sort characterization of femtosecond laser and porcine crystalline lens interactions by optical microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9786683/
https://www.ncbi.nlm.nih.gov/pubmed/36557426
http://dx.doi.org/10.3390/mi13122128
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