Cargando…
Single Camera-Based Dual-Channel Near-Infrared Fluorescence Imaging system
In this study, we propose a single camera-based dual-channel near-infrared (NIR) fluorescence imaging system that produces color and dual-channel NIR fluorescence images in real time. To simultaneously acquire color and dual-channel NIR fluorescence images of two fluorescent agents, three cameras an...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9786791/ https://www.ncbi.nlm.nih.gov/pubmed/36560127 http://dx.doi.org/10.3390/s22249758 |
_version_ | 1784858371449421824 |
---|---|
author | Choi, Janghoon Shin, Jun-Geun Tak, Yoon-Oh Seo, Youngseok Eom, Jonghyun |
author_facet | Choi, Janghoon Shin, Jun-Geun Tak, Yoon-Oh Seo, Youngseok Eom, Jonghyun |
author_sort | Choi, Janghoon |
collection | PubMed |
description | In this study, we propose a single camera-based dual-channel near-infrared (NIR) fluorescence imaging system that produces color and dual-channel NIR fluorescence images in real time. To simultaneously acquire color and dual-channel NIR fluorescence images of two fluorescent agents, three cameras and additional optical parts are generally used. As a result, the volume of the image acquisition unit increases, interfering with movements during surgical procedures and increasing production costs. In the system herein proposed, instead of using three cameras, we set a single camera equipped with two image sensors that can simultaneously acquire color and single-channel NIR fluorescence images, thus reducing the volume of the image acquisition unit. The single-channel NIR fluorescence images were time-divided into two channels by synchronizing the camera and two excitation lasers, and the noise caused by the crosstalk effect between the two fluorescent agents was removed through image processing. To evaluate the performance of the system, experiments were conducted for the two fluorescent agents to measure the sensitivity, crosstalk effect, and signal-to-background ratio. The compactness of the resulting image acquisition unit alleviates the inconvenient movement obstruction of previous devices during clinical and animal surgery and reduces the complexity and costs of the manufacturing process, which may facilitate the dissemination of this type of system. |
format | Online Article Text |
id | pubmed-9786791 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97867912022-12-24 Single Camera-Based Dual-Channel Near-Infrared Fluorescence Imaging system Choi, Janghoon Shin, Jun-Geun Tak, Yoon-Oh Seo, Youngseok Eom, Jonghyun Sensors (Basel) Article In this study, we propose a single camera-based dual-channel near-infrared (NIR) fluorescence imaging system that produces color and dual-channel NIR fluorescence images in real time. To simultaneously acquire color and dual-channel NIR fluorescence images of two fluorescent agents, three cameras and additional optical parts are generally used. As a result, the volume of the image acquisition unit increases, interfering with movements during surgical procedures and increasing production costs. In the system herein proposed, instead of using three cameras, we set a single camera equipped with two image sensors that can simultaneously acquire color and single-channel NIR fluorescence images, thus reducing the volume of the image acquisition unit. The single-channel NIR fluorescence images were time-divided into two channels by synchronizing the camera and two excitation lasers, and the noise caused by the crosstalk effect between the two fluorescent agents was removed through image processing. To evaluate the performance of the system, experiments were conducted for the two fluorescent agents to measure the sensitivity, crosstalk effect, and signal-to-background ratio. The compactness of the resulting image acquisition unit alleviates the inconvenient movement obstruction of previous devices during clinical and animal surgery and reduces the complexity and costs of the manufacturing process, which may facilitate the dissemination of this type of system. MDPI 2022-12-13 /pmc/articles/PMC9786791/ /pubmed/36560127 http://dx.doi.org/10.3390/s22249758 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Choi, Janghoon Shin, Jun-Geun Tak, Yoon-Oh Seo, Youngseok Eom, Jonghyun Single Camera-Based Dual-Channel Near-Infrared Fluorescence Imaging system |
title | Single Camera-Based Dual-Channel Near-Infrared Fluorescence Imaging system |
title_full | Single Camera-Based Dual-Channel Near-Infrared Fluorescence Imaging system |
title_fullStr | Single Camera-Based Dual-Channel Near-Infrared Fluorescence Imaging system |
title_full_unstemmed | Single Camera-Based Dual-Channel Near-Infrared Fluorescence Imaging system |
title_short | Single Camera-Based Dual-Channel Near-Infrared Fluorescence Imaging system |
title_sort | single camera-based dual-channel near-infrared fluorescence imaging system |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9786791/ https://www.ncbi.nlm.nih.gov/pubmed/36560127 http://dx.doi.org/10.3390/s22249758 |
work_keys_str_mv | AT choijanghoon singlecamerabaseddualchannelnearinfraredfluorescenceimagingsystem AT shinjungeun singlecamerabaseddualchannelnearinfraredfluorescenceimagingsystem AT takyoonoh singlecamerabaseddualchannelnearinfraredfluorescenceimagingsystem AT seoyoungseok singlecamerabaseddualchannelnearinfraredfluorescenceimagingsystem AT eomjonghyun singlecamerabaseddualchannelnearinfraredfluorescenceimagingsystem |